Veterinary Research Forum
Vet Res Forum

Molecular Detection of Anaplasma bovis in Cattle from Central Part of Iran

Document Type : Original Article

Authors

Vahid Noaman 1
Parviz Shayan 2

1 Department of Veterinary Research, Isfahan Research Center for Agriculture and Natural Resources, Isfahan, Iran

2 Center for Ticks and Tick-borne Diseases, Faculty of Veterinary Medicine, University of Tehran and Investigating Group Molecular Biological System Transfer, Tehran, Iran

Abstract
Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran. The presence of A. bovis examined using light microscopic detection and species-specific nested polymerase chain reaction (nested-PCR) based on 16S rRNA gene. Of the 150 cattle, 4 (2.66 %) was positive for A. bovis by nested-PCR. These data is the first A. bovis DNA presence in cattle from central part of Iran.

Keywords

A. bovis
Nested-PCR
16s rRNA gene
Iran
Veterinary Research Forum
Volume 1, Issue 2 - Serial Number 2
September 2010
Pages 117-122

  • Receive Date 25 December 2012

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