Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 17 1 2026 01 01 Effect of dapagliflozin on rat liver ischemia-reperfusion injury 47 55 732582 10.30466/vrf.2025.2050773.4632 EN Vahid Mahmoudi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Siamak Kazemi-Darabadi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran 0000-0003-3219-6071 Seyed Hosein Jarolmasjed Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Monireh Khordadmehr Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran 0000-0002-4472-3847 Journal Article 2025 01 15 In recent years, liver transplantation has emerged as the standard therapy for several liver disorders. Throughout the procedure, the transplanted liver tissue is subjected to varying degrees of ischemia-reperfusion (IR) damage. Consequently, there has been a long-standing pursuit of substances that can alleviate the harm caused by IR. In our investigation, we employed dapagliflozin as a potential therapeutic agent. Eighteen Wistar rats were divided into three groups (n = 6), including treatment, IR, and control that did not undergo surgical intervention. Two days prior to surgery, the treatment group received dapagliflozin at a dosage of 10.00 mg kg^-1 orally. During surgery, liver ischemia was induced for 1 hr, followed by a 24-hr reperfusion period. The IR group exhibited elevated levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, bilirubin, lactate dehydrogenase, and malondialdehyde compared to the control group. In contrast, the treatment group showed levels of these factors that were closer to those of the control group. While total protein, albumin, and total anti-oxidant capacity decreased in the IR group, this decline was less significant in the treatment group. Analysis of oxidative stress in liver tissue revealed that the treatment group had increased anti-oxidant capacity, and exhibited less oxidative stress compared to the IR group. Furthermore, dapagliflozin was found to reduce the degree of liver edema, necrosis, and vascular hyperemia following IR. Overall, dapagliflozin demonstrates the potential to lessen liver damage, enhance liver tissue regeneration, and mitigate the consequences associated with liver impairment. https://vrf.iranjournals.ir/article_732582_906876d5a90f8a8fbabe528636c20233.pdf