Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 9 3 2018 09 15 Royal jelly protects male mice against nicotine-induced reproductive failure 231 238 32088 10.30466/vrf.2018.32088 EN Farnam Azad Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Vahid Nejati Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Ali Shalizar-Jalali Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Gholamreza Najafi Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Fatemeh Rahmani Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Journal Article 2018 08 15 This study evaluated the possible protective effect of royal jelly (RJ) on sperm parameters and sperm malondialdehyde (MDA) concentration and in vitro fertilizing potential in nicotine (NIC) exposed male mice. Thrtiy-six male BALB/c mice were randomly divided into six groups (n = 6). Group 1 received normal saline, group 2 received 100 mg kg-1 per day RJ, groups 3 and 4 received NIC at doses of 0.50 and 1.00 mg kg-1 per day, respectively and groups 5 and 6 received NIC at doses of 0.50 and 1.00 mg kg-1 per day, respectively plus RJ. Caudal epididymal sperm characteristics, lipid peroxidation and in vitro fertilizing capacity and embryo development were evaluated after 35 days. The NIC treatment caused a significant decrease in sperm motility and viability and fertilization rate along with poor blastocyst formation and increased sperm DNA damage and MDA levels. Moreover, the incidences of chromatin abnormality in spermatozoa were significantly higher in NIC-exposed mice than those of control. Nevertheless, RJ treatment improved sperm parameters and in vitro fertilization outcome as well as sperm lipid peroxidation level. Data from the current study suggest that RJ has a potential repro-protective action against NIC-induced sperm abnormalities and embryotoxicity in mice.

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