Document Type : Original Article
Authors
Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
Abstract
Indigestible neutral detergent fibers (iNDF) accurately predict forage digestibility when measured in situ. The objective of this study was to determine the effects of rumen incubation times on the estimated concentrations of iNDF for four forages (alfalfa hay, corn silage, wheat straw and orchard grass), four concentrates (barley grain, soybean meal, beet pulp and wheat bran) and two total mixed ration samples in dairy cows. The iNDF contents of the samples were evaluated in 10 feeds using three ruminally cannulated Holstein cows in a completely randomized design. Five grams of the samples were incubated up to 240 hr. The iNDF fraction was significantly affected by incubation time for all of the tested samples, but the potentially digestible NDF fraction (pdNDF) was not affected for wheat straw, barley grain and wheat bran (32.32, 10.11 and 20.60 g per 100 g of dry matter, respectively). For most of concentrates feedstuffs, the iNDF fraction could be measured after 120 hr of incubation, while for forages ruminal incubation should be lasted up to 240 hr. Statistically significant differences (p < 0.01) were observed between forage samples regarding fragility and NDF digestibility (NDFD). Also, a positive correlation was observed between fragility and NDFD. In some of the cases, it appears that NDFD can be a more helpful index in adjusting pdNDF values than direct fragility measurements.
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- Cotanch KW, Grant RJ, Van Amburgh ME, et al. Applications of uNDF in ration modeling and formulation. New York, USA: Miner Agricultural Research Institute 2014;10-38.
- Zali SM, Teimouri Yansari A, Jafari Sayyadi A. Effect of particle size and fragility of corn silage and alfalfa hay on intake, digestibility, performance, and chewing activity of fattening male lambs. Res Rev J Vet Sci 2015; 1: 47-57.
- Allen M.S, Mertens DR. Evaluating constraints on fiber digestion by rumen microbes. J Nutr 1988; 118:
261-270. - Poppi DP, France J, McLennan SR. Intake, passage and digestibility. In: Theodorou MK, France J (Eds). Feeding systems and feed evaluation models. , Oxfordshire, UK: CAB International 2000; 35-52.
- Wilkins RJ. Potential digestibility of cellulose in grasses and its relationship with chemical and anatomical parameters. J Agric Sci 1972; 78: 457-464.
- Huhtanen P, Nousiainen J, Rinne M. Recent developments in forage evaluation with special reference to practical applications. Agr Food Sci 2006; 15: 293-323.
- Van Soest PJ. Nutritional ecology of the ruminant. 2nd ed. Ithaca, USA: Comstock Pub. Associates 1994; 476.
- Ellis WC, Poppi DP, Matis JH, et al. Dietary-digestive-metabolic interactions determining the nutritive potential of ruminant diets. Champaign, USA: American Society of Animal Science 1999; 423-481.
- Lippke H. Regulation of voluntary intake of ryegrass and sorghum forages in cattle by indigestible neutral detergent fiber. J Anim Sci 1986; 63: 1459-1468.
- Minson DJ. Forage in ruminant nutrition. San Diego, USA: Academic Press 1990.(483):9-59.
- Grant R. Forage fragility, fiber digestibility and chewing response in dairy cattle. In proceedings: Tri-state dairy nutrition conference. Columbus, USA 2010; 27-40.
- National Research Council. Nutrient requirements of dairy cattle. 7th ed. Washington DC, USA: National Academies Press 2001: 20-35.
- Krizsan SJ, Huhtanen P. Effect of diet composition and incubation time on feed indigestible neutral detergent fiber concentration in dairy cows. J Dairy Sci 2013; 96: 1715-1726.
- Feldsine P. AOAC International methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis. J AOAC Int 2002; 85: 1187-1200.
- Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74: 3583-3597.
- Mertens DR, Allen M, Carmany J, et al. Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles. Collaborative study. J AOAC Int 2002; 85: 1217-1240.
- Gomes DI, Detmann E, Filho SCV, et al. Evaluation of lignin contents in tropical forages using different analytical methods and their correlations with degradation of insoluble fiber. Anim Feed Sci Technol 2011; 168: 206-222.
- Cotanch KW, Grant RJ, Darrah J, et al. Development of a method for measuring forage fragility. J Dairy Sci 2007; 90 (Suppl. 1): 563.
- Lopes F, Ruh K, Combs DK. Validation of an approach to predict total-tract fiber digestibility using a standardized in vitro technique for different diets fed to high-producing dairy cows. J Dairy Sci 2015; 98:
2596-2602. - Chandler JA. Predicting methane fermentation bio-degradability. USA: Cornell University 1980; 93-107.
- Raffrenato E, Van Amburgh ME. Development of a mathematical model to predict sizes and rates of digestion of a fast and slow degrading pool and the indigestible NDF fraction. In proceedings: Cornell nutrition conference for feed manufacturers. New York, USA: 2010; 52-65.
- Mertens DR. Using uNDF to predict dairy cow performance and design rations. Belleville, USA: Mertens Innovation & Research LLC 2016; 12-19.
- Lucas HL. Stochastic elements in biological models, their sources and significance. In: Gurland J (Ed). Stochastic models in medicine and biology. Madison, USA: Univ Wisconsin Press 1964; 355-384.
- Mertens DR. Kinetics of cell wall digestion and passage in ruminants. In: Jung HG, Buxton DR, Hatfield RD, et al (Eds). Forage cell wall structure and digestibility. Madison, USA: American Society of Agronomy 1993; 535-570.
- Mertens DR, Taysom D, Steinlicht B. Factors affecting in vitro undigested NDF as estimates of indigestible NDF. J Dairy Sci 2012; 93: 291.
- Goering HK, Van Soest PJ. Forage fiber analysis (apparantus, reagents, procedures and some applications). Washington DC, USA: Agricultural Research Service 1970; 20.
- Rinne M, Huhtanen P, Jaakkola S. Grass maturity effects on cattle fed silage-based diets. Cell wall digestibility, digestion and passage kinetics. Anim Feed Sci Technol 1997; 67: 19-35.
- Rinne M, Huhtanen P, Jaakkola S. Digestive processes of dairy cows fed silages harvested at four stages of maturity. J Anim Sci 2002; 80: 1986-1998.
- Koukolová V, Wisbjerg MR, Hvelplund T, et al. Prediction of NDF degradation characteristics of grass and grass/clover forages based on laboratory methods. J Anim Feed Sci 2004; 13: 691-708.
- Mertens DR. Rate and extent of digestion. In: Dijkstra J, Forbes JM, France J (Eds). Quantitative aspects of ruminant digestion and metabolism. 2nd ed. Wallingford, UK: CAB International 2005; 13-47.
- Robinson PJ, Fadel JG, Tamminga S. Evaluation of mathematical models to describe neutral detergent residue in terms of its susceptibility to degradation in the rumen. Anim Feed Sci Technol 1986; 15:
249-271. - Van Soest PJ, Van Amburgh ME, Robertson JB, et al. Validation of the 2.4 times lignin factor for ultimate extent of NDF digestion, and curve peeling rate of fermentation curves into pools. In proceedings: Cornell nutrition conference for feed manufacturers. New York, USA 2005; 139-149.
- Huhtanen P, Asikainen U, Arkkila M, et al. Cell wall digestion and passage kinetics estimated by marker and in situ methods or by rumen evacuations in cattle fed hay 2 or 18 times daily. Anim Feed Sci Technol 2007; 133: 206-227.
- Raffrenato E, Fievisohn R, Cotanch KW, et al. Effect of lignin linkages with other plant cell wall components on in vitro and in vivo NDF digestibility of forages and potential energy yield. In proceedings: 3rd EAAP international symposium on energy and protein metabolism and nutrition. Parma, Italy 2010; 723-724.
- Krämer M, Weisbjerg MR, Lund P. Estimation of indigestible NDF in feedstuffs for ruminants. In proceedings: 1st Nordic feed science conference. Uppsala, Sweden 2010; 15-20.
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