Document Type : Original Article
Authors
- Mariela Adriana Ydiaquez-Miranda 1
- José Antonio Herrera-Barragán 2
- Miguel González-Lozano 2, 3
- Alejandro Ávalos-Rodríguez 2
1 PhD in Biological and Health Sciences, Metropolitan Autonomous University, Mexico City, Mexico
2 Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, Mexico City, Mexico
3 Center for Teaching, Research and Extension in Swine Production (CEIEPP), Faculty of Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Jilotepec, Mexico
Abstract
The aim of this study was to determine the potential fertilizing of spermatozoa from the epididymal tail in different periods of time post-orchiectomy (P-OQ). Therefore, the study was approached in two stages. In the first stage, the orchiectomy was performed in 30 adult pigs. The testicles were stored at 5.00 ˚C in physiological saline solution for 5, 24, 48, 72, 96 and 120 hr. The spermatozoa were obtained by backflushing the vas deferens. The spermogram and fluorometric study were performed for each sample to evaluate the exposure of phosphatidyl-serine (PS) and acrosome reaction (AR). The second stage included the fertilization test, 16 prepubertal sows were selected, after synchronizing the oestrous cycle and the post-cervical artificial insemination was performed with the refrigerated sperm samples from each P-OQ time. The percentage of live sperm remained without significant changes until 96 hr P-OQ. An increase in the percentage of spermatozoa that showed a PS exposure was observed. The premature AR was evident after 72 hr. Considering that the artificial insemination was performed ensuring a minimum number of live sperms, no significant differences were observed in the number of embryos and corpora lutea. The results indicated that pig sperm collected from the epididymal tail P-OQ and stored for 5 and up to 72 hr at 5.00 ˚C had viable characteristics and maintained their fertilization ability. However, there was an increase in the loss of phospholipid asymmetry of the plasma membrane as time increased (72 and 96 hr), therefore, sperm viability was decreased.
Keywords
- Bertol MAF, Weiss RR, Fujita AS, et al. Two commercial extenders for cryopreservation of epididymal bull sperm [Portuguese]. Ciência Rural 2014; 44(9): 1658-1663.
- Strand J, Ragborg, MM, Pedersen, HS, et al. Effects of post-mortem storage conditions of bovine epididymides on sperm characteristics: investigating a tool for preservation of sperm from endangered species. Conserv Physiol 2016; 4(1). doi: 10.1093/ conphys/cow069.
- Lima DBC, Silva TFP, Aquino Cortez A, et al. Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders. Arq Bras Med Vet Zootec 2016; 68(04): 873-881.
- Chen SU, Shieh JY, Wang YH, et al. Pregnancy achieved by intracytoplasmic sperm injection using cryo-preserved vasal-epididymal sperm from a man with spinal cord injury. Arch Phys Med Rehabil 1998; 79(2):218-221.
- Martins CF, Driessen K, Costa PM, et al. Recovery, Cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5 degrees C by different periods of time. Anim Reprod Sci 2009; 116(1-2):50-57.
- Nakatsukasa E, Inomata T, Ikeda T, et al. Generation of live rat offspring by intrauterine insemination with epididymal spermatozoa cryopreserved at -196 degrees C. Reproduction 2001; 122(3):463-467.
- Turri F, Madeddu M, Gliozzi TM, et al. Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve gene banking in local breeds. Animal 2014; 8(3):440-447.
- Martinez-Pastor F, Guerra C, Kaabi M, et al. Decay of sperm obtained from epididymes of wild ruminants depending on postmortem time. Theriogenology 2005; 63(1):24-40.
- Kunkitti P, Bergqvist AS, Sjunnesson Y, et al The ability of feline spermatozoa in different epididymal regions to undergo capacitation and acrosome reaction. Anim Reprod Sci 2015; 161:64-74.
- Tittarelli C, Savignone CA, Arnaudín MC, et al. Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epidi-dymides. Theriogenology 2006; 66(6-7):1637-1640.
- Alberts AMI, Barrios ADR. Individual mobility of postmortem bull epididymal sperm obtained by retrograde lavage [Spanish]. Zootec Trop 2006; 24(3):267-280.
- Saenz JR. Cryopreservation of white-tail deer epididymal sperm for artificial insemination. Master's Thesis. Louisiana State University and Agricultural and Mechanical College. Louisiana, USA: 2007. Available at: https://digitalcommons.lsu.edu/gradschool_theses/3362. Accessed July 5, 2021.
- Kozdrowski R, Niżański W, Dubiel A, et al. Possibilities of using the European bison (Bison bonasus) epididymal spermatozoa collected post-mortem for cryopreservation and artificial insemination: a pilot study. Reprod Biol Endocrinol 2011; 9:31. doi: 10.1186/1477-7827-9-31.
- Matás C, Sansegundo M, Ruiz S, et al. Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa. Theriogenology 2010; 74(8):1327-1340.
- Malo C, Gil L, Cano R, et al. Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology 2011; 75(9):1735-1741.
- Official Mexican Standard Nom-033-Zoo-1995, Humane Slaughter of Domestic and Wild Animals. United States of Mexico [Spanish]. Secretariat of Agriculture, Livestock and Rural Development. Roberto Zavala Echavarria, General Legal Director of the Ministry of Agriculture, Livestock and Rural Development, based on articles 35 section IV of the Organic Law of the Federal Public Administration; 4th Sections I, III and V, 12, 16, 17 and 44 of the Federal Law of Animal Health; 1, 38 section II, 40 sections III and XI, 41 and 47 section IV of the Federal Law on Metrology and Standardization; 12, sections XXIX and XXX of the Internal Regulations of this Unit.
- Ponglowhapan S, Chatdarong K. Effects of Equex STM Paste on the quality of frozen-thawed epididymal dog spermatozoa. Theriogenology 2008; 69(6): 666-672.
- Ochoa G, Acosta MJ, Rueda M, et al. Evaluation of pig semen preserved in long-term diluents [Spanish]. In vitro Rev Comput de Prod Porc 2008; 13: 239-245.
- Ávalos RA, González SJA, Vargas IAK, et al. In vitro seminal collection and manipulation manual [Spanish]. 1st Mexico: Metropolitan Autonomous University. 2018; 28-33.
- Bakst MR, Cecil HC. Nigrosin/eosin stain for determining live/ dead and abnormal sperm counts. In Bakst MR, Wishart GJ, Savoy IL (Eds). Techniques for semen evaluation, semen storage and fertility determination. USA: Cornell University, Poultry Science Association 1997; 29-35.
- Méndez Y, Báez F, Villamediana P. Effect of in vitro exposure of human sperm to cadmium (CdCl2) [Spanish]. Perinatol Reprod Hum 2011; 25(4):198-204.
- Knox RV. Artificial insemination in pigs today. Theriogenology 2016; 85(1):83-93.
- García-Vázquez FA, Matás C. In vitro embryo culture in swine species (I). Breeding and health. [Spanish]. Cría y salud 2010; 33: 40-45.
- Herrera-Barragán JA, Ávalos Rodríguez A, Zaldívar-Martínez et al. In vitro fertilization, production and management of bovine embryos [Spanish]. 1st Mexico: Metropolitan University 2013; 20-23.
- Kikuchi K, Nagai T, Kashiwazaki N, et al. Cryo-preservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4 degrees C. Theriogenology 1998; 50(4): 615-623.
- Okazaki T, Akiyoshi T, Kan M, et al. Artificial insemination with seminal plasma improves the repro-ductive performance of frozen-thawed boar epididymal spermatozoa. J Androl 2012; 33(5): 990-998.
- Umeyama K, Honda K, Matsunari H, et al. Production of diabetic offspring using cryopreserved epididymal sperm by in vitro fertilization and intrafallopian insemination techniques in transgenic pigs. J Reprod Dev 2013; 59(6):599-603.
- Avalos-Rodríguez A, Ortíz-Muñíz AR, Ortega-Camarillo C, et al. Fluorometric study of rabbit sperm head membrane phospholipid asymmetry during capacitation and acrosome reaction using Annexin-V FITC. Arch Androl 2004; 50(4): 273-285.
- Kotwicka M, Jendraszak M, Jendraszak P. Phosphatidylserine membrane translocation in human spermatozoa: topography in membrane domains and relation to cell vitality. J Membr Biol 2011; 240(3):165-170.
- Martin G, Sabido O, Durand P, et al. Phosphatidylserine externalization in human sperm induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction. Hum Reprod 2005; 20(12): 3459-3468.
- Gupta SK, Bhandari B. Acrosome reaction: relevance of zona pellucida glycoproteins. Asian J Androl 2011; 13(1): 97-105.
- de Vantéry Arrighi C, Lucas H, Chardonnens D, et al. Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential. Reprod Biol Endocrinol 2009; 7(1). doi:10.1186/1477-7827-7-1.
- Johnson LA, Weitze KF, Fiser P, et al. Storage of boar semen. Anim Reprod Sci 2000; 62(1-3):143-172.
- Schulze M, Henning H, Rüdiger K, et al. Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions. Theriogenology 2013; 80(9): 990-998.
- Hoseinzadeh-Sani SKA, Barati F, Khaksary Mahabady M. The effects of ex vivo cold-storage on cryopreservation of the goat (Caprus hircus) epididymal sperm. Iran J Reprod Med. 2013; 11(9):747-752.
- Oh SA, Park YJ, You YA, et al. Capacitation status of stored boar spermatozoa is related to litter size of sows. Anim Reprod Sci 2010; 121(1-2):131-138.
- Kotwicka M, Jendraszak M, Skibinska I, et al. Decreased motility of human spermatozoa presenting phosphatidylserine membrane translocation-cells selection with the swim-up technique. Hum Cell 2013; 26(1):28-34.
- Vazquez JM, Roca J, Gil MA, et al. New developments in low-dose insemination technology. Theriogenology 2008; 70(8):1216-1224.
- Broekhuijse MLWJ, Feitsma H, Gadella BM. Artificial insemination in pigs: predicting male fertility. Vet Q 2012; 32(3-4):151-157.
- Ringleb J, Waurich R, Wibbelt G, et al. Prolonged storage of epididymal spermatozoa does not affect their capacity to fertilise in vitro-matured domestic cat (Felis catus) oocytes when using ICSI. Reprod Fertil Dev 2011; 23(6):818-825.
- Bertol MAF, Weiss RR, Kozicki LE, et al. In vitro and in vivo fertilization potential of cryopreserved spermatozoa from bull epididymides stored for up to 30 hours at ambient temperature (18 °C - 20 °C). Theriogenology 2016; 86(4):1014-1021.
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