Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 17 1 2026 01 01 Rotavirus infection in cattle 63 65 730128 10.30466/vrf.2025.2044037.4506 EN Yahia Hassan Ali Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia 0000-0003-4132-4020 Intisar Kamil Saeed Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia 0000-0003-0554-021X Muaz Magzoub Abdellatif Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia 0000-0001-5093-7262 Amani Ahmed Ali Department of Pharmacology and Toxicology, College of Pharmacy, Northern Border University, Arar, Saudi Arabia Alaa Ahmed Mustafa Department of Mathematics, College of Science, Northern Border University, Arar, Saudi Arabia Hind Abdel-Mageed Rikabi Department of Pharmacy Practice, College of Pharmacy, Northern Border University, Arar, Saudi Arabia Husham Mohammed Al-Hasssan Ataalfadeel Department of Mathematics, College of Science, Northern Border University, Arar, Saudi Arabia 0000-0001-7216-167X Journal Article 2024 10 28 Rotavirus is one of the major causes of diarrhea in different animal species and has a bad economic impact due to the losses in neonates and productivity. To investigate the occurrence of this infection in bovine calves, three localities in Khartoum State, Sudan, were selected. A total of 200 fecal samples were collected from diarrheic calves; 100 from Khartoum and 50 from each of Khartoum Bahari and Omdurman provinces. Collected samples were screened for a group A rotavirus antigen using enzyme-linked immunosorbent assay (ELISA). Positive results were seen in 40.00% of samples; the highest prevalence of 42.00% was found in samples from Khartoum province. Five ELISA-positive samples were examined under electron microscope, and characteristic wheel-like appearance of rotavirus was visualized. Polyacrylamide gel electrophoresis was also applied on 15 of the positive samples; eight samples showed different polyacrylamide gel electrophoretic group A rotavirus long profile with different patterns. The results showed that the occurrence of rotavirus infection in cattle in Khartoum State is increasing. https://vrf.iranjournals.ir/article_730128_4a95f4c21a73d40615dc388693af8114.pdf