Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 13 3 2022 09 01 Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata 409 415 253008 10.30466/vrf.2021.529714.3174 EN Farhad Tavarideh Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran Fazel Pourahmad Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran 0000-0003-0342-1735 Mostafa Nemati Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran 0000-0002-6042-082X Journal Article 2021 05 08 To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of <em>Scrophularia striata</em> was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of <em>S. striata</em>. Using sequence analysis targeting 16S rRNA gene, eight genera, including <em>Agrococcus</em>, <em>Arthrobacter</em>, <em>Bacillus</em>, <em>Chryseobacterium</em>, <em>Delftia</em>, <em>Kocuria</em>, <em>Pseudomonas</em> and <em>Sphingomonas</em> were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against <em>Bacillus cereus</em>, 17(54.84%) isolates exhibited maximum activity against <em>Staphylococcus aureus</em>, 14(45.16%) isolates against <em>Escherichia coli</em> and 5(16.13%) isolates showed positive activity against <em>Proteus mirabilis</em>.The results obtained in this study suggested that the medicinal plant, <em>S. striata</em>is is a potent source of endophytic bacteria with antibacterial activity and offers promise for discovery of more impressive biological compounds. https://vrf.iranjournals.ir/article_253008_566609296eb4971cbc6fbb2b6fc48e2f.pdf