Ethephon causes reproductive malfunction in male mice: Histological and biochemical evidence

Document Type : Original Article


Urmia University



Ethephon (ETP) is an organophosphorus pesticide used in agriculture for early ripening of fruits and vegetables. The aim of this study was to investigate the effects of ETP on testicular histoarchitecture and biochemical parameters in mouse. The ETP was administered orally at different doses (120, 240 and 480 mg kg-1) daily for 35 days. Control, sham and neostigmine-treated (0.10 mg kg-1 daily; orally) groups were also considered. Following 35 days, animals were euthanized and testicle and serum samples were taken. In ETP-administered animals, a decrease in acetylcholine esterase, total antioxidant capacity and catalase levels and an increase in testicular tissue malondialdehyde concentration were observed. Reductions were also seen in the germinal epithelium height, interstitial tissue thickness, seminiferous tubules diameter and Leydig cell number as well as spermiogenesis and Sertoli cell indices in ETP-treated mice compared to control ones. Histological and biochemical findings in this study indicate that ETP can have repro-toxic effects in male mice.


Articles in Press, Accepted Manuscript
Available Online from 14 June 2021
  • Receive Date: 17 June 2019
  • Revise Date: 23 October 2019
  • Accept Date: 06 January 2020