Veterinary Research Forum

Vet Res Forum

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Effect of chlorogenic acid on follicular development, hormonal status and biomarkers of oxidative stress in rats with polycystic ovary syndrome

Document Type : Original Article

Authors

1 Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2 Student Research Committee, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Abstract

Polycystic ovarian syndrome (PCOS) is a complex endocrine and metabolic disorder. Chlorogenic acid (CGA) bears antioxidant properties with protective effects on different tissues. This study was conducted to evaluate the effect of CGA on follicular development, hormonal status and biomarkers of oxidative stress in a rat model of PCOS. In this experimental study, 18 rats were divided into three equal groups including: control, non-treated PCOS [(estradiol valerate (EV): 40.00 mg kg-1 intramuscularly)], and PCOS-CGA (EV: 40.00 mg kg-1 intramuscularly and CGA: 100 mg kg-1 intraperitoneally once a week for eight consecutive weeks). At the end of treatment period, all rats were anesthetized. Then 5.00 mL blood samples of rats in the three groups were taken and prepared for hormonal analyses and their ovaries were isolated and dissected mechanically free of fat and mesentery. The ovaries underwent the following analyses: Morphological study with Hematoxylin and Eosin staining and biochemical study using the malondialdehyde (MDA) level and total antioxidant activity. Data were analyzed using one-way ANOVA and post hoc Tukey’s test. The serum level of luteinizing hormone, estrogen, testosterone, antioxidant capacity, glutathione and the number of cystic follicles in the PCOS group treated with 100 mg kg-1 Chlorogenic acid compared to the non-treated PCOS group were significantly decreased, however, the serum level of follicle stimulating hormone, progesterone, MDA and the number of secondary, graafian follicles and corpus luteum were significantly increased. Chlorogenic acid could be effective in ameliorating follicular development as well as hormonal and biochemical disorders in rats with PCOS.

Keywords

References
  1. Stener-Victorin E, Padmanabhan V, Walters KA, et al. Animal models to understand the etiology and patho-physiology of polycystic ovary syndrome. Endocr Rev 2020; 41(4): bnaa010. doi: 10.1210/ endrev/bnaa010.
  2. Tehrani FR, Simbar M, Tohidi M, et al. The prevalence of polycystic ovary syndrome in a community sample of Iranian population: Iranian PCOS prevalence study. Reprod Biol Endocrinol 2011; 9: 39. doi: 10.1186/ 1477-7827-9-39.
  3. Setji TL, Brown AJ. Polycystic ovary syndrome: update on diagnosis and treatment. Am J Med 2014; 127(10): 912-919.
  4. Lewandowski KC, Cajdler-Łuba A, Salata I, et al. The utility of the gonadotrophin releasing hormone (GnRH) test in the diagnosis of polycystic ovary syndrome (PCOS). Endokrynol Pol 2011; 62(2): 120-128.
  5. Shi Y, Kong X, Yin H, et al. Effect of hawthorn leaf flavonoids in dehydroepiandrosterone-induced poly-cystic ovary syndrome in rats. Pathobiology 2019; 86(2-3): 102-110.
  6. Weiss NS, Kostova E, Nahuis M, et al. Gonadotrophins for ovulation induction in women with polycystic ovary syndrome. Cochrane Database Syst Rev 2019; 16: (1). CD010290. doi: 10.1002/ 14651858. CD010290.pub3.
  7. Mohammadi M. Oxidative stress and polycystic ovary syndrome: a brief review. Int J Prev Med 2019; 10: 86. doi: 10.4103/ijpvm.IJPVM_576_17.
  8. Ekici K, Temelli O, Parlakpinar H, et al. Beneficial effects of aminoguanidine on radiotherapy-induced kidney and testis injury. Andrologia 2016; 48(6): 683-692.
  9. Murri M, Luque-Ramírez M, Insenser M, et al. Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): a systematic review and meta-analysis. Hum Reprod Update 2013; 19(3): 268-288.
  10. Blair SA, Kyaw-Tun T, Young IS, et al. Oxidative stress and inflammation in lean and obese subjects with polycystic ovary syndrome. J Reprod Med 2013; 58(3-4): 107-114.
  11. Furat Rencber S, Kurnaz Ozbek S, Eraldemır C, et al. Effect of resveratrol and metformin on ovarian reserve and ultrastructure in PCOS: an experimental study. J Ovarian Res 2018; 11(1): 55. doi: 10.1186/s13048-018-0427-7.
  12. Chen J, Guo Q, Pei YH, et al. Effect of a short-term vitamin E supplementation on oxidative stress in infertile PCOS women under ovulation induction: a retrospective cohort study. BMC Womens Health 2020; 20(1): 69. doi: 10.1186/s12905-020-00930-w.
  13. Wu G, Hu X, Ding J, et al. The effect of glutamine on dehydroepiandrosterone-induced polycystic ovary syndrome rats. J Ovarian Res 2020; 13: 57. doi: 10.1186/s13048-020-00650-7.
  14. Surai PF, Kochish II, Fisinin VI, et al. Antioxidant defence systems and oxidative stress in poultry biology: an update. Antioxidants (Basel) 2019; 8(7): 235. doi: 10.3390/antiox8070235.
  15. Tajik N, Tajik M, Mack I, et al. The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature. Eur J Nutr 2017; 56(7): 2215-2244.
  16. Nguyen TV, Wittayarat M, Do LTK, et al. Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro Anim Sci J 2018; 89(8): 1207-1213.
  17. Nguyen TV, Tanihara F, Do L, et al. Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes. Reprod Domest Anim 2017; 52(6): 969-975.
  18. Javanmard MZ, Meghrazi K, Ghafori SS, et al. The ameliorating effects of Vitamin E on hepatotoxicity of ecstasy. J Res Med Sci. 2020; 25: 91. doi: 10.4103/ jrms.JRMS_496_19.
  19. Brawer JR, Munoz M, Farookhi R. Development of the polycystic ovarian condition (PCO) in the estradiol valerate-treated rat. Biol Reprod 1986; 35(3): 647-655.
  20. Beloosesky R, Gold R, Almog B, et al. Induction of polycystic ovary by testosterone in immature female rats: Modulation of apoptosis and attenuation of glucose/insulin ratio. Int J Mol Med 2004; 14(2): 207-215.
  21. Arentz S, Smith CA, Abbott J, et al. Combined lifestyle and herbal medicine in overweight women with poly-cystic ovary syndrome (PCOS): a randomized controlled trial. Phytother Res 2017; 31(9): 1330-1340.
  22. Watanabe T, Kobayashi S, Yamaguchi T, et al. Coffee abundant in chlorogenic acids reduces abdominal fat in overweight adults: a randomized, double-blind, controlled trial. Nutrients 2019; 11(7): 1617. doi: 10.3390/nu11071617.
  23. Sun L, Ji C, Jin L, et al. Effects of exenatide on metabolic changes, sexual hormones, inflammatory cytokines, adipokines, and weight change in a DHEA-treated rat model. Reprod Sci 2016; 23(9): 1242-1249.
  24. Ghafurniyan H, Azarnia M, Nabiuni M, et al. The effect of green tea extract on reproductive improve-ment in estradiol valerate-induced polycystic ovarian syndrome in rat. Iran J Pharm Res 2015; 14(4): 1215-1233.
  25. Guan Y, Wang D, Bu H, et al. The effect of metformin on polycystic ovary syndrome in overweight women: a systematic review and meta-analysis of randomized controlled trials. Int J Endocrinol 2020; 2020: 5150684. doi: 10.1155/2020/5150684.
  26. Naseri L, Khazaei MR, Khazaei M. Potential therapeutic effect of bee pollen and metformin combination on testosterone and estradiol levels, apoptotic markers and total antioxidant capacity in a rat model of polycystic ovary syndrome. Int J Fertil Steril 2021; 15(2): 101-107.
  27. Tahmasebi F, Movahedin M, Mazaheri Z. Antioxidant effects of calligonum extract on ovarian tissue of PCO model: An experimental study. Int J Reprod Biomed 2018; 16(10): 641-648.
  28. Matsuda F, Inoue N, Manabe N, et al. Follicular growth and atresia in mammalian ovaries: regulation by survival and death of granulosa cells. J Reprod Dev 2012; 58(1): 44-50.
  29. Ergenoglu M, Yildirim N, Yildirim AG, et al. Effects of resveratrol on ovarian morphology, plasma anti-mullerian hormone, IGF-1 levels, and oxidative stress parameters in a rat model of polycystic ovary syndrome. Reprod Sci 2015; 22(8): 942-947.
  30. Grossman MP, Nakajima ST, Fallat ME, et al. Müllerian-inhibiting substance inhibits cytochrome P450 aromatase activity in human granulosa lutein cell culture. Fertil Steril 2008; 89(5 Suppl): 1364-1370.
  31. Chen WP, Wu LD. Chlorogenic acid suppresses interleukin-1β-induced inflammatory mediators in human chondrocytes. Int J Clin Exp Pathol 2014; 7(12): 8797-8801.
  32. Grosser PM, McCarthy GF, Robaire B, et al. Plasma patterns of LH, FSH and prolactin in rats with a polycystic ovarian condition induced by oestradiol valerate. J Endocrinol 1987; 114(1): 33-39.
  33. Wan CW, Wong CN, Pin WK, et al. Chlorogenic acid exhibits cholesterol lowering and fatty liver attenuating properties by up-regulating the gene expression of PPAR-α in hypercholesterolemic rats induced with a high-cholesterol diet. Phytother Res 2013; 27(4): 545-551.
  34. Zhou RP, Lin SJ, Wan WB, et al. Chlorogenic acid prevents osteoporosis by Shp2/PI3K/Akt pathway in ovariectomized rats. PLoS One 2016; 11(12): e0166751. doi: 10.1371/journal.pone.0166751.
  35. Wang X, Wang GC, Rong J, et al. Identification of steroidogenic components derived from Gardenia jasminoides Ellis potentially useful for treating postmenopausal syndrome. Front Pharmacol 2018; 9: 390. doi: 10.3389/fphar.2018.00390.
  36. Mancini A, Bruno C, Vergani E, et al. Oxidative stress and low-grade inflammation in polycystic ovary syndrome: controversies and new insights. Int J Mol Sci 2021; 22(4): 1667. doi: 10.3390/ijms22041667.
  37. Dkhil MA, Abdel Moneim AE, Bauomy AA, et al. Chlorogenic acid prevents hepatotoxicity in arsenic-treated mice: role of oxidative stress and apoptosis. Mol Biol Rep 2020; 47(2): 1161-1171.
  38. Ali N, Rashid S, Nafees S, et al. Protective effect of Chlorogenic acid against methotrexate induced oxidative stress, inflammation and apoptosis in rat liver: An experimental approach Chem Biol Interact 2017; 272: 80-91.
  39. Santana-Gálvez J, Cisneros-Zevallos L, Jacobo-Velázquez DA. Chlorogenic acid: Recent advances on its dual role as a food additive and a nutraceutical against metabolic syndrome. Molecules 2017; 22(3): 358. doi: 10.3390/molecules22030358.
  40. Liu Y, He XQ, Huang X, et al. Resveratrol protects mouse oocytes from methylglyoxal-induced oxidative damage. PLoS One 2013; 8(10): e77960. doi: 10.1371/ journal. pone.0077960.
  41. Koriem KMM, Arbid MS. Role of caftaric acid in lead-associated nephrotoxicity in rats via antidiuretic, antioxidant and anti-apoptotic activities. J Complement Integr Med 2017; 15(2). doi: 10.1515/ jcim-2017-0024.
  42. Naveed M, Hejazi V, Abbas M, et al. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomed Pharmacother 2018; 97: 67-74.
Veterinary Research Forum
Volume 13, Issue 4
December 2022
Pages 513-520
Files
History
  • Receive Date: 17 May 2021
  • Revise Date: 10 July 2021
  • Accept Date: 13 September 2021
Share
How to cite
Statistics