Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 17 2 2026 02 01 Pathological, immunohistochemical and molecular studies on Chlamydia spp. in cattle, sheep, and goat abortions 71 79 732797 10.30466/vrf.2025.2044227.4510 EN Ibrahim Deniz Bacteriology Laboratory, Konya Veterinary Control Institute, Konya, Türkiye 0000-0001-8526-8396 Ertan Oruc Department of Pathology Alaeddin Keykubat Campus, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye 0000-0003-4234-8219 Journal Article 2024 10 29 This study, aimed to investigate Chlamydia abortus, C. pecorum, and C. psittaci in bovine, sheep, and goat abortions sent to the Konya Veterinary Control Institute diagnostic laboratory from various provinces in Central Anatolia and Mediterranean regions of Turkey between 2019 and 2022 via pathological and molecular methods. For this purpose, a total number of 150 abortions (from 58 cattle, 73 sheep, and 19 goats) were analysed via quantitative real-time polymerase chain reaction, histopathology and immunohistochemistry. The chlamydial DNA positivity rate was determined to be 24.00% via quantitative polymerase chain reaction analyses. According to the animal species, the positivity rate was 45.20% in sheep and 15.78% in goats. No cases positive for chlamydial DNA were found in the cattle abortions analyzed in the present study. When chlamydial agents were analysed on a species basis, C. abortus and C. pecorum were detected in 22.66and 1.33% of the cases, respectively. Among the animal species, 42.47% were C. abortus positive, whereas, 2.74% were C. pecorum positive in sheep. In the present study, the percentage of C. abortus-positive goats was 15.78%. and 22.66% of the Chlamydia spp. were positive according to immunohistochemical staining of aborted fetal lung, liver, heart, placenta and umbilical cord samples. This comparative study revealed that C. abortus was the most common chlamydial abortion agent in Türkiye. Chlamydial abortion agents cause abortion mostly in sheep in the region according to the animal species, and immunohistochemical staining contributes to the diagnosis of the agent at the Chlamydia spp. level.

https://vrf.iranjournals.ir/article_732797_888377052dbc0fff6964047e188f08fd.pdf