Molecular characterization and Phylogenetic analysis of foot and mouth disease virus isolates in Sulaimani province, Iraq

Document Type: Short Communication

Authors

1 Department of Molecular biology- veterinary laboratory center, Sulaimani veterinary directorate

2 Department of Molecular biology- veterinary laboratory center, Sulaimani veterinary directorate.

10.30466/vrf.2019.101755.2424

Abstract

Foot-and-mouth disease (FMD) is endemic in Iraq, The current study can be considered as the first molecular characterization of serotype O in Iraq, The present investigation reports the determine of serotype FMDV type O from local farms in Sulaimani districts in 2016 outbreaks, samples were collected from suspected in cattle. The virus was primarily detected with RT-PCR 1F/1R universal primer pairs directly from mouth epithelial field sample. The direct sequencing and subsequent analysis of amplified PCR products for the VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, Phylogenetic analysis revealed that the field isolated serotype O belonged to Topotype ME-SA and lineage panasiaII and clustered with (Pakistan and Iran) isolate (KU365843 and KY091283) respectively with identity (96%, 95%) among them. Furthermore, in a phylogenic tree, it had different lineage with the three O1 Manisa and Iraq/2000 strain. these findings highlight the continuous circulation of serotype O of FMD in the region

Keywords



Articles in Press, Accepted Manuscript
Available Online from 29 September 2020
  • Receive Date: 14 January 2019
  • Revise Date: 17 August 2019
  • Accept Date: 30 October 2019