Cytogenetic identification of Khuzestani water Buffalo

Document Type: Original Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine , Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Department of Genetics, Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

The domestic water buffalo (Bubalus bubalis) is an economically important livestock species in many Asian and Mediterranean countries. Two types of water buffalos are river and swamp buffaloes. Genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = 50). Khuzestan province is one of the important centers of buffalo farming in Iran. Since there was no cytogenetic information about Khuzestani buffalo breed, the present study was performed to provide karyotype and cytogenetic study in Khuzestan buffaloes. In this study, blood samples of 40 buffaloes were collected randomly from jugular vein via heparinized syringes. For metaphase smear, blood samples were cultured in RPMI 1640 medium containing fetal bovine serum and phitohemaglutination and then transferred to water bath for 69 hr. After stopping cell division by colcemide and using fixed solution, from each sample six slides were stained with Giemsa staining. Based on the results, number of chromosomes of the Khuzestani buffaloes was 50 (2n = 50). Among the 25 pairs of chromosomes, the first 5 pairs of autosomal chromosomes were sub-metacentric, 19 pairs of autosomal ones were acrocentric and the sex chromosomes (X and Y) were acrocentric. Furthermore, X and Y chromosomes were the largest and smallest acrocentric ones, respectively. Therefore, the buffaloes of Khuzestan province are considered as a river type.

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  1. Perera B. Reproductive cycles of buffalo. Anim Reprod Sci 2011; 124(3-4): 194-199.
  2. Perišić P, Bogdanović V, Mekić C, et al. The importance of buffalo in milk production and buffalo population in Serbia. Biotechnol Anim Husband 2015; 31(2): 255-263.
  3. Gordon I. Controlled reproduction in cattle and buffaloes. Acribia, SA Spain: CABI, 1999; 433-436.
  4. Di Berardino D, Iannuzzi L. Detailed description of RBA-banded chromosomes of river buffalo (Bubalus bubalis L.). Genet Sel Evol 1984; 16(3): 249-260.
  5. Ahmad I, Javed K, Sattar A. Screening of breeding bulls of different breeds through karyotyping. Pak Vet J 2004; 24(4): 190-192.
  6. Pournourali M, Tarang A, Mashayekhi F. Chromosomal analysis of two buffalo breeds of Mazani and Azeri from Iran. Iran J Vet Sci Technol 2015; 7(1): 22-31.
  7. Ali A, Abdullah M, Javed K, et al. Cytogenetic and genome studies in Pakistani buffalo (Bubalus bubalis)–A review. J Anim Plant Sci 2012; 22(3): 225-227.
  8. Murali N, Devendran P, Panneerselvam S. Cytogenetic studies on the chromosomes of Toda buffaloes. Buffalo Bull 2009; 28(2): 95-100.
  9. Pires R, Reichert R, Kasahara S. Cytogenetics of three breeds of river buffalo (Bubalus bubalis L.), with evidence of a fragile site on the X chromosome. Theriogenology 1998; 49(3): 529-538.
  10. Kenthao A, Tanomtong A, Supanuam P, et al. Standardized karyotype and idiogram of Mehsani buffaloes, Bubalus bubalis by conventional staining, GTG-banding, CBG-banding and Ag-NOR banding techniques. Buffalo Bull 2012; 31(1): 24-39.
  11. De Hondt H, Ghanam S. Cytogenetic studies of the Egyptian water buffalo (Bubalus bubalis). Z Tierzuecht Zuechtungsbiol 1971; 88(1-4): 64-68.
  12. Naserian AA, Saremi B. Water buffalo industry in Iran. Ital J Anim Sci 2007; 6(2): 1404-1405.
  13. Madad M, Hossein-Zadeh NG, Shadparvar AA. Genetic and phenotypic parameters for productive traits in the first three lactations of Khuzestan buffaloes in Iran. Archiv Tierzucht 2013; 56(41): 423-429.
  14. Borghese A. Development and perspective of buffalo and buffalo market in Europe and Near East. In proceedings: 9th World buffalo congress. Buenos Aires, Argentina. 2010: 20-31.
  15. Popescu P, Hayes H, Dutrillaux B. Techniques in animal cytogenetics. Berlin, Germany: Springer 2000; 65-68.
  16. Iannuzzi L. The water buffalo: evolutionary, clinical and molecular cytogenetics. Ital J Anim Sci 2007; 6(2): 227-236.
  17. Cribiu EP, Obeidah A, Boscher J. The C-banding pattern of the Egyptian Water Buffalo (Bubalus bubalis). Annal Genet Sel Anim 1978 10(2); 271-274.
  18. Iannuzzi L. Standard karyotype of the river buffalo (Bubalus bubalis L., 2n = 50). Report of the committee for the standardization of banded karyotypes of the river buffalo. Cytogenet Cell Genet 1994; 67 (2): 102-113.
  19. Yadav B, Kumar S, Tomer O, et al. Mosaicism of 50, XX/51, XX in a Murrah buffalo Bubalus bubalis. Genet Select Evol 1991; 23(5): 443-448.
  20. Sali KJ, Majeed MH. Cytogenetic study of river and swamp buffalo (Bubalus bubalus) in Iraq. Int J Curr Res 2012; 4: 144-146.
  21. Yavasoglu NUK, Koksal C, Yaman Y, et al. Karyotype of Anatolian water buffalo (Bubalus bubalis). Buffalo Bull 2014; 33(4): 437-442.
  22. Supanuam P, Tanomtong A, Jantarat S, et al. Standardized karyotype and idiogram of Thai native swamp buffalo, Bubalus bubalis (Artiodactyla, Bovidae) by convention staining, G-banding, C-banding and NOR-banding techniques. Thai J Genet 2010; 3(1): 83-93.
  23. Ali S, Ahmad Z, Mohiuddin G, et al. Studies on the karyotype of the Nili-Ravi buffalo. Pak Vet J 2001;21(2):72-76.
  24. Chandra H. Chromosomes of Asian water buffaloes, Bubalus bubalis (Linnaeus). Mamm Chrom Newsl 1968; 9: 231-236.
  25. Bongso T, Hilmi M. Chromosome banding homologies of a tandem fusion in river, swamp, and crossbred buffaloes (Bubalus bubalis). Can J Genet Cytol 1982; 24(6): 667-673.