Attenuating effects of Mangifera indica leaves ethanolic extract against acetamiprid induced reproductive toxicity in male guinea pigs

Nounamo Guiekep, Arthénice Jemima; Kenfack, Augustave; Ngoula, Ferdinand; Vemo, Bertin Narcisse; Nguemmeugne, Kenmeuhe Sidje; Pamo Tedonkeng, Etienne

doi:10.30466/vrf.2019.95154.2292

Document Type : Original Article

Authors

Department of Animal Productions, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang, Cameroon

https://doi.org/10.30466/vrf.2019.95154.2292

Abstract

Acetamiprid (ACP) belonging to the neonicotinoid family used against wide array of pests in agriculture and domestic purposes. In this study, we evaluated the attenuating effects of ethanolic extract of Mangifera indica leaves (EEMI) in averting reproductive toxicity caused by ACP in male guinea pigs. Thirty male guinea pigs were randomly assigned to five treatment groups (n = 6). Group 1 (T0) received distilled water orally; group 2 (T0-) was given 80 mg kg^-1of ACP and groups 3, 4 and 5 were treated, respectively, with EEMI at doses of 50, 100 and 200 mg kg^-1 plus ACP. After 90 days, the reaction time, sexual organ weights, sperm count, motility and anomalies, spermatozoa with entire plasma membrane, testicular histology, serum testosterone concentration, testicular malondialdehyde (MDA) level, reduced glutathione (GSH) concentration, testicular superoxide dismutase (SOD) and catalase (CAT) activities were assessed. Co-administration of EEMI significantly reduced the reaction time, sperm anomalies and testicular MDA, SOD and CAT levels compared to the T0- group. Co-treatment of EEMI significantly alleviated sperm count and motility, percentage of spermatozoa with the normal plasma membrane, serum testosterone concentration, accessory sex gland weights, and testicular GSH concentrations. The ACP treatment induced cell membrane degradation in the testis and this effect was prevented with the addition of EEMI. In conclusion, ACP negatively affected the animal reproductive function and induced oxidative stress. The addition of EEMI alleviated the toxic effects of ACP on the reproductive function of male guinea pigs.

Keywords

Main Subjects

Physiology

References

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Veterinary Research Forum

Attenuating effects of Mangifera indica leaves ethanolic extract against acetamiprid induced reproductive toxicity in male guinea pigs

References

References

Volume 10, Issue 3
September 2019
Pages 187-192

Attenuating effects of Mangifera indica leaves ethanolic extract against acetamiprid induced reproductive toxicity in male guinea pigs

References

References

Volume 10, Issue 3September 2019Pages 187-192

Volume 10, Issue 3
September 2019
Pages 187-192