Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 12 2 2021 06 15 In vitro evaluation of binding capacity of different binders to adsorb aflatoxin 211 215 243057 10.30466/vrf.2019.99431.2369 EN Mohammad Hojati Department of Animal and Poultry Sciences, College of Abouraihan, University of Tehran, Iran Mohammad Ali Norouzian Department of Animal and Poultry Sciences, College of Abouraihan, University of Tehran, Iran Ali Assadi Alamouti Department of Animal and Poultry Sciences, College of Abouraihan, University of Tehran, Iran Ahmad Afzalzadeh Department of Animal and Poultry Sciences, College of Abouraihan, University of Tehran, Iran Journal Article 2018 12 11 This study was conducted to compare the efficacy of different feed additives as mycotoxin binders <em>in vitro</em>. Four prevalent aflatoxin-sequestering agents (SAs) including two bentonite clays (common and acid activated bentonite), a yeast cell wall product and an activated charcoal product were evaluated <em>in vitro</em> to verify their capacity for binding aflatoxin B1 (AFB1). The SAs were individually mixed at two different ratios with AFB1 (1:70,000, 1:120,000) and their binding capacity indices were determined. Experimental bentonites showed high adsorption abilities, binding more than 70.00% of the available AFB1. At the 1:70,000 and 1:120,000 aflatoxin binder (AF:B) ratios, acid activated bentonite were sequestered over 87.00 and 99.00% of the AFB1, respectively. Yeast cell wall showed moderate adsorption ability at the 1:120,000 AF:B ratio, adsorbing 47.00 of AFB1. The adsorption ability of activated carbon at two AF:B ratio and yeast cell wall at 1:70,000 AF:B ratio were significantly lower than other binders. The ratio of chemisorption and binding equivalency factor were higher for acid activated bentonite compared to other sequestering agents. Based on the result of this study, it seems that acid activated bentonite could be considered efficient at sequestering the available AFB1, resulting as promising agents for use in animals diet. https://vrf.iranjournals.ir/article_243057_0f71e362ff2addc61b170bbc30465869.pdf