Veterinary Research Forum

Vet Res Forum

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Diagnostic evaluation of milk lactate dehydrogenase and alkaline phosphatase activities by receiver operating characteristic analysis curve in early lactation of ewes with subclinical mastitis

Document Type : Original Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

Abstract

The aim of the present study was to investigate the diagnostic evaluation of milk lactate dehydrogenase (mLDH) and alkaline phosphatase (mALP) activities by receiver operating characteristic (ROC) analysis curve in early lactation of ewes with subclinical mastitis (SCM) and determine the correlation between number of somatic cell count (SCC) and mLDH and mALP activities. A total of 196 udder half milk samples were collected within the first 6 weeks of lambing. The SCM was determined by positive milk bacterial culture and positive California mastitis test (CMT); SCC was determined by fossomatic method and enzyme activities were determined spectrophotometrically. The mLDH and mALP of SCM cases were positively correlated with SCC values. Values of mLDH, mALP and SCC were significantly higher in SCM than non-SCM udder halves. The optimum cut-off points of mLDH and mALP activities for SCM diagnosis were determined at 203.61 (U L-1) and 329.84 (U L-1), respectively. In conclusion, SCC has positive correlation with mALP and mLDH activities in SCM ewes and mLDH and mALP activities could be considered as reliable indicators for intra-mammary inflammation diagnosis.

Keywords

Main Subjects

References

  1.  

    1. Stuhr T, Aulrich K. Intramammary infections in dairy goats: Recent knowledge and indicators for detection of subclinical mastitis. Landbauforschung 2010; 60(4): 267-279.
    2. Contreras A, Sierra D, Sánchez A, et al. Mastitis in small ruminants. Small Rum Res 2007; 68(1-2): 145-153.
    3. Sani RN, Mahdavi A, Moezifar M. Prevalence and etiology of subclinical mastitis in dairy ewes in two seasons in Semnan province, Iran. Trop Anim Health Pro 2015; 47(7): 1249-1254.
    4. Schalm OW, Carroll EJ, Jain NC. Bovine mastitis. In: Jain NC (Ed). Essentials of veterinary hematology. Philadelphia, USA: Lea & Febiger 1971; 94-183.
    5. Sears P, Gonzalez R, Wilson DJ, et al. Procedures for mastitis diagnosis and control. Vet Clin North Am Food Anim Pract 1993; 9(3): 445-468
    6. Witt D, Trendelenburg C. Joint study to establish benchmarks for clinical-chemical parameters in child-hood [German]. J Clin Chem Clin Biochen 1982; 20(4): 235. doi: https://doi.org/10.1515/cclm.1982.20.4.235.
    7. Schlebusch H, Rick W, Lang H, et al. Standards in the activities of clinically important enzymes. Dtsch Med Wochenschr 1974; 99 (15): 765-766.
    8. Batavani R, Mortaz E, Falahian K, et al. Study on frequency, etiology and some enzymatic activities of subclinical ovine mastitis in Urmia, Iran. Small Rum Res 2003; 50(1-2): 45-50.
    9. Las Heras A, Dominguez L, Fernandez-Garayzabal JF. Prevalence and aetiology of subclinical mastitis in dairy ewes of the Madrid region. Small Rum Res 1999; 32: 21-29.
    10. Brade W. Udder health, somatic cell content and milk quality [German]. Tierärztl Umsch 2001; 56: 470-476.
    11. Raynal-Ljutovac K, Pirisi A, De Cremoux R, et al. Somatic cells of goat and sheep milk : analytical, sanitary, productive and technological aspects. Small Rumin Res 2007; 68(1-2): 26-144.
    12. Corrales JC, Sanchez A, Luengo C, et al. Effect of clinical contagious agalactia on the bulk tank milk somatic cell count in Murciano-Granadina goat herds. J Dairy Sci 2004; 87 (10): 3165-3171.
    13. Katsoulos PD, Christodoulopoulos G, Minas A, et al. The role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats. J dairy Res 2010; 77(1): 107-111.
    14. Babaei H, Mansouri-Najand L, Molaei M, et al. Assessment of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase activities in cow’s milk as an indicator of subclinical mastitis. Vet Res Commun 2007; 31(4): 419-425.
    15. Paape M, Wiggans G, Bannerman DD, et al. Monitoring goat and sheep milk somatic cell counts. Small Rum Res 2007; 68: 114-125.
    16. Oliszewski R, Nunez de Kairuz M, Gonzalez De Elias S, et al. Assessment of β-glucuronidase levels in goat's milk as an indicator of mastitis: Comparison with other mastitis detection methods. J Food Protect 2002; 65: 864-866.
    17. Guha A, Gera S, Sharma A. Evaluation of milk trace elements, lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase activity of subclinical mastitis as and indicator of subclinical mastitis in riverine buffalo (Bubalus bubalis). Asian-Aust J Anim Sci 2012; 25(3): 353-363.
    18. Gonzalo C, Carriedo J, Gomez J, et al. Diurnal variation in the somatic cell count of ewe milk. J dairy Sci 1994; 77(7): 1856-1859.
    19. Gonzalo C, Ariznabarreta A, Carriedo J, et al. Mammary pathogens and their relationship to somatic cell count and milk yield losses in dairy ewes. J dairy Sci 2002; 85(6): 1460-1467.
    20. Gonzalo C, Carriedo J, Blanco M, et al. Factors of variation influencing bulk tank somatic cell count in dairy sheep. J dairy Sci 2005; 88(3): 969-974.
    21. McDougall S, Murdough P, Pankey W, et al. Relationships among somatic cell count, California mastitis test, impedance and bacteriological status of milk in goats and sheep in early lactation. Small Rum Res 2001; 40(3): 245-254.
    22. Kalogridou-Vassiliadou D, Manolkidis K, Tsigoida A. Somatic cell counts in relation to infection status of the goat udder. J Dairy Res 1992; 59(1): 21-28.
    23. Beheshti R, Shaieghi J, Eshratkhah B, et al. Prevalence and etiology of subclinical mastitis in ewes of the Tabriz region, Iran. Glob Vet 2010; 4(3): 299-302.
    24. Hueston WD, Hartwig NR, Judy JK. Detection of intramammary infection with the California mastitis test. J Am Vet Med Assoc 1986; 188: 522-524.
    25. De Cremoux R, Poutrel P. Cell counts in the goat: A presumptive diagnostic tool for breast infections [French]. In Proceedings: 7th international conference on goats. Poitiers, France: 2000; 757-760.
    26. Symons D, Wright, LJ. Changes in bovine mammary gland permeability after intramammary exotoxin infusion. J Comp Path 1974; 84(1): 9-17.
    27. Batavani RA, Asri S, Naebzadeh H. The effect of subclinical mastitis on milk composition in dairy cows. Iran J Vet Res 2007; 8(3): 205-211.
    28. Mohammadian B. The Effect of subclinical mastitis on lactate dehydrogenase in dairy cows. Int J Anim Vet Adv 2011; 3(3): 161-163.
    29. Rasouli A, Mosaferi S. Accuracy rate of mastitis on-farm test (udder check) in comparison to somatic cell count (S.C.C) in dairy herd. J Livestock Sci 2017; 8: 191-195.
    30. Zeinhom MMA, Abed AH, Hashem KS. A contribution towards milk enzymes, somatic cell count and bacterial pathogens associated with subclinical mastitis cows milk. Assiut Vet Med J 2013; 59(138): 38-48.
    31. Singh M, Sharma A, Mittal D, et al. Assessment of lactate dehydrogenase enzyme activity in milk as a marker for detection of subclinical mastitis. J Anim Res 2016; 6(2): 113-116.
Veterinary Research Forum
Volume 9, Issue 4
December 2018
Pages 343-348
Files
History
  • Receive Date: 12 November 2018
  • Accept Date: 12 November 2018
Share
How to cite
Statistics