Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 17 9 2026 09 01 Tannic acid ameliorates sperm quality and testicular damage in a rat model of ischemia-reperfusion 639 645 731723 10.30466/vrf.2025.2072773.4928 EN Hosna Ghaemi Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Ali Soleimanzadeh Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 0000-0002-1591-2198 Rahim Mohammadi Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 0000-0002-2104-9829 Ali Shalizar-Jalali Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 0000-0002-9811-7754 Journal Article 2025 09 26 This study evaluated the protective effects of intraperitoneal (IP) tannic acid (TA) against ischemia-reperfusion (IR) injury in a rat model of testicular torsion. Eighteen mature male Wistar rats were randomized into three groups, including sham (surgery without ischemia), IR (3 hr ischemia + 3 hr reperfusion), and IR + TA (50.00 mg kg^-1 TA; 100 µL intraperitoneally 30 min before reperfusion). Testicular tissues were sampled immediately after reperfusion for biochemical assays to measure malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels. Epididymides were analyzed 60 days later for sperm count, motility, viability, morphology, and DNA integrity. The IR significantly elevated MDA and sperm DNA damage while reducing SOD, GPx, sperm parameters, testicular weight, and spermatogenesis. The TA administration reversed these changes and restored the parameters to levels close to those of the sham group. Overall, intraperitoneal TA mitigated IR-induced oxidative stress and preserved reproductive function, indicating its potential therapeutic value in testicular torsion. https://vrf.iranjournals.ir/article_731723_43b9abbe605b3ddb929c06c4527c8f53.pdf