Faculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Evaluation of the relationship between inflammatory reaction and interleukins in ovine pulmonary adenocarcinomas1669793910.30466/vrf.2022.542311.3279ENEminKarakurtDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0003-2019-3690NüvitCoşkunDepartment of Virology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0001-7642-6460EnverBeytutDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0003-3360-2940Ömer FarukKeleşDepartment of Pathology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye0000-0002-7869-5311SerpilDağDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0001-7667-689XVolkanYilmazDepartment of Virology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0003-2752-5360HilmiNuhoğluDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0003-2530-2542AyferYıldızDepartment of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye0000-0002-6569-5435EmreKurtbaşHealth Sciences Institute, Kafkas University, Kars, Türkiye0000-0002-9752-194XJournal Article20211106In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 - 2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 – 2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor-associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.https://vrf.iranjournals.ir/article_697939_09d5b483deeae582b7727d15abef060a.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Rapid and visual detection of an isolated and identified goose parvovirus (GPV) strain by a loop-mediated isothermal amplification assay71269793810.30466/vrf.2021.540351.3246ENJun-TingLiuDepartment of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, ChinaYan-HuiChenDepartment of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, ChinaYi-FengPeiDepartment of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, ChinaQianYuDepartment of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, ChinaRuthAfumbaDepartment of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, ChinaHaoDongDepartment of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, ChinaEngineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, ChinaJournal Article20211005Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May 2020 and applied it to the loop-mediated isothermal amplification (LAMP) assay. We designed five sets of primers for the goose parvovirus VP3 gene by LAMP. The GV-1 primer set was selected to detect GPV sensitively and rapidly. LAMP was more sensitive compared to PCR. In addition, the LAMP method could complete detection within 60 min which was faster than the PCR assay. The LAMP provided a convenient and effective experimental method for detection of GPV for inspection and quarantine departments and health care units in China, and it is expected to become a simple and routine detection method, especially suitable for goose farms.https://vrf.iranjournals.ir/article_697938_b4d519abe867174b1dabb1b16ce7f655.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Evaluation of porcine circovirus type 2 double vaccination in weaning piglets that reared for gilts under field conditions131969793710.30466/vrf.2022.543079.3292ENVasileiosPapatsirosClinic of Medicine, Faculty of Veterinary Medicine, School of Health Sciences, University of Thessaly, Karditsa, GreeceGeorgiosPapakonstantinouClinic of Medicine, Faculty of Veterinary Medicine, School of Health Sciences, University of Thessaly, Karditsa, GreeceEleftheriosMeletisFaculty of Public and Integrated (One) Health, School of Health Sciences, University of Thessaly, Karditsa, GreeceDimitraBitchavaVet in Progress Plus, Veterinary LaboratoriesPolychronisKostoulasFaculty of Public and Integrated (One) Health, School of Health Sciences, University of Thessaly, Karditsa, GreeceJournal Article20211118The objective of the present study was to evaluate the efficacy of a porcine circovirus type 2 (PCV2) double vaccination in weaning piglets reared for gilts under field conditions. The study was conducted at a Greek farrow-to-finish conventional pig farm with a previous history of PCV2 infections. The trial included 96 female piglets at 21 days of age, which were equally allocated to two different study groups. Piglets of the group-1 received a single PCV2 vaccination at 21 days of age, while piglets of the group-2 were double vaccinated against PCV2, at 21 and 42 days of age. The results indicated that the piglets of group-2 had better growth performance, as they showed higher body weight (BW) and average daily weight gain (ADWG). In addition, ELISA tests showed that the double- vaccinated piglets presented a better humoral response against PCV2, as higher levels of IgG antibodies were detected in them than the piglets of the group-1. In conclusion, the current results suggested that a double PCV2 vaccination of piglets, reared for gilts, on a PCV2-affected farm could lead to higher protection against the virus.https://vrf.iranjournals.ir/article_697937_f9792f8dc7cffac2a798ff8421ce365e.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Identification of Salmonella carriers by amplification of FimA, Stn and InvA genes and bacterial culture methods in fecal samples of buffalo212869983710.30466/vrf.2022.544308.3312ENAbdulghaffarOwnaghDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran0000-0002-0119-2310NavidEtemadiDVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, IranPeymanKhademiDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran0000-0001-9393-9161HosseinTajikDepartment of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, IranJournal Article20211205Salmonellosis is one of the most important bacterial diseases in human and animals. Rapid diagnosis and sub sequence accurate treatment of <em>Salmonella</em> carriers help reduce the salmonellosis in human and livestock animals. In this study, 420 fecal samples were taken during year 2019 from buffalo in the Urmia, Khoy and Piranshahr regions in west Azerbaijan province, Iran. Samplings were carried out in different seasons. Presence of <em>Salmonella</em> invasion genes (<em>FimA</em>, <em>Stn</em> and <em>InvA</em>) were evaluated by polymerase chain reaction. The bacterial culture and biochemical tests were performed on feces samples for isolation of bacterium <em>Salmonella</em>; however, all samples were negative in culture method. PCR findings showed that, 50 (11.90%) fecal samples were positive to the genes. The analysis of results showed that frequency of salmonellosis outbreak in different parts of west Azerbaijan province followed a similar pattern and the incidence of salmonellosis according to forecast in the warm seasons (spring and summer) was more than in cold seasons (autumn and winter). The prevalence of <em>Salmonella</em> in buffalo’s feces based on warm and cold seasons were 32 (64.00%) and 18 (36.00%), respectively. The results showed significant difference between cold and warm season in the prevalence of salmonellosis. Therefore, the application of molecular technics is essential for the prevention and treatment of salmonellosis. The results also showed that specificity of PCR method was better than culture method for detection of <em>Salmonella</em> in feces sample.https://vrf.iranjournals.ir/article_699837_2560e9153fbf60fddffa903753a59705.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Apoptotic activity of Newcastle disease virus in comparison with nisin A in MDA-MB-231 cell line293769794210.30466/vrf.2022.542258.3297ENGholam AbbasGhorbankhaniDepartment of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IranAliMohammadiDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran0000-0002-5651-3329NasrinKazemipourDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranSolmazMorovatiDepartment of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran0000-0002-6269-1228BehrouzGharesi FardDepartment of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran0000-0002-0162-3395SaeedNazifi HabibabadiDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranMahdiHashempour SadeghianDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranJournal Article20211122Given the development of drug-resistant cancer cells, designing alternative approaches for cancer treatment seems essential. In this study, we evaluated the anti-tumor effects of nisin A and newcastle disease virus (NDV) on triple-negative MDA-MB-231 cell line. The MDA-MB-231 cell line was separately and in combination subjected to the different concentrations of a Vero-adapted NDV (JF820294.1) and nisin A. The oncolytic effects of these treatments were analyzed by different cytotoxic and apoptosis techniques including trypan blue staining, MTT assay, acridine orange (EB/AO) staining, colony assay and flow cytometry over time. Nisin A at doses of more than 20.00 μg mL<sup>-1</sup> could represent the anti-viral effects and interfere with the oncolytic activity of NDV. Moreover, the analyses indicated that the anti-proliferative and cytotoxic features of combination therapy were stronger than those of individual NDV groups. However, the most apoptotic effect was seen in NDV experimental groups. Taken together, the results from cytotoxicity tests, flow cytometry and colony assay showed that either of the oncolytic agents had significant effects at low concentrations 72 hr post-treatment. Thereby, they had the potential to be used as new approaches in cancer treatment.https://vrf.iranjournals.ir/article_697942_827ab3bd73a7a5aa8c5c0fb0aec49d7d.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Sergentomyia species identification and their screening for possible infection to Leishmania spp. in Kaleybar, East-Azerbaijan province, Iran394369983810.30466/vrf.2022.544929.3325ENFahimehFirouzjaieDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranVahidehMoin-VaziriDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranAlireza ZahraeiRamezaniDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranHamedBehniafarDepartment of Medical Parasitology, Sarab Faculty of Medical Sciences, Sarab, IranMehdiBadakhshanDepartment of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences, Urmia,
IranAdelSpotinImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, IranZabihZareiDepartment of Medical Parasitology and Mycology, School of Public
Health, Tehran University of Medical Sciences, Tehran, Iran.0000-0001-6762-5994Journal Article20211215Leishmaniasis is a protozoal and vector-borne disease. World health organization has considered the disease as a neglected tropical disease. <em>Phlebotomus</em> and <em>Lutzumyia</em> species (order: Diptera, family: Psychodidae) are human leishmaniasis vectors in new and old worlds. <em>Sergentomyia</em> spp. (Diptera, Psychodidae) are proven vectors of lizard leishmaniasis. Although some studies have identified human <em>Leishmania</em> parasites in <em>Sergentomyia</em>, their role in parasite circulation is unknown yet. Hence, the parasitological and molecular methods were used to study the possible <em>Leishmania</em> infection of <em>Sergentomyia</em> spp., in the human and canine visceral leishmaniasis endemic area in North West of Iran. Even though <em>Sergentomyia</em> specimens were caught in a dominant number compared to <em>Phlebotomus</em> spp., no <em>Leishmania</em> promastigote or DNA was detected in live-caught or sticky trap-caught specimens, respectively. <em>Sergentomyia</em> spp. are proven vectors of sauroleishmaniasis, and despite several global reports of <em>Leishmania</em> infection in <em>Sergentomyia</em> spp., such findings should be carefully interpreted to avoid false vector incriminations.https://vrf.iranjournals.ir/article_699838_38675152b289a3a1c4b3ab617ae8d474.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Association between uterine toxicity induced by chlorpyrifos and downregulation of heparin-binding epidermal growth factor and L-selectin genes455269796210.30466/vrf.2021.532844.3203ENParisaGheibiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran0000-0002-0645-317XZohreEftekhariBiotechnology Department, Pasteur Institute of Iran, Tehran, Iran0000-0001-9705-5179DelaramDoroudDepartment of Quality Control, Research and Production Complex, Pasteur Institute of Iran, Karaj, Iran0000-0003-4167-9921KazemParivarDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran0000-0002-2836-9710Journal Article20210704Various factors are effective in reducing the fertility rate. This experiment aimed to investigate chlorpyrifos (CPF), an organophosphate, that could alter the structure of the uterus and the molecules involved in parental and fetal. CPF was injected intraperitoneally in thirty mice for five days in a week (six weeks). The animals were euthanized on the 5<sup>th</sup> day of gestation, then their blood and uterus were collected for biochemical and histopathological assays. Exposure to CPF resulted in a significant reduction in maternal weight gain and the number of litters. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were significantly increased in blood serum of the CPF group compared with the control. The number of uterus glands, endometrium thickness, and the uterine cavity were changed following CPF injection. Additional investigation indicated that the expressions of <em>L-selectin, L-selectin ligand</em>, and <em>heparin-binding epidermal growth factor (HB-EGF)</em> as initial adhesion of mice blastocysts and maternal endometrium biomarkers were downregulated in the CPF group. Nevertheless, any mortality and abnormal clinical symptoms were not observed in the treated mice. This study revealed a potential molecular mechanism of continuous CPF-induced toxicity in fetal-maternal attachment without clinical symptoms.https://vrf.iranjournals.ir/article_697962_e1f406a40137f98fbb96919f3edb0b2c.pdfFaculty of Veterinary Medicine, Urmia UniversityVeterinary Research Forum2008-814014120230101Effects of double intravenous furosemide administration at different doses on clinical variables, electrocardiographic indices and serum electrolytes in miniature donkeys535770003610.30466/vrf.2020.127810.2954ENSeyed HesamaddinBanihashemiDepartment of Surgery, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranAmir SaeedSamimiDepartment of Clinical Sciences,
Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran0000-0003-4568-6619KamyabSamimiPars Hospital, Shiraz, IranJavadTajikDepartment of Clinical Sciences,
Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranJournal Article20200615The intravenous (IV) usage of diuretics such as furosemide may cause changes in clinical variables, electrocardiographic (ECG) indices and some serum electrolytes concentrations in miniature donkeys. The purpose of this study was to evaluate the effects of double intravenous (IV) administration of furosemide at different doses on the clinical variables, ECG indices and serum electrolytes levels in miniature donkeys. A total of 21 clinically and para-clinically healthy miniature donkeys with a mean age of 6.00 ± 1.00 years and weight of 95.00 ± 5.00 kg were used. The animals were randomly divided into three groups of control (normal saline) and low- (1.00 mg kg<sup>-1</sup>) and high-dose (2.00 mg kg<sup>-1</sup>) of furosemide. Two IV injections with 12-hr interval were administered in each animal. The clinical and ECG parameters were measured at 0 (baseline), 2 (T2), 24 (T24) and 48 (T48) hr after drug administration. Serum concentrations of electrolytes were measured at T0, T24 and T48. The results of this study showed no changes in clinical variables (heart rate, rectal temperature and respiratory rate) and ECG indices (amplitude and duration of P, QRS and T waves and duration of PR, QT and RR intervals) as well as no significant changes in serum electrolytes (sodium, potassium, chloride, phosphorus, calcium and magnesium) levels. Although no significant effect on clinical variables, electrolytes levels and ECG indices after double IV administration of furosemide (1.00 and 2.00 mg kg<sup>-1</sup>) in miniature donkeys was observed, absence of these effects does not mean that furosemide had no pharmacological effect.https://vrf.iranjournals.ir/article_700036_8b2d11c12fbcf59c749f295eaa5ccb77.pdf