Kazi Faizul Azim; Saneya Risa Somana; Md Kamrul Hasan; Md Javed Foysal; Md Hazrat Ali; Tanjia Afrin Chowdhury; Md Nazmul Hossain
Volume 12, Issue 4 , December 2021, , Pages 505-510
Abstract
Escherichia coli associated infections are major threats in poultry industry owing to severe economic losses each year. This study was conducted to identify E. coli isolates, to evaluate their antibiotic sensitivity and to find out their virulence patterns from infected broilers of Sylhet city in Bangladesh. ...
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Escherichia coli associated infections are major threats in poultry industry owing to severe economic losses each year. This study was conducted to identify E. coli isolates, to evaluate their antibiotic sensitivity and to find out their virulence patterns from infected broilers of Sylhet city in Bangladesh. Using polymerase chain reaction, a total 20 isolates were identified as E. coli from 11 chickens, exhibiting symptoms like colibacillosis and/or diarrhea. All isolates were positive for type-1 fimbrial adhesion (fimH), followed by putative avian hemolysin (hlyF) in 17 isolates; while none of the isolates was amplified with intimin (eaeA). Among 10 tested antibiotics, 100% of the isolates (n = 20) showed resistance to ampicillin, amoxicillin and tetra-cycline; but they were 100% sensitive to gentamicin. Organ specific correlations of antibiotic sensitivity were obtained among the isolates through principal component analysis (PCA) and Agglomerative Hierarchical Clustering (AHC). The 16S rRNA data of two multi-drug resistant isolates revealed closed clustering with clinical E. coli strains which could be indication of their zoonotic potential. In conclusion, the results depict higher prevalence of fimH and hlyF genes and drug resistance patterns of E. coli isolates from broilers in Sylhet city of Bangladesh.
Poultry
Saeed Rasoulinezhad; Mohammad Hassan Bozorgmehrifard; Hossein Hosseini; Nariman Sheikhi; Saeed Charkhkar
Volume 8, Issue 4 , December 2017, , Pages 293-298
Abstract
Mycoplasma gallisepticum (MG) is economically important pathogen of poultry causes airsacculitis and frequently infraorbital sinusitis in turkeys. Infections may remain without clinical signs, but they can make birds susceptible to secondary infections.This study was carried out for molecular detection ...
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Mycoplasma gallisepticum (MG) is economically important pathogen of poultry causes airsacculitis and frequently infraorbital sinusitis in turkeys. Infections may remain without clinical signs, but they can make birds susceptible to secondary infections.This study was carried out for molecular detection and phylogenetic analysis of MG infections in commercial and backyard turkey flocks in some parts of Iran. A total number of 600 swab samples were collected from 18 commercial and 31 backyard turkey flocks. The PCR technique was performed for detecting 16S rRNA gene in the samples. Positive sample were subjected for sequencing of mgc2 gene. The results showed that 48.38% of backyard and 16.66% of commercial farms were positive for MG. These findings suggested the presence of MG in the commercial and backyard turkeys’ farms of Iran. The molecular analysis indicated high sequence similarity between some Iranian turkeys isolates with Indian and Pakistanian MG isolates. Furthermore, substitutions of MG nucleic acids and correlated amino acids sequences may lead to some antigenic modifications.
