Surgery
Shiva Amanollahi; Ahmad Reza Bahrami; Azadeh Haghighitalab; Haniyeh Shaterzadeh Yazdi; Hossein Kazemi Mehrjerdi
Volume 15, Issue 1 , January 2024, , Pages 27-34
Abstract
Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery ...
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Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery rate and the efficiency of treatments following SCI. In this study, the effect of extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the human indoleamine 2,3-dioxygenase-1 (IDO1) gene on serum albumin and glucose levels was investigated. After pre-clearing steps of 72-hr conditioned media, small EVs (sEVs) were isolated based on the ultra-filtration method. They were encapsulated with a chitosan-based hydrogel. Five experimental groups (female rats, N = 30, ~ 230 g) were considered, including SCI, sham, hydrogel, control green fluorescent protein (GFP)-EVs and IDO1-EVs. The 60.00 µL of hydrogel or hydrogels containing 100 µg sEVs from GFP or IDO1-EVs were locally injected immediately after SCI (laminectomy of the T10 vertebra and clip compression). After 8 weeks, non-fasting serum glucose and albumin levels were measured. The results indicated that the level of serum albumin in the animals received IDO1-EVs (3.52 ± 0.04) was increased in comparison with the SCI group (3.00 ± 0.94). Also, these animals indicated higher glucose levels in their serum (250.17 ± 69.61) in comparison with SCI ones (214 ± 45.34). Although these changes were not statistically significant, they could be considered as evidence for the beneficial effects of IDO1-EVs administration in the context of SCI to reduce hypoalbuminemia and improve energy consumption. More detailed experiments are required to confirm these results.
Clinical Pathology
Abdolrasoul Namjou; Esfandiar Heidarian; Mahmoud Rafieian-kopaei
Volume 9, Issue 4 , December 2018, , Pages 349-355
Abstract
In this study, the effects of Urtica dioica hydro-alcoholic extract were investigated on the blood glucose and lipid profiles of female ovariectomized and non-ovariectomized rats. In total, 32 adult female rats were divided into four groups (eight each) including control and ovariectomy groups as well ...
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In this study, the effects of Urtica dioica hydro-alcoholic extract were investigated on the blood glucose and lipid profiles of female ovariectomized and non-ovariectomized rats. In total, 32 adult female rats were divided into four groups (eight each) including control and ovariectomy groups as well as non-ovariectomy and ovariectomy groups treated with 200 mg kg-1 of Urtica dioica extract orally in the last five weeks of the study starting from the week 56th. The duration of the study was 60 weeks. Glucose, serum lipid profiles and pancreatic pathological alterations were determined in these groups at the end of experiment. Serum glucose, triglyceride (TG), very-low-density lipoprotein (VLDL), and TG/high-density lipoprotein (HDL) ratio indicated a significant increase in the healthy female rats under treatment with Urtica dioica extract compared to others. The TG, cholesterol, HDL, low-density lipoprotein (LDL) and VLDL showed a significant increase in menopaused rats compared to others. The interaction of consuming Urtica dioica extract and ovariectomy caused significant decreases in glucose, TG, VLDL, HDL/LDL ratio and TG/HDL ratio. Consumption of Urtica dioica extract by non-menopaused rats damaged the beta cells in Langerhans islets. Results of the present study revealed that the consumption of Urtica dioica extract is not beneficial and has diabetogenic effects in female non-ovariectomized rats compared to ovariectomized ones.
Rasoul Pirmohammadi; Ehsan Anassori; Zahra Zakeri; Mortaza Tahmouzi
Volume 5, Issue 3 , July 2014, , Pages 207-212
Abstract
To evaluate the effects of garlic on some blood metabolites in pre-partum dairy goats, the ration was supplemented with raw garlic at the doses of 0, 30, 50 and 70 g kg-1 of Dry matter (DM) in eight pregnant Mahabadi breed goats (59 ± 1 kg initial live weight) in a replicated Latin square design ...
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To evaluate the effects of garlic on some blood metabolites in pre-partum dairy goats, the ration was supplemented with raw garlic at the doses of 0, 30, 50 and 70 g kg-1 of Dry matter (DM) in eight pregnant Mahabadi breed goats (59 ± 1 kg initial live weight) in a replicated Latin square design during the last two months of pregnancy. Each experimental period lasted 14 days with the first 12 days used for diet adaptation and two days of data collection with a 3-days wash-out period between treatments. The results revealed a beneficial increasing effect of garlic (at the level of 70 g kg-1 of DM) on serum glucose concentration (p < 0.05). No effects of garlic supplementation on blood non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), total triglycerides, total protein, and albumin concentration were observed, except for serum cholesterol concentration, which was reduced (p < 0.05) with 70 g kg-1 of DM of garlic supplementation. Dry matter intake was the same between the different treatment groups and throughout the trial period. Concerning the blood indicators of negative energy balance, no significant effects were found for NEFA and BHB in pre-partum goats however, serum glucose was improved significantly, which showed that garlic supplementation may improve the efficiency of feed utilization. In conclusion, garlic, as feed additives in ruminant nutrition, holds promise for improving feed efficiency and controlling the negative energy balance.
