Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 13 4 2022 12 01 Genotypic and phenotypic tetracycline-based properties of Trueperella pyogenes isolates from bovine samples 469 474 254651 10.30466/vrf.2021.138938.3090 EN Ozlem Şahan Yapicier Bacteriological Diagnostic Laboratory, Veterinary Control Central Research Institute, Republic of Turkiye Ministry of Agriculture and Forestry, Ankara, Turkiye Dilek Ozturk Department of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkiye 0000-0002-9643-8570 Mehmet Kaya Department of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkiye Journal Article 2020 10 29 The purpose of this study was to investigate the tetracycline resistance in Trueperella pyogenes isolates from bovine samples in Burdur, Turkiye, and assess 16 tetracycline-resistance genes distribution among the isolates. Forty-nine T. pyogenes isolates were phenotypically characterized for anti-microbial resistance to doxycycline, oxytetracycline and tetracycline by disc diffusion method. Presence of tetracycline genes of T. pyogenes was investigated by multiplex and singleplex polymerase chain reaction. Our results indicated that 87.80% and 42.86% of the isolates were resistant to tetracycline and oxytetracycline, respectively, and the rate of resistance to doxycycline was 6.12%. Total of 21 (42.85%) were carrying tetracycline-resistance genes and tet(A) was present in 12 (24.49%) isolates; whereas, the tet(W) gene was identified in 9 (18.37%) and 2 (4.08%) of the isolates carried both tet(A) and tet(W), respectively. The study indicated antibiotic resistance patterns of tetracycline agents and links to the tet-genes among T. pyogenes were detected. It makes it worthwhile that this is the first report for detection of tet(A) gene in T. pyogenes.

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