Theriogenology
Sayed Mortaza Alavi Shoushtari; Siamak Asri Rezaie; Amir Khaki; Abulfazl Belbasi; Hamid Tahmasebian
Volume 6, Issue 3 , September 2015, , Pages 211-215
Abstract
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 ...
Read More
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 were pro-estrous, estrous, met-estrous and diestrous, respectively. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean (± SEM) total serum (77.10 ± 1.50 µg dL-1) and uterine fluid (296.40 ± 9.40 μg dL-1) Cu in cyclic cows was higher than the values of 54.00 ± 1.10 μg dL-1 and 133.40 ± 5.70 μg dL-1 in pre-pubertal calves, respectively. Blood serum (114.60 ± 3.20 μg dL-1) and the uterine fluid (349.90 ± 8.90 μg dL-1) Zn content in cyclic cows were also higher than those (98.80 ± 1.50 μg dL-1 and 246.6 ± 4.50 μg dL-1 respectively) in pre-pubertal calves. Serum Cu in pro-estrus and estrus were lower than those in other stages and also lower than those in the uterine fluid. The lowest serum Zn content was recorded in pro- and met-estrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle the uterine fluid Zn content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Cu and Zn were actively secreted in uterine lumen and were not dependent on blood serum. The values also increased after puberty.
Sayed Mortaza Alavi Shoushtari; Siamak Asri Rezaie; Amir Khaki; Abulfazle Belbasi; Hamid Tahmasebian
Volume 5, Issue 4 , December 2014, , Pages 301-305
Abstract
To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, ...
Read More
To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean ± SEM total serum and uterine fluid Ca in cyclic buffaloes were 8.68 ± 0.28 mg dL-1 and 8.10 ± 0.2 mg dL-1 vs. 6.76 ± 0.65 mg dL-1 and 7.90 ± 0.15 mg dL-1 in pre-pubertal calves, respectively. Blood serum Mg was not different in cyclic and pre-pubertal animals but the uterine fluid Mg in cyclic cows was higher than those in pre-pubertal calves. Serum Ca in pro-estrus and estrus were higher than those in other stages and also higher than those in the uterine fluid. The lowest Mg content of serum was recorded in diestrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle except for estrus the uterine fluid Mg content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Ca was passively secreted in uterine lumen and mostly dependent on blood serum Ca concentrations but Mg was secreted independently. The values (except for serum total Mg) also increased after puberty.
Shahram Nozad; Ali-Gholi Ramin; Siamak Asri Rezaie
Volume 3, Issue 4 , December 2012, , Pages 281-285
Abstract
Milk samples from high and low milk producer Holstein cows, were obtained during the morning and afternoon milking over a one week period. Overall, 1064 samples were tested within 14 times sampling in Urmia, Iran. Milk macro-mineral concentrations in the morning milking and in low producers were greater ...
Read More
Milk samples from high and low milk producer Holstein cows, were obtained during the morning and afternoon milking over a one week period. Overall, 1064 samples were tested within 14 times sampling in Urmia, Iran. Milk macro-mineral concentrations in the morning milking and in low producers were greater than in the afternoon and in high producers. The highest and lowest concentrations were observed in Na+ and Mg++, respectively. Mean milk values between low and high producers in the morning, afternoon and daily milking times were different (p < 0.05). The individual comparison of milk parameters between both groups in the different milking times were also different (p < 0.05). The results of correlation among macro-minerals in the morning, afternoon and overall milking showed significant and positive correlations among all macro-minerals except for Na+ and K+, in which there was a significant negative correlation (p < 0.05). The highest and lowest correlations were found between Ca++ and inorganic phosphorus (IP) (r=0.37, p < 0.05) and Na+ and IP (r=0.10, p < 0.05), respectively. It is concluded that the concentration of macro-minerals in different producers varied between milking times. The sodium concentration was the highest while Mg++ was the lowest among macro-minerals. The correlation between Ca++/ IP was the highest, while Na+/K+ revealed a negative correlation. Therefore, by organizing the appropriate macro-minerals in the ration, it would be possible to achieve an optimal purpose from animal husbandry.
Sayed Mortaza Alavi-Shoushtari; Siamak Asri Rezaie; Mozhgan Pak; Sajad Alizadeh; Roya Abedizadeh; Amir Khaki
Volume 3, Issue 3 , September 2012, , Pages 199-203
Abstract
To investigate uterine and serum copper (Cu) and zinc (Zn) concentrations variation during the bovine estrus cycle , 232 blood and genital tract samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by the examination of the ovaries and the uterine tonicity. ...
Read More
To investigate uterine and serum copper (Cu) and zinc (Zn) concentrations variation during the bovine estrus cycle , 232 blood and genital tract samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by the examination of the ovaries and the uterine tonicity. Of the 46 samples selected for use in the study, 13 were pro-estrus, 10 estrus, 8 metestrus, and 15 diestrus. The uterus was incised and uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. The total mean (± SEM) Cu concentrations in serum and uterine fluid samples, determined by spectrophotometry, were 66.1± 6.5 and 171.3 ± 33.2μg dL-1 respectively, which were significantly different, while total mean serum and uterine fluid Zn concentrations were 91.9 ± 5.4 and 291.6 ± 23.4 μg dL-1, which also showed a significant difference. The mean serum Cu values in different phases of the estrous cycle were not significantly different, while uterine fluid Cu content in pro-estrus and diestrus were significantly higher than those in estrus and metestrus, and were also significantly higher than those of the serum samples. The mean Zn value of serum samples at different stages of the cycle was not significantly different. The mean Zn value of the uterine fluid samples was also not significantly different in different stages, but in pro-estrus, metestrus, and in diestrus they were highly significantly different from those of the serum. These results showed that Cu concentrations in the uterine fluid vary at different stages of the cycle and are higher than those in the blood serum, but, the uterine Zn content does not vary during the estrous cycle and is much higher than those in the serum, that seems to be due to the secretory action of the uterine mucosa.
