Estimation of gestational age using ultrasonography in Baluchi sheep

Document Type : Original Article

Authors

1 DVM Graduate, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

The aim of this study was to evaluate the correlation between some fetal parameters measured by ultrasonography and fetal age for developing a formula which could be used for estimation of fetal age in Baluchi sheep. Placentome diameter (PD), gestation Sac diameter (GSD), biparietal diameter (BPD), straight crown-rump length (CRL), femural length (FL), occipito-snout diameter (OSD), thoracic diameter (TD) and fetal heart rate (FHR) were measured by transrectal and/or transabdominal ultrasonography. All ewes were synchronized using progesterone sponge for 6 days. On the first day, they received PGF2 and on the last day received 400 IU of equine serum gonadotrophin (eCG). All of the parameters revealed significant correlation by increasing fetal age. Except for PD, for other values linear regression curve was illustrated. BPD (between 36 - 96 days) (R2 = 0.961), CRL (R2 = 0.935), FL (R2 = 0.950), OSD (R2 = 0.981), TD (R2 = 0.975) showed high correlation with fetal age (p < 0.001). Moderate correlation was calculated for FHR (p < 0.001, R2 = 0.883). Low correlations were assessed by measurement of GSD (p = 0.018, R2 = 0.318), BPD (between 96-138 days; p = 0.038, R2 = 0.29) and PD represented the significant non-linear correlation with age (p < 0.001), maximum correlation was assessed by measurement of mean placentome wall diameter. In conclusion, OSD was recommended as the best parameter for estimating of gestational age between days 36 - 109 in Baluchi sheep because of the lowest residuals, the highest correlation coefficient and wide period of availability for imaging in gestation.

Keywords

References

  1. Doizé F, Vaillancourt D, Carabin H, et al. Determination of gestational age in sheep and goats using transrectal ultrasonographic measurement of placentomes. Theriogenology 1997; 48(3): 449-460.
  2. Fthenakis GC, Arsenos G, Brozos C, et al. Health management of ewes during pregnancy. Anim Reprod Sci 2012; 130(3): 198-212.
  3. Ishwar AK. Pregnancy diagnosis in sheep and goats: a review. Small Rumin Res 1995; 17(1): 37-44.
  4. Gonzalez‐Bulnes A, Pallares P, Vazquez MI. Ultrasonographic imaging in small ruminant reproduction. Reprod Domest Anim 2010; 45(Suppl 2): 9-20.
  5. Fraser AF, Robertson JG. Pregnancy diagnosis and detection of foetal life in sheep and pigs by an ultrasonic method. Br Vet J 1968; 124(6): 239-244.
  6. Lindhal IL. Pregnancy diagnosis in ewes by ultrasonic scanning. J Anim Sci 1976; 43(6): 1135–1140.
  7. Lindahl IL. Pregnancy diagnosis in the ewe by intrarectal Doppler. J Anim Sci 1971; 32(5): 922-925.
  8. Ali A, Hayder M. Ultrasonographic assessment of embryonic, fetal and placental development in Ossimi sheep. Small Rumin Res 2007; 73: 277-282.
  9. de Bulnes AG, Moreno JS, Sebastián AL. Estimation of fetal development in Manchega dairy ewes by transrectal ultrasonographic measurements. Small Rumin Res 1998; 27(3): 243-250.
  10. Gearhart MA, Wingfield WE, Knight AP, et al. Real-time ultrasonography for determining pregnancy status and viable fetal numbers in ewes. Theriogenology 1988; 30(2): 323-337.
  11. Godfrey RW, Larson L, Weis AJ, et al. Evaluation of ultrasonography to measure fetal size and heart rate as predictors of fetal age in hair sheep. Sheep Goat Res J; 2010. 25: 60-65.
  12. Greenwood PL, Slepetis RM, McPhee MJ, et al. Prediction of stage of pregnancy in prolific sheep using ultrasound measurement of fetal bones. Reprod Fertil Dev 2002: 14(1-2): 7-13.
  13. Gunduz MC, Turna O, Ucmak M, et al. Prediction of gestational week in Kivircik ewes using fetal ultrasound measurements. Agric J 2010; 5(2): 110-115.
  14. Haibel GK, Perkins NR. Real-time ultrasonic biparietal diameter of second trimester Suffolk and Finn sheep fetuses and prediction of gestational age. Theriogenology 1989; 32(5): 863-869.
  15. Haibel GK. Use of ultrasonography in reproductive management of sheep and goat herds. Vet Clin North Am: Food Anim Pract 1990; 6(3): 597-613.
  16. Jones AK, Gately RE, McFadden KK, et al. Ultrasound during mid‐gestation: Agreement with physical foetal and placental measurements and use in predicting gestational age in sheep. Reprod Domest Anim 2017; 52(4): 649-654.
  17. Jones AK, Gately RE, McFadden KK, et al. Trans-abdominal ultrasound for detection of pregnancy, fetal and placental landmarks, and fetal age before day 45 of gestation in the sheep. Theriogenology 2016; 85(5): 939-945.
  18. Kelly RW, Newnham JP. Estimation of gestational age in Merino ewes by ultrasound measurement of fetal head size. Aust J Agric Res 1989; 40(6): 1293-1299.
  19. Petrujkić BT, Cojkić A, Petrujkić K, et al. Trans-abdominal and transrectal ultrasonography of fetuses in Württemberg ewes: Correlation with gestational age. Anim Sci J 2016; 87(2): 197-201.
  20. Santiago-Moreno J, González-Bulnes A, Gómez-Brunet A, et al. Prediction of gestational age by trans-rectal ultrasonographic measurements in the mouflon (Ovis gmelini musimon). J Zoo Wildl Med 2005; 36(3): 457-462.
  21. Correia Santos VJ, Garcia Kako Rodriguez M, da Del Aguila da Silva P, et al. B-mode ultrasonography and ecobiometric parameters for assessment of embryonic and fetal development in sheep. Anim Reprod Sci 2018; 197: 193-202.
  22. Sergeev L, Kleemann DO, Walker SK, et al. Real-time ultrasound imaging for predicting ovine fetal age. Theriogenology 1990; 34(3): 593-601.
  23. Soroori S, Veshkini A, Tajik P. Assessment of the gestational age in Chall fetus by measuring the embryonic vesicle, biparietal diameter, and crown ramp length. J Vet Res 2007; 62(2): 1-5.
  24. Waziri MA, Ikpe AB, Bukar MM, et al. Determination of gestational age through trans-abdominal scan of placentome diameter in Nigerian breed of sheep and goats. Sokoto J Vet Sci 2017: 15(2): 49-53.
  25. Aiumlamai S, Fredriksson G, Nilsfors L. Real-time ultrasonography for determining the gestational age of ewes. Vet Rec 1992; 131(24): 560-562.
  26. Kandiel MMM, Watanabe G, Taya K. Ultrasonographic assessment of fetal growth in miniature “Shiba” goats (Capra hircus). Anim Reprod Sci 2015: 162: 1-10.
  27. Tahmoorespur M, Sheikhloo M. Pedigree analysis of the closed nucleus of Iranian Baluchi sheep. Small Rumin Res 2011; 99(1): 1-6.
  28. Schrick FN, Inskeep EK, Determination of early pregnancy in ewes utilizing transrectal ultras-onography. Theriogenology 1993; 40(2): 295-306.
  29. Kaulfuss KH, Zipper N, May J, et al. Ultrasonic pregnancy diagnosis (B-mode) in sheep. 2. Comparative studies using transcutaneous and transrectal pregnancy diagnosis [German]. Tierarztl Prax 1996; 24(6): 559-566.
  30. Buckrell BC, Bonnett BN, Johnson WH. The use of real-time ultrasound rectally for early pregnancy diagnosis in sheep. Theriogenology 1986; 25(5): 665-673.
  31. Chavez Moreno J, Steinmann Chavez C, Bickhardt K. Fetal heart rate measurement and sonographic fetometry for determination of fetal age in sheep. [German] Dtsch Tierarztl Wochenschr1996; 103(11): 478-480.
  32. Metodiev N, Dimov D, Ralchev I, et al. Measurements of foetal growth via transabdominal ultrasonography during first half of pregnancy at ewes from synthetic population Bulgarian milk. Bulg J Agric Sci 2012; 18(4): 493-500.
  33. Stankiewicz T, Błaszczyk B, Udała J, et al Morpho-metric measurements of the umbilical cord and placentomes and Doppler parameters of the umbilical artery through ultrasonographic analysis in pregnant sheep. Small Rumin Res 2020; 184: 106043. doi: 10.1016/j.smallrumres.2019.106043.
  34. Barbera A, Jones WO, Zerbe GO, et al. Ultrasonographic assessment of fetal growth: Comparison between human and ovine fetus. Am J Obstet Gynecol 1995; 173(6): 1765-1769.
Veterinary Research Forum
Volume 13, Issue 2
June 2022
Pages 257-263

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History

  • Receive Date: 28 July 2020
  • Revise Date: 08 February 2021
  • Accept Date: 01 March 2021
  • First Publish Date: 05 April 2022

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