Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 12 3 2021 09 15 An accurate, rapid and simple loop-mediated isothermal amplification ‎method for Explanatum explanatum detection in animals 377 381 244416 10.30466/vrf.2020.111872.2657 EN Muhammad Rashid Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of ‎Karachi, Karachi, Pakistan‎ 0000-0002-6665-618X Muhammad Imran Rashid Department of Parasitology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan 0000-0002-0280-9335 Qasim Ali Department of Parasitology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan 0000-0001-5783-9630 Journal Article 2019 07 24 Paramphistomosis is an infectious disease caused by the liver flukes and it is associated with heavy loss of ruminant's population. <em>Explanatum explanatum</em> is a digenetic trematode commonly affecting domesticated ruminants. The available methods for pathogen detection are laborious and expensive and offer limited specificity; thus, considered not suitable for post mortem pathogen detection, surveillance and prevalence studies. New detection techniques offering simplicity, specificity and rapidity are absolutely needed. We have designed a loop-mediated isothermal amplification (LAMP) based polymerase chain reaction method, targeting a sequence of the <em>explanatum</em> species, using a primer pair from the internal transcribed spacer-2 region. The DNA from adult flukes belonging to <em>explanatum</em> species was isolated from infected livers and used to optimize the LAMP assay. The specificity and sensitivity of the LAMP assay were evaluated and found highly efficient in species-specific DNA detection with the sensitivity to detect 50.00 pg DNA in a 25.00 µL reaction mix. The procedure has the potential to be adapted for stool samples for field detection and disease surveillance/prevalence in rural and unprivileged areas. Explanatum explanatum liver flukes ‎ Loop-mediated isothermal ‎amplification https://vrf.iranjournals.ir/article_244416_67223ce1f5c8955db45a35067b1087f2.pdf