Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 15 1 2024 01 01 Immediate administration of hTERT-MSCs-IDO1-EVs reduces hypoalbuminemia after spinal cord injury 27 34 709907 10.30466/vrf.2023.2003942.3903 EN Shiva Amanollahi Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Ahmad Reza Bahrami Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran Industrial Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran 0000-0001-9122-7216 Azadeh Haghighitalab Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran Stem Cells and Regenerative Medicine Research Group, Academic Center for Education, Culture and Research (ACECR), Mashhad, Iran 0000-0001-6005-4156 Haniyeh Shaterzadeh Yazdi Private Veterinary Practitioner, Mashhad, Iran Hossein Kazemi Mehrjerdi Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 0000-0002-5593-6864 Journal Article 2023 06 09 Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery rate and the efficiency of treatments following SCI. In this study, the effect of extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the human <em>indoleamine 2,3-dioxygenase-1</em> (<em>IDO1</em>) gene on serum albumin and glucose levels was investigated. After pre-clearing steps of 72-hr conditioned media, small EVs (sEVs) were isolated based on the ultra-filtration method. They were encapsulated with a chitosan-based hydrogel. Five experimental groups (female rats, N = 30, ~ 230 g) were considered, including SCI, sham, hydrogel, control green fluorescent protein (GFP)-EVs and <em>IDO1</em>-EVs. The 60.00 µL of hydrogel or hydrogels containing 100 µg sEVs from GFP or <em>IDO1</em>-EVs were locally injected immediately after SCI (laminectomy of the T10 vertebra and clip compression). After 8 weeks, non-fasting serum glucose and albumin levels were measured. The results indicated that the level of serum albumin in the animals received <em>IDO1</em>-EVs (3.52 ± 0.04) was increased in comparison with the SCI group (3.00 ± 0.94). Also, these animals indicated higher glucose levels in their serum (250.17 ± 69.61) in comparison with SCI ones (214 ± 45.34). Although these changes were not statistically significant, they could be considered as evidence for the beneficial effects of <em>IDO1</em>-EVs administration in the context of SCI to reduce hypoalbuminemia and improve energy consumption. More detailed experiments are required to confirm these results. https://vrf.iranjournals.ir/article_709907_3290515f04f4d8c9bb26d5e93d717ac6.pdf