Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran 75 80 24328 EN Ali Gilani PhD Candidate, Department of Food Hygiene and Quality Control, Science and Research Branch, Islamic Azad University, Tehran, Iran Vadood Razavilar Department of Food Hygiene and Quality Control, Science and Research Branch, Islamic Azad University, Tehran, Iran Nordahr Rokni Rokni Department of Food Hygiene and Quality Control, Science and Research Branch, Islamic Azad University, Tehran, Iran Ebrahim Rahimi Department of Food Hygiene and Quality Control, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Journal Article 2017 02 12 Foods with animal origins play a substantial role in the transmission of <em>Helicobacter pylori</em>. The present investigation was carried out to study the <em>vacA</em> and <em>cagA</em> genotypes status of <em>H. pylori</em> isolated from various types of meat samples. Two hundred and twenty meat samples were collected and cultured. <em>H. pylori</em>-positive strains were analyzed for the presence of <em>vacA</em> and <em>cagA</em> genotypes. Eleven out of 220 (5.00%) samples were positive for <em>H. pylori</em>. Findings were confirmed by nested PCR. Prevalence of <em>H. pylori</em> in the meat samples of slaughterhouses and butcheries were 72.20% and 27.70%, respectively. The most commonly detected genotypes in the meat samples of slaughterhouses and butcheries were <em>vacA</em> <em>m1a</em> (66.66%) and <em>vacA s1a</em> (37.50%), respectively. The <em>S1am1a</em> was the most commonly detected genotype. Meat sampled from butcheries had the higher prevalence of <em>H. pylori</em> and its genotypes than those of slaughterhouses (<em>p</em> < 0.05). Results showed that meat samples could be the potential sources of virulent strains of <em>H. pylori</em>. Application of sanitary measures in the storage, transportation and sale of meat is essential for reducing the levels of <em>H. pylori</em> cross contamination. https://vrf.iranjournals.ir/article_24328_588fad4e41a1544c03e7d5625c37cbf0.pdf