Poultry
Hadi Shabani; Forough Talazade; Gholam Abbas Kaydani; Masoud Reza Seyfi
Volume 15, Issue 3 , March 2024, , Pages 151-158
Abstract
An internationally recognized syndrome that leads to deaths among domestic and ornamental pigeons, particularly after racing, is young pigeon disease syndrome (YPDS). Pigeon circovirus (PiCV) is regarded as one of the potential factors contributing to the occurrence of YPDS. This survey was conducted ...
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An internationally recognized syndrome that leads to deaths among domestic and ornamental pigeons, particularly after racing, is young pigeon disease syndrome (YPDS). Pigeon circovirus (PiCV) is regarded as one of the potential factors contributing to the occurrence of YPDS. This survey was conducted to determine the prevalence of PiCV infection and molecularly characterize the PiCV in pigeons suspected of YPDS. Eighty fecal samples were collected from 80 diseased pigeons (exhibiting symptoms such as lethargy, weight loss, crop stasis, vomiting and diarrhea) from 20 lofts in different areas of Ahvaz, Iran. Also, 20 fecal samples were obtained from 20 clinically healthy pigeons. The nested broad spectrum polymerase chain reaction test was done to identify the circovirus, using primers targeting part of the replication-associated protein gene with 350 bp, and several positive samples were sequenced. This study showed that PiCV was detected in 86 out of the 100 samples (86.00%). Two types of circoviruses were determined in the samples. One type of the detected circoviruses was PiCV which based on phylogenetic analysis had high genetic similarity with A, B, G and H genotypes of PiCV. The other type of detected circoviruses was closely related to beak and feather disease virus (BFDV) which causes one of the most significant viral diseases in psittacine birds. This is the first report of BFDV identification in pigeons.
Small Animal Internal Medicine
Forough Talazadeh; Masoud Ghorbanpoor; Milad Masoudinezhad
Volume 14, Issue 8 , August 2023, , Pages 431-436
Abstract
The current study was conducted to survey the prevalence of pigeon candidiasis in diseased pigeons suspected to candidiasis by isolation, microscopic examination, and polymerase chain reaction (PCR) method and to characterize Candida spp. phylogenetically. For this purpose, samples were obtained from ...
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The current study was conducted to survey the prevalence of pigeon candidiasis in diseased pigeons suspected to candidiasis by isolation, microscopic examination, and polymerase chain reaction (PCR) method and to characterize Candida spp. phylogenetically. For this purpose, samples were obtained from 100 suspected pigeons from September 2018 to February 2019 in Ahvaz, Iran. Cloacal and oropharyngeal swab samples were collected from each diseased pigeon with diarrhea resistant to the antibiotics, crop stasis, white diphtheritic membrane in the mouth, regurgitation, and vomiting. Sabouraud dextrose agar was used as a culture medium. Selected colonies were stained with lactophenol cotton blue stain. In the culture and direct microscopic observation, 19.00% of birds were suspected to candidiasis. Twenty-two isolates were identified. All 22 isolates were confirmed as Candida spp. By PCR method. The PCR test confirmed the presence of Candida spp. in 19.00% of pigeons. Based on the sequencing results of some PCR products, the isolates belonged to Candida albicans and Candida glabrata. The results revealed a 99.78% accordance when compared with other sequences of C. albicans which were formerly deposited in GenBank® from Colombia, Indonesia, China, and Sudan. The results revealed a 99.54% accordance when compared with other sequences of C. glabrata which were formerly deposited in GenBank® from the Netherlands and Spain. The symptoms such as diarrhea resistant to antibiotics, crop stasis, white diphtheritic membrane in the mouth, regurgitation, and vomiting were the most prevalent clinical symptoms in positive pigeons.
Elham Rahimi Sardo; Forough Talazadeh; Ramezan Ali Jafari; Masoud Reza Seyfi
Volume 14, Issue 6 , June 2023, , Pages 329-334
Abstract
An internationally identified syndrome that leads to deaths between domestic and ornamental pigeons, particularly after racing is young pigeon disease syndrome (YPDS). This study was conducted to determine the status of pigeon adenoviral infection and molecularly characterize the pigeon adenovirus in ...
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An internationally identified syndrome that leads to deaths between domestic and ornamental pigeons, particularly after racing is young pigeon disease syndrome (YPDS). This study was conducted to determine the status of pigeon adenoviral infection and molecularly characterize the pigeon adenovirus in Ahvaz pigeons. Sixty stool samples of healthy pigeons (young pigeons and adult pigeons) and 60 stool samples of diseased pigeons (young and adults) with symptoms of lethargy, weight loss, crop stasis, vomiting and diarrhea were examined. Samples were screened for aviadenoviruses by polymerase chain reaction (PCR) assay and degenerated primers set to target the aviadenovirus polymerase (pol) gene were used which was designed in this study. Screening for pigeon adenovirus 1 (PiAdV-1) was performed using a primer pair that targeted the fiber gene of PiAdV-1. Out of 120 stool samples, six samples (5.00%) were positive for aviadenovirus. The results showed that independent from pigeons’ age status, 5.00 and 3.33% of sick and of healthy pigeons were positive for PiAdV-1, respectively. Genomic sequencing revealed that the viruses detected in Ahvaz pigeons belonged to the PiAdV-1 genotype. The results in pigeons revealed a 98.10 - 99.53% nucleotide similarity when compared to other strains of PiAdV-1 (TR/SKPA20, P18-05523-6 and strain IDA4) formerly deposited in GenBank® in Türkiye, Australia and The Netherlands. As far as the authors know, this was the first record of phylogenetic analysis of PiAdV-1 in Iran.
Forough Talazadeh; Masoud Ghorbanpoor; Yasaman Bahadori
Volume 14, Issue 5 , May 2023, , Pages 281-287
Abstract
Macrorhabdus ornithogaster is a microorganism that causes nonspecific and general clinical symptoms and to this day, diagnosis and also treatment have been yet hard. The present study was conducted to survey the prevalence of macrorhabdosis and to characterize M. ornithogaster phylogenetically in Psittaciformes ...
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Macrorhabdus ornithogaster is a microorganism that causes nonspecific and general clinical symptoms and to this day, diagnosis and also treatment have been yet hard. The present study was conducted to survey the prevalence of macrorhabdosis and to characterize M. ornithogaster phylogenetically in Psittaciformes suspected of macrorhabdosis from January 2018 to May 2019 in Ahvaz, Iran. For this purpose, fecal samples were collected from Psittaciformes with signs of the disease. Wet mounts were prepared from fecal samples and examined carefully using a light microscope. Samples from parrots with gastrointestinal symptoms of the disease were chosen for molecular diagnosis of the organism and DNA was extracted from these samples. For detection of M. ornithogaster, primer sets (BIG1, Sm4) and (AGY1, Sm4) which target the 18S rDNA gene were selected and Semi-nested polymerase chain reaction (Semi-nested PCR) was performed. The PCR method confirmed the presence of M. ornithogaster in 14.00% of the samples. Purified PCR products were sequenced for more accurate confirmation and according to the gene sequence all sequences were owned by M. ornithogaster. The results disclosed a 96.03% - 100% identity when compared to other sequences of M. ornithogaster which had previously been deposited in the GenBank® from Germany and the USA. The results of this study proved the circulation of M. ornithogaster between cockatiel, budgerigar and grey parrot. The prevalence of macrorhabdosis was higher in cockatiel compared to budgerigar and grey parrot. As far as the authors know, this was the first record of macrorhabdosis in African grey parrots.
