Veterinary Research Forum

Abstract This report presented a case of the concurrent diagnosis of early feline diffuse iris melanoma (FDIM) and metastatic cutaneous melanoma in a tricolored mixed stray cat. Histopathological examination of biopsy samples identified melanoma on the nasal planum, lips along with metastasis to the submandibular lymph nodes. The initial treatment included surgical resection of the primary nasal planum neoplasm and the metastatic lesions in the submandibular lymph nodes. The necropsy, performed 40 days after the surgery, revealed the metastatic nodules in multiple organs including the liver, lungs, thyroids, pericardium, pleura, peritoneum, kidneys, and numerous lymph nodes. Histopathological examination revealed a biphasic neoplastic tissue composed of a mixture of epithelioid and spindle cells in primary and metastatic tumors. Additionally, the anterior surface of the iris contained 4 - 5 rows of melanocytic interstitial infiltration indicative of early FDIM. Immunohistochemical analysis revealed that the neoplastic cells showed positive immunoreactivity for Melan-A. To the authors' knowledge, this case represented the first reported case of concurrent FDIM and cutaneous melanoma in a feline patient.

Abstract Moellerella wisconsensis is a Gram-negative, facultative anaerobic bacillus of Entero-bacteriaceae family, and it is an uncommon pathogen in domestic animals. To date, five cases were reported including two dogs, two cattle, and a goat. Streptococcus equisimilis is the second common bacterial agent after the S. equi subsp. zooepidemicus in equine pneumonia cases. The present report describes the isolation of M. wisconses from lungs and spleen of a 10-year-old Arabian horse (May 08, 2022) at post-mortem examination being co-infected with S. equisimilis. Clinical and pathological findings included bilateral nasal discharge, conjunctivitis, sternal recumbency, severe diffuse necrosuppurative rhinitis, multi-focal fibrinopurulent pneumonia and purulent lymphadenitis. Polymerase chain reaction assays showed no viral nucleic acids of equid alphaherpesvirus (EHV) 1, EHV-4, equine arteritis virus and equine papilloma virus. The antibiogram test revealed that the isolate was sensitive to several antibiotics except colistin. Taken together, the present report documents the first isolation of M. wisconsensis from lungs and spleen of a horse; hence, experimental studies are needed to clarify the pathogenity and pathogenesis of M. wisconsensis.