Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 2 2017 06 15 Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression 89 96 25843 EN Ezzatollah Fathi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Raheleh Farahzadi Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Reza Rahbarghazi Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Hossein Samadi Kafil Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Rahman Yolmeh Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran Journal Article 2017 06 14 Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO₄) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO₄ and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO₄); group III (ZnSO₄ treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO₄). In the presence of different concentrations of ZnSO₄,cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO₄. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO₄ could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO₄ may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression. Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO₄) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO₄ and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO₄); group III (ZnSO₄ treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO₄). In the presence of different concentrations of ZnSO₄,cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO₄. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO₄ could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO₄ may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression. https://vrf.iranjournals.ir/article_25843_0e9a570f97a6f2a4e1326a10228176a4.pdf