@article { author = {Ghaderzadeh, Shirzad and Mirzaei Aghjehgheshlagh, Farzad and Nikbin, Saeid and Navidshad, Bahman}, title = {Stimulatory effects of nano-selenium and conjugated linoleic acid ‎ on antioxidant activity, trace minerals, and gene expression response of growing male Moghani lambs}, journal = {Veterinary Research Forum}, volume = {11}, number = {4}, pages = {385-391}, year = {2020}, publisher = {Faculty of Veterinary Medicine, Urmia University}, issn = {2008-8140}, eissn = {2322-3618}, doi = {10.30466/vrf.2018.93751.2264}, abstract = {Sheep keepers need suitable strategies to improve animal immunity and the quality of their products. This study was aimed to evaluate the effect of nano-selenium (nano-Se) and conjugated linoleic acid (CLA) on an antioxidant statue, trace minerals, and mRNA expression of glutathione peroxidase 1 (GPX1) and selenoprotein W1 (SEPW1) genes in the liver and peroxisome proliferator-activated receptor-gamma (PPARγ) and stearoyl COA desaturase 1 (SCD1) genes in fat- tail of male Moghani lambs. Thirty male Moghani lambs, three months old and average weight 30.00 ± 0.25 kg, were assigned to a completely randomized design in a 2×3 factorial arrangement with dietary supplementation of nano-Se (0, 1.00 and 2.00 mg kg-1 dry matter) and CLA (0 and 15.00 g kg-1 dry matter). The lambs were slaughtered at the end of the experiment, on day 90 of the experiment. Results showed that dietary inclusion of nano-Se significantly improved antioxidant enzymes glutathione peroxidase and superoxide dismutase in blood, however, did not show any differences in trace mineral treatments. The analysis of qPCR showed that nano-Se inclusion at the highest level (2.00 g kg-1 dry matter) enhanced gene expression of GPX1 (0.64 vs 0.34) and SEPW1 1 (0.72 vs 0.35) in the liver. Dietary inclusion of CLA increased the expression of PPARγ (0.63 vs 0.38) and decreased SCD1 (0.63 vs 0.33) genes in fat- tail. It could be concluded that selenium inclusion in the growing lamb’s diet could improve antioxidant status, however, no synergistic interaction was observed along with CLA on the mentioned parameters.}, keywords = {Antioxidant enzymes,‎ Diet supplementation,‎ Gene expression,‎ Glutathione peroxidase,‎ Selenium}, url = {https://vrf.iranjournals.ir/article_45643.html}, eprint = {https://vrf.iranjournals.ir/article_45643_74bfbdf70f4c8e1f201ba1a79c6d8c63.pdf} }