Mojtaba Karimipour; Gholam Hossein Farjah; Morteza Hassanzadeh; Masoumeh Zirak Javanmard
Volume 13, Issue 2 , June 2022, , Pages 233-239
Abstract
Skin flap necrosis has been remained as an unsolved problem in plastic and reconstructive surgeries. Here, we explored the effects of metformin post-treatment on random skin flap survival in rats. An 8.00 × 2.00 cm dorsal skin flap was created in 24 rats and they were then divided into three groups ...
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Skin flap necrosis has been remained as an unsolved problem in plastic and reconstructive surgeries. Here, we explored the effects of metformin post-treatment on random skin flap survival in rats. An 8.00 × 2.00 cm dorsal skin flap was created in 24 rats and they were then divided into three groups (n = 8) including Control, metformin (Met) 50.00 mg kg-1 and Met 100 mg kg-1. All animals were administrated orally until seven days after flap surgery. Flap survival, the number of blood vessels and mast cells in the flap tissues were analyzed. Vascular endothelial growth factor (VEGF) expression levels in flap tissues was also determined using immunohistochemical methods. The percentage of survival area in Met 50.00 mg kg-1 and Met 100 mg kg-1 groups were significantly higher compared to control. The blood vessel density and the VEGF positive cells in the viable areas of flaps showed a significant increase in Met 50.00 mg kg-1 group compared to control group. The results of this study suggested that treatment with metformin, especially with low dose following skin flap surgery was effective in improving the flap survival and increasing the neovascularization in the flaps tissues of rats.
Fish & Aquatic
Seyed Hossein Hoseinifar; Parviz Zare; Hamed Kolangi Miandare
Volume 6, Issue 4 , December 2015, , Pages 331-335
Abstract
The present study investigates the effects of different routes of inulin administration as prebiotic on gut microbiota and survival rate of Indian white shrimp post-larvae. Four hundred and fifty Indian white shrimp post-larvae (PL1) were stocked in nine tanks. The tanks were assigned into three treatments: ...
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The present study investigates the effects of different routes of inulin administration as prebiotic on gut microbiota and survival rate of Indian white shrimp post-larvae. Four hundred and fifty Indian white shrimp post-larvae (PL1) were stocked in nine tanks. The tanks were assigned into three treatments: feeding with inulin-treated (110 mg L-1) Artemia nauplii (I-T), feeding with inulin-enriched (110 mg L-1) Artemia nauplii (I-E) and control which repeated triplicates. Feeding trial was performed until PL11 stage and then gut microbiota was studied using culture based method. Also, survival rate was calculated at the end of feeding trial. Our results showed that feeding on inulin enriched or treated Artemia nauplii had no significant effect on total viable culturable autochthonous bacteria and Vibrio spp. levels of the gut microbiota (p > 0.05). However, a remarkable increase of lactic acid bacteria levels (LAB) was observed in I-E treatment (p < 0.05). Administration of inulin enriched Artemia nauplii significantly elevated survival rates of Indian white shrimp post-larvae (p < 0.05). These results encourage administration of prebiotic-enriched Artemia nauplii in post larval stage of Indian white shrimp but determination the mode of action of prebiotic on various aspects of shrimp larviculture merit further research.
