Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 4 2017 12 15 Effect of Satureja khuzestanica essential oil against fertility disorders induced by busulfan in female mice 281 286 28477 EN Abbas Ahmadi Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 0000-0002-3201-5480 Saleh Bamohabat Chafjiri Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Rajab Ali Sadrkhanlou Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Journal Article 2017 01 21 Busulfan is an alkylating agent affects ovarian follicles growth by oxidative stress induction. <em>Satureja khuzestanica</em> has antioxidant effects. The aim of this study was to examine whether <em>S. khuzestanica</em> essential oil (SKEO) exhibits protective effects on busulfan-induced ovarian failure. Eighty-four adult female mice were divided into six groups including dimethyl sulfoxide (control), SKEO 225.00 mg kg^-1 (orally), busulfan 3.00 mg kg^-1 (orally), busulfan 36.00 mg kg^-1 (intraperitoneally), busulfan 3.00 mg kg^-1 and SKEO and busulfan 36.00 mg kg^-1 and SKEO. After 28 days, the mice were euthanized and oocytes were removed for <em>in vitro</em> fertilization (IVF) rate evaluation. Oocyte quantity and quality, fertilization rate and pre-implantation embryo development were daily examined with a stereo microscope in a period of 120 hr. Serum levels of estradiol and progesterone were also evaluated. Busulfan caused significant decreases in oocyte number and quality, fertilization rate, pre-implantation embryo development and embryo quality. The SKEO significantly decreased the adverse effects of busulfan. The present study indicated that SKEO can protect female fertility potential against busulfan induced damages. https://vrf.iranjournals.ir/article_28477_d56af33e24d2a02c40a53b26fde2bc4a.pdf