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Physicochemical, antioxidant, antibacterial and antibiofilm activity of Carum copticum essential oil nanoemulsion on Escherichia coli O157:H7 and Listeria monocytogenes

Document Type : Original Article

Authors

Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Carum copticum essential oil (CEO) is used to prevent the growth of food-borne pathogens. The Carum copticum essential oil nanoemulsion (CEON) was prepared using low energy sonication at 0, 2.50, 5.00 and 10 min based on surfactant to-oil ratio (SOR=1). Chemical composition, antimicrobial and antibiofilm properties of CEON were examined. Our data showed that the average diameter of the droplets of CEON was between 46.89 and 120.90 nm. The MICs of CEON and CEO against E. coli O157:H7 and L. monocytogenes were tested. L. monocytogenes was more sensitive than E. coli O157:H7. The sonication time and the total viable bacteria (TVC) in the study were inversely related to each other. Furthermore, CEON at the 4.00 × MIC concentration and contact time of 20 min caused 77.14% and 67.03% reduction of E. coli O157:H7 and L. monocytogenes biofilms, respectively. The antibiofilm activity of CEO was significantly lower than CEON and caused a 62.60% and 43.86% reduction of E. coli O157: H7 and L. monocytogenes biofilms, respectively. The results showed that CEON produced by low energy sonication would have a higher antibacterial efficiency than non-encapsulted essential oil.

Keywords

References
  1. Fang Z, Warner RD, Johnson SK et al. Active and intelligent packaging in meat industry. Trends Food Sci Technol 2017; 61: 60-71.
  2. Shahbazi Y. Antilisterial effects of Ziziphora clinopodioides essential oil and nisin in milk. J Pure Appl Microbiol 2015; 9(3): 1993-2000.‏
  3. Esmaeli F, Tajik H, Mehdizadeh T, et al. Effect of combined application of Pimpinella affinis essential oil and extract in zein edible coating on vacuum packaged rainbow trout fillet quality. Vet Res Forum 2019; 10(2): 109-117.
  4. Hashemi M, Ehsani A, Hosseini Jazani N, et al. Chemical composition and in vitro antibacterial activity of essential oil and methanol extract of Echinophora platyloba D.C against some of food-borne pathogenic bacteria. Vet Res Forum 2013; 4(2): 123-127.
  5. Beigomi M, Mohsenzadeh M, Salari A. Characterization of a novel biodegradable edible film obtained from Dracocephalum moldavica seed mucilage. Int J Biol Macromol 2018; 108: 874-883.
  6. Mehdizadeh T, Tajik H, Razavi Rohani SM, et al. Antibacterial, antioxidant and optical properties of edible starch-chitosan composite film containing Thymus kotschyanus essential oil. Vet Res Forum 2012; 3(3): 167-173.
  7. Snoussi M, Noumi E, Punchappady-Devasya R, et al. Antioxidant properties and anti-quorum sensing potential of Carum copticum essential oil and phenolics against Chromobacterium violaceum. J Food Sci Technol 2018; 55(8): 2824-2832.
  8. Xu T, Gao C, Yang Y, et al. Retention and release properties of cinnamon essential oil in antimicrobial films based on chitosan and gum arabic. Food Hydrocoll 2018; 84: 84-92. ‏
  9. Khalil N, Ashour M, Fikry S, et al. Chemical composition and antimicrobial activity of the essential oils of selected Apiaceous fruits. Future J Pharm Sci 2018; 4(1): 88-92. ‏
  10. Patsilinakos A, Artini M, Papa R, et al. Machine learning analyses on data including essential oil chemical composition and in Vitro experimental antibiofilm activities against Staphylococcus Molecules 2019; 24(5): 890. ‏ doi: 10.3390/molecules24050890.
  11. Mimica-Dukić N, Bugarin D, Grbović S, et al. Essential oil of Myrtus communis as a potential antioxidant and antimutagenic agents. Molecules 2010; 15(4): 2759-2770.‏
  12. Aktaş B, Özdemir P, Basmacıoğlu-Malayoğlu H. In vitro antioxidant activities, total phenolic contents and main phenolic compounds of essential oil blend and grape seed extract. J Anim Prod 2018; 59(2): 43-47. ‏
  13. Khajeh M, Yamini Y, Sefidkon F, et al. Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydro distillation methods. Food Chem 2004; 86(4): 587-591.
  14. Soltani Howyzeh M, Sadat Noori SA, Shariati JV, et al. Essential oil chemotype of Iranian ajowan (Trachyspermum ammi L.). J. Essent Oil-Bear Plants 2018; 21(1): 273-276. ‏
  15. Mahmoudzadeh M, Hosseini H, Nasrollahzadeh J, et al. Antibacterial activity of Carum copticum essential oil against Escherichia coli O157:H7 in meat: Stx genes expression. Curr Microbiol 2016; 73(2): 265-272.
  16. Rabiey S, Hosseini H, Rezaei M. Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system. Braz J Microbiol 2014; 45(1): 89-96.
  17. Radoshevich L, Cossart P. Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis. Nat Rev Microbiol 2018; 16(1): 32-46. ‏
  18. Reis PMCL, Mezzomo N, Aguiar GPS, et al. Ultrasound-assisted emulsion of laurel leaves essential oil (Laurus nobilis ) encapsulated by SFEE. J Supercrit Fluid 2019; 147: 284-292. ‏
  19. Winkelmeyer CB, Peyronel F, Weiss J, et al. Monitoring tempered dark chocolate using ultrasonic spectro-metry. Food Bioprocess Tech 2016; 9(10): 1692-1705.
  20. Kentish S, Wooster TJ, Ashokkumar A, et al. The use of ultrasonics for nanoemulsion preparation. Innov Food Sci Emerg Technol 2008; 9(2): 170-175.
  21. Ostertag F, Weiss J, McClements DJ. Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. J Colloid Interface Sci 2012; 388(1): 95-102.
  22. Kahkha MRR, Amanloo S, Kaykhaii M. Antiaflatoxigenic activity of Carum copticum essential oil. Environ Chem Lett 2014; 12(1): 231-234.‏
  23. Jebelli Javan A, Bolandi M, Jadidi Z, et al. Effects of Scrophularia striata water extract on quality and shelf life of rainbow trout (Oncorhynchus mykiss) fillets during superchilled storage. Iran J Vet Res 2015; 16(2): 213-217.
  24. Kirby BJ. Micro- and nanoscale fluid mechanics: transport in microfluidic devices. New York, USA: Cambridge University Press 2010; 225-249.
  25. Hanaor DAH, Michelazzi M, Leonelli C, et al. The effects of carboxylic acids on the aqueous dispersion and electrophoretic deposition of ZrO2. J Eur Ceram Soc 2012; 32(1): 235-244.
  26. Kamkar A, Javan AJ, Asadi F, et al. The antioxidative effect of Iranian Mentha pulegium extracts and essential oil in sunflower oil. Food Chem Toxicol 2010; 48(7): 1796-1800.
  27. Tajik H, Naghili H, Ghasemmahdi H, et al. Effects of Zataria multiflora boiss essential oil, ultraviolet radiation and their combination on Listeria monocytogenes biofilm in a simulated industrial model. Int J Food Sci Technol 2015; 50(9): 2113-2119.
  28. Becerril R, Gómez-Lus R, Goñi P, et al. Combination of analytical and microbiological techniques to study the antimicrobial activity of a new active food packaging containing cinnamon or oregano against coli and S. aureus. Anal Bioanal Chem 2007; 388(5-6): 1003-1011.
  29. Shahabi N, Tajik H, Moradi M, et al. Physical, antimicrobial and antibiofilm properties of Zataria multiflora boiss essential oil nanoemulsion. Int J Food Sci Technol 2017; 52(7): 1645-1652. ‏
  30. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 2016; 6(2): 71-79.
  31. Konaté K, Mavoungou JF, Lepengué AN, et al. Antibacterial activity against β-lactamase producing methicillin and ampicillin-resistants Staphylococcus aureus: fractional inhibitory concentration index (FICI) determination. Annals of clinical microbiology and antimicrobials. Ann Clin Microbiol Antimicrob 2012; 11:18. doi: 10.1186/1476-0711-11-18.
  32. Djordjevic D, Wiedmann M, McLandsborough LA. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl Environ Microbiol 2002; 68(6): 2950-2958.
  33. Kavoosi G, Tafsiry A, Ebdam AA, et al. Evaluation of antioxidant and antimicrobial activities of essential oils from Carum copticum seed and Ferula assafoetida latex. J Food Sci 2013; 78(2): T356-T361. ‏
  34. Mohammadpour G, Tahmasbpour R, Rahmani A, et al. Chemical compounds, in vitro antitumor and antibacterial activities of Trachyspermum copticum L essential oil. Iran J Pharmacol Ther 2018; 16(1):1-6. ‏
  35. Hashemi Gahruie H, Ziaee E, Eskandari MH, et al. Characterization of basil seed gum-based edible films incorporated with Zataria multiflora essential oil nanoemulsion. Carbohydr Polym 2017; 166: 93-103. ‏
  36. Masoomi V, Tajik H, Moradi M, et al. Antimicrobial effects of Zataria multiflora boiss essential oil nanoemulsion against coli O157: H7 [Persian]. Urmia Med J 2016; 27(7): 608-617.
  37. Esmaeili A, Asgari A. In vitro release and biological activities of Carum copticum essential oil (CEO) loaded chitosan nanoparticles. Int J Biol Macromol 2015; 81: 283-290.
  38. Hashemi MB, Niakousari M, Saharkhiz MJ, et al. Stabilization of sunflower oil with Carum copticum Benth and Hook essential oil. J Food Sci Technol 2014; 51(1): 142-147. ‏
  39. Nickavar B, Adeli A, Nickavar A. TLC-bioautography and GC-MS analyses for detection and identification of antioxidant constituents of Trachyspermum copticum essential oil. Iran J Pharm Res 2014; 13(1): 127-133.‏
  40. Katalinic V, Milos M, Kulisic T, et al. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 2006; 94(4): 550-557.
  41. Thériault M, Caillet S, Kermasha S, et al. Antioxidant, antiradical and antimutagenic activities of phenolic compounds present in maple products. Food Chem 2006; 98(3): 490-501.
  42. Bhargava K, Conti DS, da Rocha SR, et al. Application of an oregano oil nanoemulsion to the control of food-borne bacteria on fresh lettuce. Food Microbiol 2015; 47: 69-73.
  43. Donsì F, Annunziata M, Sessa M, et al. Nano-encapsulation of essential oils to enhance their antimicrobial activity in foods. LWT-Food Sci Technol 2011; 44(9): 1908-1914.
  44. Kazemi Oskuee R, Behravan J, Ramezani M. Chemical composition, antimicrobial activity and antiviral activity of essential oil of Carum copticum from Iran. Avicenna J Phytomed 2011; 1(2): 83-90.
  45. Moghimi R, Ghaderi L, Rafati H, et al. Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against coli. Food Chem 2016; 194: 410-415.
  46. Topuz OK, Özvural EB, Zhao Q, et al. Physical and antimicrobial properties of anise oil loaded nanoemulsions on the survival of foodborne pathogens. Food Chem 2016; 203: 117-123.
  47. Zomorodian K, Ghadiri P, Saharkhiz MJ, et al. Antimicrobial activity of seven essential oils from Iranian aromatic plants against common causes of oral infections. Jundishapur J Microbiol 2015; 8(2): e17766.‏ doi: 10.5812/jjm.17766
  48. Li YF, Sun HW, Gao R, et al. Inhibited biofilm formation and improved antibacterial activity of a novel nanoemulsion against cariogenic Streptococcus mutans in vitro and in vivo. Int J Nanomedicine 2015; 10: 447-462.
Veterinary Research Forum
Volume 12, Issue 4
December 2021
Pages 437-444
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History
  • Receive Date: 07 September 2019
  • Revise Date: 16 November 2019
  • Accept Date: 08 January 2020
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