Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 13 3 2022 09 15 Glucose-independent insulin and glucagon secreted from ventral pancreas sharing a similar pattern in healthy adult rat 309 313 252997 10.30466/vrf.2021.138754.3087 EN Hao Duan Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China Fei He Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China Weizhou Wang Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China Medical School, Kunming Medical University, Kunming, Yunnan, China Yanghao Wang Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China Medical School, Kunming Medical University, Kunming, Yunnan, China Chenli Yang Kunming Children’s Hospital, Kunming, Yunnan, China Yihan Lin HKU-Pasteur Research Pole, Hong Kong, China Ming Li Department of Physiology, School of Medicine, Tulane University, New Orleans, USA You Lu Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China Department of Pathology and Laboratory Medicine, Tulane University, New Orleans, USA 0000-0002-6815-6568 Journal Article 2020 10 27 Elevated blood glucose concentration due to food intake will trigger insulin secretion from the dorsal pancreas has been extensively studied. This increased intracellular insulin level can stimulate glucagon release from intra-islets. However, the interaction between glucagon and insulin under a fasting state is unknown. To explore the relationship, we partially removed the ventral and dorsal pancreas on wild-type adult rats. The glucose tolerance test was conducted to measure influence of the surgery on the integrity function of the pancreas. The fasting insulin/glucagon level before and after surgery were measured by the ELISA kit. The statistical analyses indicated that the ventral removal of the pancreas had higher fasting glucose than that of dorsal removal. And only the ventral removal group showed significantly increased basal insulin and basal glucagon levels. Our findings showed differential role of the ventral pancreas in response to a glucose-free stimulus and also provided the possible <em>in vitro</em> target for developing the anti-hyperglycemic drugs. https://vrf.iranjournals.ir/article_252997_b1c4b0902d38f83767bbb4899458d87f.pdf