Pulsed-field gel electrophoresis fingerprinting of Listeria monocytogenes ‎isolates recovered from foods of animal origin and fishes in North-Eastern ‎India

Document Type : Short Communication

Authors

1 Division of Animal Health, Indian Council of Agricultural Research (ICAR) Research Complex for North Eastern Hill Region, Umiam, India‎

2 Department of Microbiology, Goa University, Taleigaon Plateau, India

3 ICAR-National Research Centre on Meat, Hyderabad, India

Abstract

Listeria monocytogenes is a pathogen of great concern to the food industry. The present study was aimed to explore the clonal relationships amongst L. monocytogenes strains isolated from foods of animal origin (milk, beef, chevon (goat meat), pork and chicken) and fish. Forty-six L. monocytogenes strains were characterized by pulsed-field gel electrophoresis (PFGE). The PFGE analysis using ApaI and AscI enzymes revealed 37 pulsotypes, with Simpson’s discriminatory index of 0.987. This study demonstrated the presence of a few similar L. monocytogenes pulsotypes in different foods of animal origin in different places and years of isolation and this indicates that some L. monocytogenes subtypes may be ubiquitous which are acclimatizing and persisting in different foods of animal origin. This also emphasizes the importance of cross-contamination in local wet markets. Thus, the understanding of genetic diversity will contribute to the development of rational and workable strategies to control this important zoonotic infection. 

Keywords


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Volume 13, Issue 1
March 2022
Pages 133-139
  • Receive Date: 15 February 2020
  • Revise Date: 30 June 2020
  • Accept Date: 03 August 2020
  • First Publish Date: 15 December 2021