Evaluation of oxidant/antioxidant status in serum of sheep experimentally envenomated with Hemiscorpius lepturus scorpion venom

Document Type: Short Communication

Authors

1 PhD Candidate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Animal Health Management, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

6 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, shahrekord, Iran

Abstract

Scorpion envenomation is a main general health problem in developing countries, especially in tropical and subtropical regions. Hemiscorpius lepturus as a member of the Hemiscorpiidae family is cause of the most scorpion sting lethality in Iran. In the present study, the oxidative stress and antioxidant defense in serum of envenomated sheep with the venom of Hemiscorpius lepturus were investigated. Nine sheep were randomly divided into three groups (three in each). Groups A, B and C received 0.10, 0.05 and 0.01 mg kg-1 of H. lepturus venom subcutaneously, respectively. Blood sampling were performed 30 min before envenomation (control) and 30 min, 1, 2, 3 and 6 hr after envenomation and serum levels of total antioxidant capacity (TAC), malonedialdehyde (MDA) and protein carbonyl (PCO) were determined. The TAC was significantly increased at the doses of 0.10 mg kg-1 (at 3 hr) and 0.05 mg kg-1 (at 6 hr) compared to pre-injection time. However, no significant differences were observed in serum levels of MDA and PCO in different groups. It can be concluded that the dose of 0.01 mg kg-1 of venom had no effect on stress factors of serum, but according to increased level of TAC at the doses of 0.05 and 0.10 and no significant changes in serum levels of MDA and PCO, the oxidative damage has been prevented by the antioxidant defense system response.

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  1. Salman MMA. Oxidative stress and biochemical adaptations of scorpion (Leiurus quinquestriatus) crude venom in albino rats. Egypt Acad J Biolog Sci 2015; 7(1): 121-133.
  2. Radmanesh M. Cutaneous manifestations of the Hemiscorpius lepturus sting: A clinical study. Int J Dermatol 1998; 37(7): 500-507.
  3. Navidpour S, Kvarik F, Soleglad ME, et al. Scorpions of Iran (Arachnida, Scorpiones). Part I. Khoozestan province. Euscorpius 2008; 65: 1-41.
  4. Satoh H, Nishida S. Electropharmacological actions of Ginkgo biloba extract on vascular smooth and heart muscles. Clin Chim Acta 2004; 342(1): 13-22.
  5. de Roodt AR, Garcia SI, Salomon OD, et al. Epidemiological and clinical aspects of scorpionism by Tityus trivittatus in Argentina. Toxicon 2003; 41(8): 971-977.
  6. Freire-Maia L, Campos A. On the treatment of cardiovascular manifestations of scorpion envenomation. Toxicon 1987; 25(2): 125-130.
  7. Khorchid A, Fragoso G, Shore G, et al. Catecholamine induced oligodendrocyte cell death in culture is developmentally regulated and involves free radical generation and differential activation of caspase. Glia 2002; 40(3): 283-299.
  8. Rahravani M, Ghadrdan-Mashhadi A, Rasooli A, et al. Histopathological study of Hemiscorpius lepturus venom injection in sheep. Irani Vet J 2015; 11(3): 46-55.
  9. Javaheri Koupaei M, Ghadrdan-Mashhadi A, Rasooli A, et al. Effects of different time protocols treatment on coagulation parameters of sheep injected with Hemiscorpius lepturus venom. Jundishapur J Nat Pharm Prod 2016; 11(2): e30065. doi: 10.17795/jjnpp-30065.
  10. Farzanpei R. Identification of scorpions [Persian]. 1st ed. Tehran, Iran: Tehran University Press 1989; 191.
  11. Plaser ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malondialdehyde) in biochemical systems. Anal Biochem 1966; 16(2): 359-364.
  12. Satoh K. Serum lipid peroxidation in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978; 90(1): 37-43.
  13. Reznick AZ, Packer L. Oxidative damage to proteins: Spectrophotometric method for carbonyl. Methods Enzymol 1994; 233: 357-363.
  14. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: The FRAP assay. Anal Biochem 1996; 239(1): 70-76.
  15. Yu BP. Cellular defenses against damage from reactive oxygen species. Physiol Rev 1994; 74(1): 139-162.
  16. Laraba-Djebari F, Kabrine M. Phytotherapy as new approach to treat scorpion envenomation: Experimental study. Int J Pharm Sci Res 2014; 5 (5): 1682-1692.
  17. Dousset E, Carrega L, Steinberg JG, et al. Evidence that free radical generation occurs during scorpion envenomation. Comp Biochem Physiol C Toxicol Pharmacol 2005; 140(2): 221-226.
  18. Dalle-Donne I, Rossi R, Giustarini D, et al. Protein carbonyl groups as biomarkers of oxidative stress. Clin Chimi Acta 2003; 329(1-2): 23-38.