Theriogenology
Nasim Ahmad; Muhammad Awais Ajmal; Mubbashar Hassan; Muhammad Shahzad; Muhammad Ilyas Naveed; Aijaz Ali Channa
Articles in Press, Accepted Manuscript, Available Online from 30 April 2024
Abstract
The Cholistani cow is considered one of the milch breeds of Zebu cattle. Despite being heat and tick resistant, it has not yet received much popularity due to its long calving interval among Pakistan dairy farmers. This study aimed to know the basic reproductive physiology of Cholistani cows using sonography ...
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The Cholistani cow is considered one of the milch breeds of Zebu cattle. Despite being heat and tick resistant, it has not yet received much popularity due to its long calving interval among Pakistan dairy farmers. This study aimed to know the basic reproductive physiology of Cholistani cows using sonography and reproductive biotechnologies such as estrus synchronization and timed artificial insemination to improve reproductive efficiency. In Experiment 1, six Cholistani cows with mixed parity 3.2 ± 1.3 and 400 kg weight were selected to monitor ovarian dynamics on alternate days by the same sonographer from the onset of heat followed by ovulation till the next ovulation. Experiment 2 measured the effect of estrus synchronization methods, CIDR-GnRH (n=31) and Ovsynch (OVS, n=32) on various reproductive parameters. The mean estrous cycle length was 19.8 ± 0.5 days with two follicular waves. The mean inter-ovulatory interval was 20.8 ± 0.5 days, having a preovulatory follicular size of 13.8 ± 2.3 mm. Estrus response was higher (P > 0.05) in CIDR-GnRH (94%) than in OVS (84%) cows. Similarly, ovulation and conception rates were higher (P > 0.05) in CIDR-GnRH (92% vs. 68%) than in OVS cows (41% vs. 33%), respectively. It is concluded that using CIDR-GnRH results in a better estrus response, higher ovulation rate, and subsequently greater conception rate than OVS in Bos indicus dairy cows.
Theriogenology
Mohammad Bolghanabadi; Hamideh Salari Sedigh; Pezhman Mirshokraei; Masoud Rajabioun
Volume 14, Issue 12 , December 2023, , Pages 665-671
Abstract
This study designed a protocol that would combine pregnant mare serum gonadotrophin (PMSG) and cabergoline (CAB) to induce estrus in bitches. Twenty clinically healthy adult and anestrous female dogs were randomly assigned into four groups. The first group was treated with 5.00 μg kg-1 CAB until the ...
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This study designed a protocol that would combine pregnant mare serum gonadotrophin (PMSG) and cabergoline (CAB) to induce estrus in bitches. Twenty clinically healthy adult and anestrous female dogs were randomly assigned into four groups. The first group was treated with 5.00 μg kg-1 CAB until the onset of proestrus or for 25 days. The second group was treated with 20.00 IU kg-1 PMSG for 5 days and 500 IU human chorionic gonadotropin (hCG) on the 5th day. The third group was treated with 5.00 μg kg-1 CAB for 10 days in combination with 20.00 IU kg-1 PMSG for 5 days and 500 IU hCG on the 10th day. The control group received 1.00 mL of normal saline. Ovarian changes were evaluated ultrasonographically, and the estrus cycle phase was examined by vaginal cytology. Respectively, three, three and four bitches showed clinical signs of proestrus in each treatment group. The intervals between treatment and proestrus for each group were 30.00 ± 3.05, 7.67 ± 1.20 and 13.00 ± 1.20 days, respectively. Two weeks after estrus, the progesterone mean was 14.51 ± 6.24, 19.96 ± 17.16 and 19.12 ± 9.26 ng mL-1 for each group, respectively. In ultrasonography examination, the largest follicle was identified at 15.66 ± 1.33, 11.66 ± 2.40 and 8.75 ± 2.17 days after the onset of proestrus and the largest follicle’s size was measured 6.50 ± 0.55, 4.83 ± 1.64 and 7.07 ± 1.49 mm for each group, respectively. Although the combined use of CAB and PMSG reduced the duration of treatment, alteration of the duration or PMSG dosage can be helpful to improve the results.
