Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 15 6 2024 06 01 Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt 267 274 712793 10.30466/vrf.2023.2007392.3946 EN Mohamed Mohamed Ali Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt Salwa Mahmoud Helmy Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt Hanan Ali Fahmy Department of Biotechnology, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt Haitham Elaadli Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt Ibrahim Elsayed Eldesoukey Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt 0000-0003-4172-1210 Journal Article 2023 07 19 <em>Staphylococcus aureus</em> is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of <em>S. aureus</em> in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for <em>S. aureus</em> using both conventional culture-based and molecular-based methods. Antibiotic susceptibility testing of <em>S. aureus</em> isolates was conducted using disc diffusion and agar dilution methods, with antibiotic resistance genes identified through polymerase chain reaction with specific primers. Out of samples tested, 60 (30.00%) were positive for <em>S. aureus</em>. The isolates displayed the highest of resistance against piperacillin-tazobactam (55.00%) followed by trimethoprim- sulfamethoxazole (45.00%) and amoxicillin (40.00%). Half of the isolates were multidrug-resistant (MDR). The AMR genes included methicillin-resistant gene (<em>mecA</em>), β-lactamase gene (<em>blaZ</em>), tetracycline resistance gene (<em>tetK</em>), erythromycin resistance gene (<em>ermB</em>) and vancomycin resistant gene (<em>vanA</em>) were detected in 100%, 100%, 95.00%, 90.00% and 20.00% of the isolates, respectively. In conclusion, the presence of MDR<em>S aureus</em> as a cause of clinical camel mastitis is a significant veterinary and public health concern. These findings highlight the importance of proper antibiotic use in Egyptian camel farms and the need for molecular techniques to fully understand the genetic profile of antimicrobial-resistant <em>S. aureus</em> isolates. https://vrf.iranjournals.ir/article_712793_763edffef9830d6029aaba3c29815589.pdf