Veterinary Research Forum
Vet Res Forum

A bioassay on tissue cholinesterase activity of Rutilus kutum (Kamensky, ‎‎1901) exposed to some common pesticides in Iran

Document Type : Original Article

Authors

Nima Shiry‎ 1
Seyed Jalil Alavinia 2, 3
Amin Gholamhosseini 4
Alireza Mirvaghefi 3

1 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran ‎

2 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran

4 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

https://doi.org/10.30466/vrf.2018.90805.2201
Abstract
The toxicological effects of three commonly used pesticides in Iran on the fries of Caspian kutum (Rutilus kutum, Kamensky, 1901) were investigated through determining 50% lethal concentration (LC50) 96‑hr and cholinesterase (ChE) inhibition. The LC50 96-hr of carbaryl, glyphosate, and malathion were equal to 11.69, 6.64, and 0.97 mg L-1, respectively, that were considered as harmful (10.00-100 mg L-1), toxic (1.00-10.00 mg L-1) and very toxic (-1) compounds for this species. The exposure of fries to sub-lethal concentrations of the pesticides over 15 days indicated that the average of ChE activity in the head and trunk were 1086.89 ± 124.34 and 627.36 ± 99.60 mU min-1 per mg protein, respectively, with a significant difference relative to each other. There was a significant difference between fry exposed to all three pesticides and the control group in cholinesterase inhibition. The fries exposed to carbaryl (890.12 ± 28.08 mU min-1 per mg protein) and glyphosate (891.77 ± 31.61 mU min-1 per mg protein) showed lower ChE inhibition than those exposed to malathion (790.00 ± 58.14 mU min-1 per mg protein).

Keywords

Carbaryl
‎ ChE activity
‎ Glyphosate
‎ Malathion
‎ Rutilus kutum
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Veterinary Research Forum
Volume 11, Issue 4
Autumn 2020
Pages 325-331

  • Receive Date 27 July 2018
  • Revise Date 09 December 2018
  • Accept Date 29 December 2018

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