Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 17 3 2026 03 01 Identification of Chlamydia abortus, Coxiella burnetii, and Brucella species from ruminant fetal abomasal contents using molecular methods 153 160 733528 10.30466/vrf.2025.2052553.4653 EN Ayfer Güllü Yücetepe Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye 0000-0002-9842-3305 Sadiye Dılşa Ateş Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye 0000-0003-2740-7313 Osman Yaşar Tel Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye 0000-0001-7848-3899 Özden Dellal Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye Oktay Keskin Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye 0000-0002-5977-7872 Ahmet Murat Saytekin Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye 0000-0001-7486-8054 Sibel Kızıl Department of Microbiology, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Türkiye 0000-0003-0697-3092 Ufuk Ülker Department of Microbiology, Faculty of Veterinary Medicine, Bozok University, Yozgat, Türkiye 0000-0002-5295-5584 Efsun Melike Çeçen Department of Microbiology, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Türkiye 0000-0002-3237-9073 Sevil Erdenliğ Gürbilek Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye 0000-0002-0377-2650 Journal Article 2025 02 04 This study aimed to detect Coxiella burnetii, Chlamydia abortus, and Brucella species in the abomasal contents of aborted ruminant fetuses from the Central Anatolia region of Türkiye using PCR between 2020 and 2023. The abomasal contents of a total of 97 aborted fetuses from cattle, sheep, and goats with a history of abortion, collected between the years 2020 and 2023, were tested in this study. As a result of PCR analysis of 97 abomasal contents, four (4.10%; 95.00% confidence interval [CI]: 1.33 - 10.82) of them were C. abortus, including three sheep and one goat. Two (2.10%; 95.00% CI: 0.36 - 7.96) of them were C. burnetii, including one sheep and one cow. A total of 60 (61.90%; 95.00% CI: 51.40 - 71.37) samples from 47 cattle, nine sheep, and four goats were determined by Brucella genus-specific PCR. Following multiplex PCR analysis of the positive Brucella spp. samples, 39 (65.00%; 95.00% CI: 51.52 - 76.55) samples were identified as B. abortus, including two sheep, one goat, and 36 cattle. Additionally, 19 (31.70%; 95.00% CI: 20.60 - 45.09) isolates were identified as Brucella melitensis, including five sheep, two goats, and 12 cattle. In two sheep samples, both B. melitensis and C. abortus were identified from the same animals. In conclusion, Brucella spp. were the predominant abortion-causing pathogens, with C. abortus also contributing significantly. Effective control strategies under the One Health approach are essential to prevent the uncontrolled spread and inter-species transmission of these zoonotic agents in the region and country.