A bioassay on tissue cholinesterase activity of Rutilus kutum (Kamensky, ‎‎1901) exposed to some common pesticides in Iran

Document Type: Original Article

Authors

1 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran ‎

2 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran

4 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

10.30466/vrf.2018.90805.2201

Abstract

The toxicological effects of three commonly used pesticides in Iran on the fries of Caspian kutum (Rutilus kutum, Kamensky, 1901) were investigated through determining 50% lethal concentration (LC50) 96‑hr and cholinesterase (ChE) inhibition. The LC50 96-hr of carbaryl, glyphosate, and malathion were equal to 11.69, 6.64, and 0.97 mg L-1, respectively, that were considered as harmful (10.00-100 mg L-1), toxic (1.00-10.00 mg L-1) and very toxic (<1.00 mg L-1) compounds for this species. The exposure of fries to sub-lethal concentrations of the pesticides over 15 days indicated that the average of ChE activity in the head and trunk were 1086.89 ± 124.34 and 627.36 ± 99.60 mU min-1 per mg protein, respectively, with a significant difference relative to each other. There was a significant difference between fry exposed to all three pesticides and the control group in cholinesterase inhibition. The fries exposed to carbaryl (890.12 ± 28.08 mU min-1 per mg protein) and glyphosate (891.77 ± 31.61 mU min-1 per mg protein) showed lower ChE inhibition than those exposed to malathion (790.00 ± 58.14 mU min-1 per mg protein).

Keywords



Articles in Press, Corrected Proof
Available Online from 29 September 2020
  • Receive Date: 27 July 2018
  • Revise Date: 09 December 2018
  • Accept Date: 29 December 2018