Shiga toxin-producing Escherichia coli O157 in piglets and food from backyard systems

Document Type : Original Article

Authors

1 Department of Microbiology, Faculty of Veterinary Medicine and Zootechnics, Autonomous University of Chiapas, Chiapas, Mexico

2 Faculty of Agronomic Sciences, Autonomous University of Chiapas, Chiapas, Mexico‎

3 Institute of Biological Sciences, University of Sciences and Arts of Chiapas, Chiapas, Mexico‎

4 Faculty of Veterinary Medicine and Zootechnics, Autonomous University of Chiapas, Chiapas, Mexico

5 Institute of Agricultural Sciences, Autonomous University of Baja California, Baja California, Mexico

Abstract

Piglets suffer from diarrhea caused by the Shiga toxin-producing Escherichia coli (STEC) and can be carriers of the bacteria, with public health consequences in developing countries. The aim of the present study was to study the prevalence of STEC O157 in feces of 465 piglets and 54 food mixes from backyard systems, the antimicrobial susceptibility of STEC and the frequency of genes encoding extended-spectrum β-lactamases. The E. coli was isolated from 75.90 % of the evaluated feces. The STEC strains were identified in 33.11% of the sampled population and in 43.60% of the piglets carrying E. coli. Among STEC strains, the stx1 gene was the most frequent (22.30%). The rfbO157 gene was amplified in 47.40% of the STEC strains. High frequencies of STEC strains were not susceptible to ampicillin, carbenicillin and tetracycline. The blaTEM gene (52) was the most frequent among strains not susceptible to ampicillin. Class 1 integrons were the most frequent in those strains. Of the identified STEC strains, 48.70% were considered as multi-drug resistant and 1.90% were considered extensively drug resistant. In the supplied food, STEC O157 strains were identified in 25.00% of the STEC strains. We conclude that the piglets from backyard systems are carriers of STEC O157 strains not susceptible to common antibiotics, including penicillins and tetracyclines. In addition, supplied food is a source of this type of pathogenic bacteria. Through their direct contact with humans, the piglets and food represent a potential source of bacterial dissemination capable of producing gastrointestinal infections in humans.

Keywords

References

  1. Heo JM, Opapeju FO, Pluske J R, et al. Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post‐weaning diarrhoea without using in‐feed antimicrobial compounds. J Anim Physiol Amim Nutr (Berl) 2013; 97(2): 207-237.
  2. Malgarin CM, Takeuti KL, de Lara AC, et al. Virulence factors and antimicrobial resistance profile of Escherichia coli isolated from nursery piglets and drinking water. Acta Sci Vet 2018; 46(1): 6. doi:10.22456/1679-9216.81810.
  3. Yang GY, Guo L, Su JH, et al. Frequency of diarrheagenic virulence genes and characteristics in Escherichia coli isolates from pigs with diarrhea in china. Micro-organisms 2019; 7(9): 308. doi:10.3390/microorganisms7090308.
  4. Ibama O, Ibegbulem EO, Onwuli D, et al. The health implication of enterohaemorrhagic Escherichia coli (EHEC) 0157: H7: A review on haemolytic uraemic syndrome. AJRIMPS 2019; 7(4): 1-10.
  5. Ungureanu V, Corcionivoschi N, Gundogdu O, et al. The emergence of beta-lactamase producing Escherichia coli and the problems in assessing their potential contribution to foodborne illness: a review. AgroLife Sci J 2019; 8(1): 248-260.
  6. Smith MG, Jordan D, Gibson JS, et al. Phenotypic and genotypic profiling of antimicrobial resistance in enteric Escherichia coli communities isolated from finisher pigs in Australia. Aust Vet J 2016; 94(10): 371-376.
  7. Toledo A, Gómez D, Cruz C, et al. Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico. J Med Microbiol 2012; 61(Pt 1): 148-156.
  8. Callaway TR, Anderson RC, Tellez G, et al. Prevalence of Escherichia coli O157 in cattle and swine in central Mexico. J Food Prot 2004; 67(10): 2274-2276.
  9. Montero-López EM, Martínez-Gamba RG, Herradora-Lozano MA, et al. Alternatives for small-scale pig production [Spanish]. Coyoacan, Mexico: National Autonomous University of Mexico. 2015; 17-35.
  10. Cerna-Cortés JF, Gómez-Aldapa CA, Rangel-Vargas E, et al. Presence of indicator bacteria, Salmonella and diarrheagenic coli pathotypes on mung bean sprouts from public markets in Pachuca, Mexico. Food Control 2013; 31(2): 280-283.
  11. Tsai YL, Palmer CJ, Sangermano LR. Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Appl Environ Microbiol 1993; 59(2): 353-357.
  12. Tarr PI, Gordon CA, Chandler WL. Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. Lancet 2005; 365(9464): 1073-1086.
  13. Gutiérrez-Jiménez J, Luna-Cazárez LM, Mendoza-Orozco MI, et al. Organization, maintenance, and preservation of the bacterial culture collection of the biological sciences institute, university of science and arts of Chiapas (UNICACH), Mexico [Spanish]. Rev Soc Ven Microbiol 2015; 35(2): 95-102.
  14. López-Saucedo C, Cerna JF, Villegas-Sepulveda N, et al. Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli. Emerg Infect Dis 2003; 9(1): 127-131.
  15. Paton AW, Paton JC. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic coli hlyA, rfbO111, and rfbO157. J Clin Microbiol 1998; 36(2): 598-602.
  16. Ahmed AM, Motoi Y, Sato M, et al. Zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes. Appl Environ Microbiol 2007; 73(20): 6686-6690.
  17. Mazel D, Dychinco B, Webb VA. Antibiotic resistance in the ECOR collection: integrons and identification of a novel aad gene. Antimicrob Agents Chemother 2000; 44(6): 1568-1574.
  18. White PA, McIver CJ, Rawlinson WD. Integrons and gene cassettes in the enterobacteriaceae. Antimicrob Agents Chemother 2001; 45(9): 2658-2661.
  19. Wayne PA. Performance standards for antimicrobial susceptibility testing; 24th informational supplement. Document M100-S24 Clinical and laboratory standards institute (CLSI) 2014: 50,110.
  20. Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012; 18(3): 268-281.
  21. Saavedra‐Montañez M, Carrera‐Aguirre V, Castillo‐Juárez H, et al. Retrospective serological survey of influenza viruses in backyard pigs from Mexico City. Influenza Other Respir Viruses 2013; 7(5): 827-832.
  22. Chen L, Wang L, Yassin AK, et al. Genetic characterization of extraintestinal Escherichia coli isolates from chicken, cow and swine. AMB Express 2018; 8(1): 117. doi:10.1186/s13568-018-0646-8.
  23. Bautista‐De León H, Gómez‐Aldapa CA, Rangel‐Vargas E, et al. Frequency of indicator bacteria, Salmonella and diarrhoeagenic Escherichia coli pathotypes on ready‐to‐eat cooked vegetable salads from Mexican restaurants. Lett Appl Microbiol 2013; 56(6): 414-420.
  24. Gómez-Aldapa CA, Torres-Vitela Mdel R, Acevedo-Sandoval OA, et al. Presence of Shiga toxin–producing Escherichia coli, enteroinvasive coli, enteropathogenic E. coli, and enterotoxigenic E. coli on tomatoes from public markets in Mexico. J Food Prot 2013; 76(9): 1621-1625.
  25. Paredes‐Paredes M, Okhuysen PC, Flores J, et al. Seasonality of diarrheagenic Escherichia coli pathotypes in the US students acquiring diarrhea in Mexico. J Travel Med 2011; 18(2): 121-125.
  26. Canizalez-Roman A, Flores-Villaseñor HM, Gonzalez-Nuñez E, et al. Surveillance of diarrheagenic Escherichia coli strains isolated from diarrhea cases from children, adults and elderly at Northwest of Mexico. Front Microbiol 2016; 7: 1924. doi:10.3389/fmicb. 2016.01924.
  27. Amézquita-López BA, Quiñones B, Soto-Beltrán M, et al. Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and Non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico. Antimicrob Resist Infect Control 2016; 5: 1. doi:10.1186/s13756-015-0100-5.
  28. Hasan MS, Yousif A, Alwan MJ. Detection of virulent genes in coli O157: H7 isolated from puppies and adult dogs by polymerase chain reaction. Res J Vet Pract 2016; 4(1): 1-6.
  29. Meng Q, Bai X, Zhao A, et al. Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China. BMC Microbiol 2014; 14: 5. doi:10.1186/1471-2180-14-5.
  30. Buranasinsup S, Kulpeanprasit S, Kong-ngoen T, et al. Prevalence of the multi-drug resistance of Shiga toxin-producing Escherichia coli isolated from pigs in central Thailand. Chiang Mai J Sci 2018; 45(1): 21-32.
  31. Samanta I, Joardar SN, Mahanti A, et al. Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India. Infect Genet Evol 2015; 36: 224-230.
  32. Changkaew K, Intarapuk A, Utrarachkij F, et al. Antimicrobial resistance, extended-spectrum β-lactamase productivity, and class 1 integrons in Escherichia coli from healthy swine. J Food Prot 2015; 78(8): 1442-1450.
  33. Delmani FA, Jaran AS, Al Tarazi Y, et al. Characterization of ampicillin resistant gene (blaTEM-1) isolated from coli in Northern Jordan. Asian J Biomed Pharmaceut Sci 2017; 7(61): 11-15.
  34. Nagachinta S, Chen J. Integron-mediated antibiotic resistance in Shiga toxin-producing Escherichia coli. J Food Prot 2009; 72(1): 21-27.
Veterinary Research Forum
Volume 13, Issue 2
June 2022
Pages 169-176

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History

  • Receive Date: 07 June 2020
  • Accept Date: 03 November 2020
  • First Publish Date: 05 April 2022

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