Crocin exerts improving effects on indomethacin-induced small intestinal ulcer by antioxidant, anti-inflammatory and anti-apoptotic mechanisms

Ghafarzadeh, Sadat; Hobbenaghi, Rahim; Tamaddonfard, Esmaeal; Farshid, Amir Abbas; Imani, Mehdi

doi:10.30466/vrf.2018.93512.2256

Document Type : Original Article

Authors

¹ Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University

² Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

³ Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2018.93512.2256

Abstract

Crocin is a plant-derived carotenoid and bears potent antioxidant property. Ranitidine (a histamine H₂ receptor blocker) is used for peptic ulcer treatment. The present study was planned to investigate the effects of crocin and ranitidine on indomethacin-induced ulcer in small intestine of rats. Animals were randomized into two major groups including indo-methacin (10.00 mg kg^-1, ulcer group, 48 rats) and normal saline (1.00 mL kg^-1, intact group, 48 rats) groups. Each of these two major groups was subdivided into eight subgroups for intra-peritoneal (IP) injections of normal saline, crocin (2.50, 10.00 and 40.00 mg kg^-1), ranitidine (5.00 and 20.00 mg kg^-1), crocin (2.50 and 10.00 mg kg^-1) plus ranitidine (5.00 mg kg^-1). Indomethacin induced intestinal ulcer was characterized by bleeding, inflammation, epithelial hyperplasia and crypt loss. This non-steroidal anti-inflammatory drug (NSAID), indomethacin decreased goblet cell number and superoxide dismutase (SOD) activity and increased small intestine weight, organo-somatic index (OSI), malodealdehyde (MDA), tumor necrosis factor-α (TNF-α) and caspase-3 contents of intestine. Crocin resolved all the above-mentioned parameter changes induced by indomethacin. These treatments produced no significant effects on the above-mentioned parameters of intact group. The results of the present study showed tissue protective and anti-ulcer effects of crocin on small intestine by antioxidant, anti-inflammatory and anti-apoptotic mechanisms. Ranitidine alone showed no effect; however, in combination with crocin it exerted recovery effects. It is recommended that crocin, be considered as a therapeutic agent for NSAIDs-induced intestinal damage management.

Keywords

Main Subjects

Pathology

References

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Veterinary Research Forum

Crocin exerts improving effects on indomethacin-induced small intestinal ulcer by antioxidant, anti-inflammatory and anti-apoptotic mechanisms

References

References

Volume 10, Issue 4
December 2019
Pages 277-284

Crocin exerts improving effects on indomethacin-induced small intestinal ulcer by antioxidant, anti-inflammatory and anti-apoptotic mechanisms

References

References

Volume 10, Issue 4December 2019Pages 277-284

Volume 10, Issue 4
December 2019
Pages 277-284