Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Evaluation of the morphological features of the uterine tubes during postnatal development in West African Dwarf goats (Capra hircus) 1 6 24320 EN Clifford Nwabugwu Abiaezute Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria. Innocent Chima Nwaogu Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria Udensi Maduabuchi Igwebuike Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria Journal Article 2017 02 12 The objective of this study was to highlight the postnatal development of the uterine tubes of the West African Dwarf goat from birth to 28 weeks of age by gross examination and light microscopy. There was a caudal migration of the paired uterine tubes from behind the paired kidneys at birth to the pelvic inlet at week 8 of age. Each uterine tube exhibited three segments namely; infundibulum, ampulla and isthmus. A marked flexure, the utero-tubal junction, was the point at which the uterine tubes joined the uterine horns. The length and absolute weight of the uterine tubes increased from 4.95 ± 0.28 cm and 0.02 ± 0.01 g at birth to 14.98 ± 2.79 cm and 0.22 ± 0.03 g at week 28 of age, respectively. The mucosa of the infundibulum and the ampulla showed long, branched and anastomosing primary, secondary and tertiary mucosal folds which decreased in height towards the isthmus. The mucosal folds within the isthmus were short and lacked the anastomosing pattern. The epithelia of all three segments were pseudostratified columnar. Numerous secretory blebs and extruded nuclei became apparent from week 16 of age. The thickness of the tunica muscularis varied with the segments. https://vrf.iranjournals.ir/article_24320_b2f29338576d3ba925ba0f99088726b5.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Effect of extracellular matrix on testosterone production during in vitro culture of bovine testicular cells 7 13 24314 EN Vahid Akbarinejad Young Researchers and Elites Club, Roudehen Branch, Islamic Azad University, Roudehen, Iran Parviz Tajik Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mansoureh Movahedin Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Reza Youssefi Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2017 02 12 Testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. Given that extracellular matrix (ECM) facilitates differentiation of spermatogonial stem cells (SSCs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ECM on SSCs differentiation. In experiment 1, testosterone production was measured in testicular cells cultured for 12 days on ECM or plastic (control). In experiment 2, testosterone production was assessed in testicular cells cultured on ECM or plastic (control) and exposed to different concentrations of hCG. In experiment 3, the gene expression of factors involved in testosterone production was analyzed. Testosterone concentration was lower in ECM than in the control group in experiment 1 (<em>p</em> < 0.05). In experiment 2, testosterone concentration was increased in response to hCG in both groups but cells cultured on ECM were more responsive to hCG than those cultured on plastic (<em>p</em> < 0.05). In the experiment 3, qRT-PCR revealed the inhibitory effect of ECM on the gene expression of steroidogenic acute regulatory protein (StAR) (<em>p </em>< 0.05). Nevertheless, the expression of LH receptor was greater in ECM-exposed than in unexposed cells (<em>p</em> < 0.05). In conclusion, the present study showed that inhibiting the expression of StAR, ECM could lower testosterone production by Leydig cells during <em>in vitro</em> culture. In addition, the results indicated that ECM could augment the responsiveness of Leydig cells to hCG through stimulating the expression of LH receptor. https://vrf.iranjournals.ir/article_24314_70e3da32d14d879905434986d67aa833.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Characterization of isolated pigeon paramyxovirus-1 (PMV-1) and its pathogenicity in broiler chickens 15 21 24315 EN Mansoor Mayahi Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Masoud Reza Seyfi Abad Shapouri Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Ramezan Ali Jafari Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Mehrdad Khosravi Farsani DVSc Graduate of Avian Health and Diseases, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Journal Article 2017 02 12 Characterization of isolated pigeon paramyxovirus-1 (PMV-1) and its pathogenicity in broiler chickens were studied. Two hundred and thirty-two samples collected from 50 unvaccinated pigeons lofts suspected to Newcastle disease from private houses and bird markets from Ahvaz‌, Iran. Swab samples from cloaca and oropharynx of live pigeons and from trachea, lung, liver, spleen, kidney, brain, proventriculus and cecal tonsil of dead pigeons suspected to ND were collected. Isolation of the PPMV-1 was performed through intra-allantoic inoculation of 9- to 11- day-old embryonated chicken eggs. The RNA extraction and cDNA synthesis were conducted. With PCR, multiplication of cleavage site of F gene was carreid out and PCR products were sequenced and phylogenetic comparison on isolates was performed. For pathogenecity study of isolated PPMV-1, one hundred sixty day-old broiler chicks were divided into four equal groups. Groups 1 and 2 chicks vaccinated against ND by B1 vaccine at nine days. Groups 3 and 4 were kept as unvaccinated control groups. Groups 1 and 4 chicks were challenged with 10⁵EID₅₀of highest virulent isolated PPMV-1 by ocular route at day 29. The results indicated PPMV-1 is enzootic in Ahvaz pigeons and all isolates were virulent Newcastle disease virus with 112KRQKR*F117 motif. For study pathogenicity of pigeon isolate in chickens, they challenged with most virulent isolate, showed respiratory signs, conjunctivitis and in some cases depression and lethargy. In conclusion, isolated PPMV-1 is a virulent NDV and can infect chickens and produce mild ND in unvaccinated chickens. https://vrf.iranjournals.ir/article_24315_ca0912c01f24c6ef488db9a2a97b7768.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Effects of silver nanoparticles on Staphylococcus aureus contaminated open wounds healing in mice: An experimental study 23 28 24835 EN Masood Adibhesami PhD candidate, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Malahat Ahmadi Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Amir Abbas Farshid Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Farshid Sarrafzadeh-Rezaei Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 0000-0003-4692-3831 Bahram Dalir-Naghadeh Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Journal Article 2017 03 14 The microorganisms have been noted as the main cause of delayed wound healing.The most common pathogen causing the wound infections is <em>Staphylococcus aureus</em>. Silver nanoparticles (AgNPs) show ample antibacterial activities. In the present study, the effect of AgNPs on mouse wounds inoculated with <em>S. aureus</em> was investigated. Sixty male mice (20 to 30 g) were anesthetized, full-thickness skin wounds were made on their back and then the bacterial suspension was added to each wound bed. Treatments were administered on wound bed topically including gentamicin (8 mg kg^-1), AgNPs (0.08 mg kg^-1, 0.04 mg kg^-1 and 0.02 mg kg^-1) and normal saline in the control group. Wound healing was monitored macroscopically by taking digital photographs on days 0, 7, 14 and 21 of the experiment. Topical application of gentamicin and AgNPs (0.08 and 0.04 mg kg^-1) significantly increased the rate of wound healing more than treatment with AgNPs at a dose of 0.02 mg kg^-1and normal saline. The presence of silver nanoparticles in AgNPs groups (especially 0.08 mg kg^-1) improved wound appearance better than other groups without silver nanoparticles (gentamicin and control groups) and led to lesser wound scars. According to data analysis, healing rate of treated mice with gentamicin and AgNPs (0.08 mg kg^-1) was significantly (<em>p </em>< 0.001) faster than treated mice with other AgNPs doses and normal saline. The results of current study introduced an <em>in vivo</em> nanosilver accelerating effects on the treatment of on <em>S. aureus</em> infected skin wounds. https://vrf.iranjournals.ir/article_24835_2842db40fd1da44e0be74335fb55004d.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Role of μ-opioid receptor in parafascicular nucleus of thalamus on morphine-induced antinociception in a rat model of acute trigeminal pain 29 34 24322 EN Esmaeal Tamaddonfard Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 0000-0001-8240-5885 Amir Erfanparast Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Journal Article 2017 02 12 The parafascicular nucleus (PFN) of thalamus, as a supraspinal structure, has an important role in processing of nociceptive information. In addition, μ-opioid receptor contributes to supraspinal modulation of nociception. In the present study, the effects of microinjection of naloxone (a non-specific opioid-receptor antagonist) and naloxonazine (a specific μ-opioid receptor antagonist) were investigated on morphine-induced antinociception in a rat model of acute trigeminal pain. Right and left sides of PFN of thalamus were implanted with two guide cannulas. Acute trigeminal pain was induced by local corneal surface application of hypertonic saline and the number of eye wipes as a pain index was recorded for 30 sec. Microinjection of morphine at doses of 1, 2 and 4 μg per site significantly (<em>p</em> < 0.05) decreased the number of eye wipes. Alone microinjection of naloxone (4 μg per site) and naloxonazine (1 and 2 μg per site) significantly (<em>p</em> < 0.05) increased corneal pain severity. Prior microinjection of naloxone (2 and 4 μg per site) and naloxonazine (1 and 2 μg per site) significantly (<em>p</em> < 0.05) prevented the antinociceptive effect induced by morphine (4 μg per site). All the above-mentioned chemicals did not alter locomotor behavior in an open-field test. The results of the present study showed an antinociceptive effect of morphine at the PFN level of thalamus. Mu-opioid receptor of the PFN of thalamus may be involved in morphine-induced antinociception. https://vrf.iranjournals.ir/article_24322_b13c180ba3b4c0afdd38ed2cb91fc498.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Pathogenicity study of Iranian genotype of avian infectious bronchitis virus (IR-1) 35 41 24318 EN Hamideh Najafi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 0000-0001-5329-5808 Arash Ghalyanchi-langeroudi Department of Microbiology and immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran 0000-0001-7914-0863 Masoud Hashemzadeh Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran Vahid Karimi Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Omid Madadgar Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Reza Khaltabadi Farahani Iranian veterinary Organization, Tehran, Iran Seyed Ali Ghafouri Iranian veterinary Organization, Tehran, Iran Hossein Maghsoudloo Iranian veterinary Organization, Tehran, Iran Parvaneh Seifouri Iranian veterinary Organization, Tehran, Iran Ali Madhi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2017 02 12 Avian infectious bronchitis (IB) is a major cause of economic losses in poultry industry. The IB virus primarily affects respiratory tract, but various strains differ in their tropism for other target organs such as kidney and alimentary tract. The objective of this study was to estimate the pathogenicity of Iranian IBV variant (IR-1), which is limited exclusively to Iran. Specific pathogen free chicks were inoculated intranasally. Sera, fecal swabs and different tissue samples were collected on different days post infection (DPI). Clinical signs, gross pathology and histological changes were recorded. The viral load was quantified in the RNA extractions from different tissue samples using real-time PCR. Anti-IBV antibodies were detected in serum samples. The IgG antibody were found on 21 and 28 DPI. Severe histological lesions were observed in the trachea and lung while the lesions in kidney were appeared to be milder. Viral RNA was detected in all tested tissues from 1 DPI to the last day of the experiment. The highest viral load was measured in the trachea and feces on 1^st and 5^th DPI, respectively. It can be concluded the IR-1 had broad tropism for respiratory tract, digestive system, and renal tissue, reflecting its epitheliotropic nature, but it caused the most severe lesions in the respiratory tract. This was the first pathogenicity study of Iranian IR-1 IBV. Further knowledge of IBV pathogenesis provides the groundwork to inform more effective prevention practices. https://vrf.iranjournals.ir/article_24318_9154498493d8e734d9c7489c2b6b26d7.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Changes in the expression of OCT4 in mouse ovary during estrous cycle 43 48 24317 EN Narges Bagheripour Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran Saeed Zavareh Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran Institute of Biological Sciences, Damghan University, Damghan, Iran Mohammad Taghi Ghorbanian Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran Institute of Biological Sciences, Damghan University, Damghan, Iran Seyed Hassan Paylakhi Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran Institute of Biological Sciences, Damghan University, Damghan, Iran Seyed Reza Mohebbi Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Article 2017 02 12 The transcriptional factor OCT4 regulates pluripotency of stem cells and has an important role during oocyte growth. Whereas, its role has remained ambiguous in ovarian tissue during reproductive cycle. Therefore, this study was aimed to investigate the expression patterns of OCT4 in mouse ovaries during the normal estrous cycle. Adult National Medical Research Institute mice were classified as proestrous, estrous, metestrous and diestrous on the basis of vaginal smear cytology. Their ovaries were removed and the protein and gene expression levels of OCT4 were assessed using immunohistochemical staining and real-time quantitative reverse-transcription PCR, respectively. Immunohistochemical staining revealed the expression of OCT4 in the cytoplasm of corpus luteum cells. In the follicles, OCT4 was expressed in the cytoplasm of granulosa cells. Furthermore, the gene expression levels of OCT4 was significantly higher in the proestrous phase than in the other phases of the estrous cycle (<em>p </em>< 0.05). The results indicated that OCT4 gene expression levels are affected by the cyclic pattern of the estrous cycle. https://vrf.iranjournals.ir/article_24317_14318e2a43a225fc24ab55f3c0122cf0.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Determination of the optimal enrichment Artemia franciscana with a synbiotic combination of probiotics Pediococcus acidilactici and prebiotic fructooligosaccharide 49 54 24316 EN Mahmood Azimirad Department of Fishery, Faculty of Natural Resources, Urmia University, Urmia, Iran Saeed Meshkini Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Journal Article 2017 02 12 In this study the optimal amount of enriching <em>Artemia franciscana</em> with a synbiotic combination of <em>Pediococcus acidilactici</em> and fructooligosaccharide (FOS( was investigated. The experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics <em>P. acidilactici</em> (1×10⁹, 1×10⁸ and 1× 10⁷ CFU per L) and multi-level enriched prebiotic FOS (1, 2 and 5) g per L of solution and control. To evaluate the enrichment of adult artemia with each treatment, sampling was performed at 2, 4 and 6 hr after initiation of enrichment. The results showed that a synbiotic containing a probiotic treatments <em>P. acidilactici</em> at 1 × 10⁹ and <br /> 1 × 10⁸ CFU per L had more bacteria than a synbiotic containing a probiotic treatment with 1 × 10⁷CFU per L (<em>p </em>< 0.05), but did not show significant difference between levels of 1 × 10⁹and 1 × 10⁸ CFU per L (<em>p </em>> 0.05). The highest number of attached bacteria (6.78 ± 0.03 log CFU g^-1) to adult artemia was shown after 6 hr of enrichment that showed significant difference with 2 hr, but did not show significant difference with 4 hr time. In conclusion, the results of this study showed that adult artemia in a short time (about 4 hr) unlike nauplii artemia can retain a large amount of probiotic (1 × 10⁸ CFU <em>P. acidilactici</em> per L and 5 g per L FOS prebiotic) on their own. https://vrf.iranjournals.ir/article_24316_70a3e3e59bc61c8eb7acfbba1073980c.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail 55 58 24319 EN Hesam Alizade Infectious and Tropical Disease Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran Reza Ghanbarpour Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran Zoonosis Research Committee, Kerman University of Medical Sciences, Kerman, Iran Maziar Jajarami Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran Asma Askari Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran Journal Article 2017 02 12 Colibacillosis caused by avian pathogenic <em>Escherichia coli</em> (APEC) is an economic threat to the poultry industry throughout the world. Some of the virulence genes may enhance the ability of <em>E. coli</em> isolates to grow in the tissues of broilers. The APEC strains are assigned to a few distinct phylogenetic groups. The purpose of the present study was to detect the virulence genes and phylogenetic groups of <em>E. coli </em>isolates from colibacillosis cases in Japanese quail in 2014 in Kerman, Iran. In the present study, one hundred and two <em>E. coli</em> isolates were obtained from dead Japanese quails with colibacillosis. <em>E. coli </em>isolates were confirmed by standard biochemical and bacteriological methods. DNA of <em>E. coli </em>isolates was extracted by boiling method. The confirmed <em>E. coli</em> isolates were investigated to detect the phylogenetic groups and virulence genes including <em>sfa/focDE</em>, <em>afaIBC</em>,<em> papEF </em>by PCR methods. <em>E. coli</em> isolates were classified into A (62 isolates), B1 (24 isolates), B2 (12 isolates) and D (four isolates) phylogenetic groups. Among examined isolates nine isolates (8.82%) were positive for <em>papE-F</em>, five isolates (4.90%) for <em>afaIB-C</em> and two isolates (1.96%) for <em>sfa/focD-E</em> genes. Based on our findings, <em>E. coli</em> isolates from colibacillosis of Japanese quail could be assigned to various phylogenetic groups (mostly A and D), and they may contain the adhesion genes in a low prevalence. https://vrf.iranjournals.ir/article_24319_62343fe688d3b70314b5c1730328a936.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Modeling of Salmonella typhimurium growth under the effects of Carum copticum essential oil, temperature, pH and inoculum size 59 65 24321 EN Golshan Shakeri Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Abdollah Jamshidi Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Saeid Khanzadi Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 0000-0003-0106-587X Mohammad Azizzadeh Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Journal Article 2017 02 12 The purpose of this study was to elucidate some factors affecting the growth of <em>Salmonella typhimurium</em>. These factors included <em>Carum copticum</em> essential oil (0%, 0.015%, 0.03% and 0.06%), temperature (25 ˚C and 35 ˚C), pH (5, 6 and 7) and inoculum size (10³and 10^{5<br />} CFU mL^-1). Brain heart infusion broth was used as the growth medium. There were 48 treatment combinations and the experiment was carried out in triplicate. Growth was monitored by visible turbidity over a 30 days period. A parametric survival model based on the log-normal distribution was used to estimate the most influential factors on the time to detection of <em>Salmonella</em> growth. According to our results, the selected factors significantly affected the growth of <em>Salmonella typhimurium</em>. Furthermore, the final graph demonstrated good agreement between the values predicted by predictive model and the results which were observed in this study. So that a parametric survival model can be a useful and practical tool to predict how the parameters will influence the bacterial growth. https://vrf.iranjournals.ir/article_24321_a774b1d228003465cbd98d456af7bf43.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Molecular characterization and phylogenetic analysis of feline hemoplasmas in domestic cats in Iran 67 73 24326 EN Fereshteh Ghazisaeedi Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Nahid Atyabi Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Taghi Zahraei Salehi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Iraj Ashrafi Tamai Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 0000-0001-6784-3721 Saeid Tabatabaei Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Solmaz Chegeni Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2017 02 12 Three known feline hemoplasmas are <em>Mycoplsama haemofelis</em>, ‘<em>Candidatus</em> <em>Mycoplasma haemominutum</em>’ and ‘<em>Candidatus</em> <em>Mycoplasma turicensis’</em>. They are described as cause of feline infectious anemia in domestic and wild felids. Other blood parasites or blood-related pathogens like concurrent retroviral infections may deteriorate the clinical condition and severity of anemia. The aims of this study were molecular characterization and phylogenetic analysis of hemoplasmas in domestic cats in Iran for the first time. Blood samples were collected from 185 healthy and diseased domestic cats. Blood smears were prepared and hematological parameters were measured to determine possible anemia. Using 16S rRNA gene universal and species specific polymerase chain reactions with the following sequencing, 47 (25.40%) of cats were hemoplasma positive. Also, 17.02%, 72.50% and 40.40% of total positive samples were <em>M.</em> <em>haemofelis</em>, ‘<em>Ca</em>. M. haemominutum’ and ‘<em>Ca</em>.M. turicensis’ infected, respectively. 10 (21.20%) of hemoplasma positive cats had anemic blood profiles (HCT < 24.00%). All M. <em>haemofelis</em> infected cases were included. Partial 16S rRNA gene phylogenetic analysis revealed a high identity between the hemoplasma species found in this study and domestic cat sequences existing in GenBank. Phylogenetic analysis revealed 94.00% to 100% sequence identity between sequences of this study and existing sequences in Genbank. All hemoplasma isolates in this study were grouped within a single clade and additionally subdivided into two groups; haemofelis group including <em>M.</em> <em>haemofelis</em> and ‘<em>Ca</em>. M. <em>turicensis’</em> and haemominutum group including ‘<em>Ca</em>. M. haemominutum’. https://vrf.iranjournals.ir/article_24326_4c8c1946e71215e6e30d5f5de3f473ef.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran 75 80 24328 EN Ali Gilani PhD Candidate, Department of Food Hygiene and Quality Control, Science and Research Branch, Islamic Azad University, Tehran, Iran Vadood Razavilar Department of Food Hygiene and Quality Control, Science and Research Branch, Islamic Azad University, Tehran, Iran Nordahr Rokni Rokni Department of Food Hygiene and Quality Control, Science and Research Branch, Islamic Azad University, Tehran, Iran Ebrahim Rahimi Department of Food Hygiene and Quality Control, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Journal Article 2017 02 12 Foods with animal origins play a substantial role in the transmission of <em>Helicobacter pylori</em>. The present investigation was carried out to study the <em>vacA</em> and <em>cagA</em> genotypes status of <em>H. pylori</em> isolated from various types of meat samples. Two hundred and twenty meat samples were collected and cultured. <em>H. pylori</em>-positive strains were analyzed for the presence of <em>vacA</em> and <em>cagA</em> genotypes. Eleven out of 220 (5.00%) samples were positive for <em>H. pylori</em>. Findings were confirmed by nested PCR. Prevalence of <em>H. pylori</em> in the meat samples of slaughterhouses and butcheries were 72.20% and 27.70%, respectively. The most commonly detected genotypes in the meat samples of slaughterhouses and butcheries were <em>vacA</em> <em>m1a</em> (66.66%) and <em>vacA s1a</em> (37.50%), respectively. The <em>S1am1a</em> was the most commonly detected genotype. Meat sampled from butcheries had the higher prevalence of <em>H. pylori</em> and its genotypes than those of slaughterhouses (<em>p</em> < 0.05). Results showed that meat samples could be the potential sources of virulent strains of <em>H. pylori</em>. Application of sanitary measures in the storage, transportation and sale of meat is essential for reducing the levels of <em>H. pylori</em> cross contamination. https://vrf.iranjournals.ir/article_24328_588fad4e41a1544c03e7d5625c37cbf0.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 01 Leydig cell tumor in ovary of a German Shepherd bitch: An immunohistopathological study 81 84 24323 EN Ghasem Farjanikish Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorram Abad, Iran 0000-0002-3060-6439 Ahmad Oryan Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Journal Article 2017 02 12 Leydig cell tumor as a sex-cord stromal tumor is a relatively uncommon ovarian tumorin bitch. A 10-year-old female German Shepherd dog was presented because of protrusion of a large tumor-like mass of 16 × 14 × 7 cm in dimensions from her vagina. After stabilization of the patient, the mass was removed surgically and concurrent ovariohysterectomy was also performed. Macroscopically, the healthy tissue of the right ovary was totally replaced by a homogeneous, brown and firm mass. The neoplasm was well-circumscribed and nodular and it was clearly demarcated from the healthy tissue. Histological examination revealed the presence of solid sheets and acinar structures composed of polyhedral to elongated cells. The neoplastic cells had large, eosinophilic, and vacuolated cytoplasms with round to oval nuclei and expressed vimentin on immunohistochemical examination. These gross, microscopic and immuno-histochemical features are characteristics of ovarian Leydig cell tumor. https://vrf.iranjournals.ir/article_24323_a6a87ed8d5af6aeff8d859ffd50c5db0.pdf

Faculty of Veterinary Medicine, Urmia University Veterinary Research Forum 2008-8140 8 1 2017 03 15 Polymelia with unhealed navel in an Iranian indigenous young fowl 85 87 24327 EN Belal Hassanzadeh Central Lab, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Arefeh Rahemi Navid Institute of Radiology and Sonography, Tabriz, Iran Journal Article 2017 02 12 Developing supernumerary limbs is a rare congenital condition that only a few cases have been documented. Depending on the cause and developmental conditions, they may be single, multiple or complicated, and occur as a syndrome or associated with other anomalies. Polymelia is defined as the presence of extra limb(s) which have been reported in human, mouse, chicken, calf and lamb. It seems that the precise mechanism regulating this type of congenital malformations is not yet clearly understood. While hereditary trait of some limb anomalies was proven in human and the responsible genetic impairments were found, this has not been confirmed in the other animals especially the birds. Regarding the different susceptibilities of various vertebrate species to the environmental and genetic factors in embryonic period, the probable cause of an embryonic defect in one species cannot be generalized to the all other species class. The present study reports a case of polymelia in an Iranian indigenous young fowl and discusses its possible causes. https://vrf.iranjournals.ir/article_24327_95d8f6ff68222377d570a652eb96f082.pdf