Farrin Babaei-Balderlou; Samad Zare
Volume 3, Issue 3 , September 2012, , Pages 187-192
Abstract
The aim of the present study was to evaluate the effect of melatonin as an antioxidant on spatial navigation memory in male diabetic rats. Thirty-two male white Wistar rats weighing 200 ± 20 g were divided into four groups, randomly: control, melatonin, diabetic and melatonin-treated diabetic. ...
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The aim of the present study was to evaluate the effect of melatonin as an antioxidant on spatial navigation memory in male diabetic rats. Thirty-two male white Wistar rats weighing 200 ± 20 g were divided into four groups, randomly: control, melatonin, diabetic and melatonin-treated diabetic. Experimental diabetes was induced by intraperitoneal injection of 50 mg kg-1 streptozotocin. Melatonin was injected (10 mg kg-1 day-1, ip) for 2 weeks after 21 days of diabetes induction. At the end of administration period, the spatial navigation memory of rats was evaluated by cross-arm maze. In this study lipid peroxidation levels, glutathione-peroxidase and catalase activities were measured in hippocampus. Diabetes caused to significant decrease in alternation percent in the cross-arm maze, as a spatial memory index, compared to the control group (p < 0.05), whereas administration of melatonin prevented the spatial memory deficit in diabetic rats. Also melatonin injection significantly increased the spatial memory in intact animals compared to the control group (p < 0.05). Assessment of hippocampus homogenates indicated an increase in lipid peroxidation levels and a decrease in GSH-Px and CAT activities in the diabetic group compared to the control animals, while melatonin administration ameliorated these indices in diabetic rats. In conclusion, diabetes induction leads to debilitation of spatial navigation memory in rats, and the melatonin treatment improves the memory presumably through the reduction of oxidative stress in hippocampus of diabetic rats.
Ehsan Hosseini; Saeed Nafisi; Samad Zare
Volume 2, Issue 4 , December 2011, , Pages 222-225
Abstract
The main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the variations of corticosterone, Free-T3, Free-T4 and malonyl dialdehyde in plasma in 48 adult white New Zealand male rabbits. Animals were divided into six groups namely, C1 (normal diet, not ...
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The main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the variations of corticosterone, Free-T3, Free-T4 and malonyl dialdehyde in plasma in 48 adult white New Zealand male rabbits. Animals were divided into six groups namely, C1 (normal diet, not exposed), C2 (normal diet, sham exposed), T1 (normal diet, exposed to electromagnetic field), C3 (high-cholesterol diet, not exposed), C4 (high-cholesterol diet, sham exposed) and T2 (high-cholesterol diet, exposed to electromagnetic field). In eight separate experiments, sham exposed groups (C2 and C4), were exposed to sham stimulated (without electromagnetic stimulation) for 5 days, 2 hour/day and the rabbits of the treatment groups (T1 and T2) were treated with triangular form 10 Hz of electromagnetic field for 5 days, 2 hour/day, while the control groups (C1 and C3) had no any exposure. At the end of the exposure, after a 12- hour fasting period, blood samples were taken and level of corticosterone, Free-T3 and Free-T4 were measured by Elisa kits and level of malonyldialdehyde was measured by spectrophotometric method. The results indicated that the blood serum levels of Free-T3, Free-T4 and Corticosterone in the T1 and T2 groups were significantly increased compared to those of their own control groups (P < 0.05). Malonyldialdehyde levels in T2 animals showed a significant decrease compared to that of animals of C3 and C4 (P < 0.05). We conclude that 10 Hz pulsed electromagnetic field can alter the levels of Free-T3, Free-T4 and corticosterone in animals with both normal diet and hyperchlosterol diet and also alter the amount of malonyldialdehyde in animals with hyperchlosterol diet.
