Genetic
Qiang Liu; Yue-Yang Yu; Huai-Yu Wang
Volume 14, Issue 10 , October 2023, , Pages 531-539
Abstract
The jaagsiekte sheep retrovirus (JSRV), belonging to the betaretrovirus genus of the retroviridae family, includes both exogenous and endogenous jaagsiekte sheep retroviruses (exJSRV and enJSRV, respectively). At the proviral genome level, exJSRV and enJSRV strains have a high degree of similarity with ...
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The jaagsiekte sheep retrovirus (JSRV), belonging to the betaretrovirus genus of the retroviridae family, includes both exogenous and endogenous jaagsiekte sheep retroviruses (exJSRV and enJSRV, respectively). At the proviral genome level, exJSRV and enJSRV strains have a high degree of similarity with their main variation regions being the LTR, gag, and env genes. In this study, for the first time, we investigated and compared the distribution of CpG islands between these enJSRV and exJSRV strains. Specifically, we analyzed a total of 42 full-length JSRV genomic sequences obtained from the GenBank® database to identify CpG islands in the exJSRV and enJSRV genomes using the MethPrimer software. Our results showed that the CpG islands in the two JSRV strains were mainly distributed in the LTR, gag, and env genes. In exJSRVs, 66.66% (6/9), 33.33% (3/9), and 100% (9/9) of the sequences presented at least one CpG island in LTR, gag, env genes, respectively, and for enJSRVs, 84.84% (28/33), 57.57% (19/33), and 96.96% (32/33) of the sequences presented at least one CpG island in the LTR, gag, and env genes. These findings suggested that the distribution, length, and genetic traits of CpG islands were different for the exJSRV and enJSRV strains. In future, it would be necessary to demonstrate the biological significance of CpG islands within these genes in exJSRV and enJSRV genomes. This will enhance understanding regarding the potential role of CpG islands in epigenetic regulation.
Genetic
Javad Alikhani; Godratollah Mohammadi; Gholamreza Shariati
Volume 9, Issue 4 , December 2018, , Pages 357-360
Abstract
The domestic water buffalo (Bubalus bubalis) is an economically important livestock species in many Asian and Mediterranean countries. Two types of water buffalos are river and swamp buffaloes. Genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = ...
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The domestic water buffalo (Bubalus bubalis) is an economically important livestock species in many Asian and Mediterranean countries. Two types of water buffalos are river and swamp buffaloes. Genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = 50). Khuzestan province is one of the important centers of buffalo farming in Iran. Since there was no cytogenetic information about Khuzestani buffalo breed, the present study was performed to provide karyotype and cytogenetic study in Khuzestan buffaloes. In this study, blood samples of 40 buffaloes were collected randomly from jugular vein via heparinized syringes. For metaphase smear, blood samples were cultured in RPMI 1640 medium containing fetal bovine serum and phitohemaglutination and then transferred to water bath for 69 hr. After stopping cell division by colcemide and using fixed solution, from each sample six slides were stained with Giemsa staining. Based on the results, number of chromosomes of the Khuzestani buffaloes was 50 (2n = 50). Among the 25 pairs of chromosomes, the first 5 pairs of autosomal chromosomes were sub-metacentric, 19 pairs of autosomal ones were acrocentric and the sex chromosomes (X and Y) were acrocentric. Furthermore, X and Y chromosomes were the largest and smallest acrocentric ones, respectively. Therefore, the buffaloes of Khuzestan province are considered as a river type.
Genetic
Mohammadreza Ghorani; Arash Ghalyanchi Langeroudi; Omid Madadgar; Mohammadreza Rezapanah; Sedigheh Nabian; Reza Khaltabadi Farahani; Hossein Maghsoudloo; Mohammad Forsi; Hamed Abdollahi; Hesameddin Akbarein
Volume 8, Issue 4 , December 2017, , Pages 287-292
Abstract
Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, ...
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Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular identification. Three positive samples were detected by RT-PCR. All positive samples were confirmed by sequencing. The phylogenetic tree which displays the molecular relationship between the viruses of different Iranian geographic regions and references isolates was constructed. The Iranian isolates formed two distinct phylogenetic groups (Group 1 and Group 2). The IR-CPV-GMG-1, IR-CPV-GMG-2, IR-CPV-GMG-4, and IR-CPV-GMG-6 formed Group 1 and IR-CPV-GMG-3, IR-CPV-GMG-5, and IR-CPV-GMG-7 were in Group 2 as a distinct group. Iranian isolates in group 1 were similar to European and East Asian CBPVs. This research was the first phylogenetic analysis of CBPV in Iran. Further researches are needed to study the other aspects of this virus-like genetic characteristics and pathogenesis in Iran.
Microbiology
Asma Afshari; Abdollah Jamshidi; Jamshid Razmyar; Mehrnaz Rad
Volume 6, Issue 4 , December 2015, , Pages 279-284
Abstract
Clostridium perfringens is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from ...
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Clostridium perfringens is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from broilers’ meat marketed in retail meat shops of Mashhad city in Northeastern of Iran. After isolation of C. perfringens using conventional culture method and confirmation by specific 16S rDNA gene, a multiplex polymerase chain reaction assay with specific primers, were performed for toxin typing of isolates. Clostridium perfringens was isolated from 31 broilers’ meat samples (15.50%) out of 200 samples and for toxin typing the results showed 9 isolates as type A (29.03%) and 22 isolates as type C (70.96%). In this study, cpe-positive C. perfringens were detected in eight isolates of type C (25.00%). Our results indicated that C. perfringens type C is the most common type in broiler chicken carcasses.
