Potential fertilizer of spermatozoa in porcine epididymal tail post-orchiectomy.

Document Type : Original Article

Authors

1 Doctorado en Ciencias Biológicas y de la Salud. Universidad Autónoma Metropolitana Xochimilco

2 3Centro de Enseñanza, Investigación y Extensión en Producción Porcina (CEIEPP). Facultad de Medicina Veterinaria y Zootecnia: Universidad Nacional Autónoma de México. Jilotepec, Mexico State. Mexico

3 UAM X DEPARTAMENTO DE PRODUCCION AGRICOLA Y ANIMAL

4 Department of Agricultural and Animal Production.

10.30466/vrf.2020.108342.2570

Abstract

The aim of this study was to determine the potential fertilizing of spermatozoa from the epididymal tail in different periods of time post-orchiectomy (P-OQ). Therefore, the study was approached in two stages. In the first stage, the orchiectomy was performed in 30 adult pigs; the testicles were stored at 5 °C in physiological saline solution for 5, 24, 48, 72, 96 and 120 hr. The spermatozoa were obtained by backflushing the vas deferens. The spermogram and fluorometric study were performed for each sample to evaluate the exposure of phosphatidylserine (PS) and acrosome reaction (AR). The second stage included the fertilization test, 16 prepubertal sows were selected, after synchronizing the oestrous cycle, the post-cervical artificial insemination was performed with the refrigerated sperm samples from each P-OQ time. The percentage of live sperm remained without significant changes until 96 hrs. P-OQ. An increase in the percentage of spermatozoa that showed a PS exposure was observed. The premature AR was evident after 72 hr. considering that the artificial insemination was performed ensuring a minimum number of live sperms;, no significant differences were observed in the number of embryos and corpora lutea. The results indicate that pig sperm collected from the epididymal tail P-OQ and stored for 5 and up to 72 hr. at 5 °C has viable characteristics and maintain their fertilization ability. However, there is an increase in the loss of phospholipid asymmetry of the plasma membrane as time increases (72 and 96 hr.), so that sperm viability decreases.

Keywords



Articles in Press, Accepted Manuscript
Available Online from 14 June 2021
  • Receive Date: 22 May 2019
  • Revise Date: 24 April 2020
  • Accept Date: 09 June 2020