Protective effect of cinnamic acid on orthophenylphenol-induced oxidative ‎stress in rats

Document Type : Original Article

Authors

1 Department of Chemistry, Faculty of Arts and Sciences, Burdur Mehmet Akif Ersoy University, Burdur, Turkiye

2 Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkiye

Abstract

This study aimed to evaluate the protective effect of cinnamic acid (CA) on orthophenyl-phenol (OPP)-induced oxidative stress in rats. Thirty-two Sprague-Dawley male rats were divided into four groups as control, OPP, CA and OPP + CA groups. The animals in control, OPP and CA groups were received corn oil, OPP (700 mg kg-1 dissolved in corn oil) and CA (200 mg kg-1) by gavage for 21 days, respectively. The animals in OPP + CA group were received CA for 3 days and from day 4; OPP and CA were applied together daily until day 25. Blood and liver samples were collected at the end of experiment for measurement of aminotransferases, creatinine (CREA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), reduced glutathione (GSH) and malondialdehyde (MDA). The OPP-induced high serum activities of aminotransferases and level of CREA were reduced significantly by CA administration. The OPP induced significant increases of CAT activities and MDA levels in serum and liver tissue samples. Moreover, OPP significantly decreased GSH levels as well as GSH-Px and SOD activities. Pre-treatment with CA ameliorated the GSH levels along with GSH-Px and SOD activities compared to OPP-receiving rats. On the other hand, CAT activities and MDA levels significantly decreased following the pre-treatment with CA compared to OPP-receiving rats. It has been determined that OPP causes oxidative stress and lipid peroxidation in blood and liver tissues and creates changes in anti-oxidant defense enzymes. Pre-treatment with CA reduces lipid peroxidation and provides protective effect against oxidative stress.

Keywords


  1. Balakrishan S, Eastmond DA. Micronuclei and cell proliferation as early biological markers of orho-phenylphenol-induced changes in the bladder of male F344 rats. Food Chem Toxicol 2006; 44(8): 1340-1347.
  2. 2-Phenylphenol in Drinking-water. Background document for development of WHO Guidelines for Drinking-water Quality, 2003. Available at: http://www.who.int/water_sanitation_health/dwq/chemicals/2phenylphenol.pdf. Accessed March 30, 2022.
  3. Appel KE. The carcinogenicity of the biocide ortho-phenylphenol. Arch Toxicol 2000; 74(2): 61-71.
  4. Kwok ESC, Silva M. Re-evaluation of developmental and reproductive toxicity of ortho-phenylphenol (OPP) and sodium ortho-phenylphenate. Cell Dev Biol 2013; 2(3): 1-12.
  5. Perruchon C, Patsioura V, Vasileiadis S, et al. Isolation and characterization of a Sphingomonas strain able to degrade the fungicide ortho-phenylphenol. Pest Manag Sci 2016; 72(1): 113-124.
  6. Grossman J. What's hiding under the sink: dangers of household pesticides. Environ Health Perspect 1995;103(6): 550-554.
  7. Nakagawa Y, Tayama K. Effect of buthionine sulfoximine on ortho-phenylphenol-induced hepato- and nephrotoxic potential in male rats. Arch Toxicol 1988; 62: 452-457.
  8. Guzman JD. Natural cinnamic acids, synthetic derivatives and hybrids with antimicrobial activity. Molecules 2014; 19(12): 19292-19349.
  9. Yan SL, Wang ZH, Yen HF, et al. Reversal of ethanol-induced hepatotoxicity by cinnamic and syringic acids in mice. Food Chem Toxicol 2016; 98(Pt B): 119-126.
  10. Alam MA, Subhan N, Hossain H, et al. Hydroxycinnamic acid derivatives: a potential class of natural compounds for the management of lipid metabolism and obesity. Nutr Metab (Lond) 2016;13: 27. doi:10.1186/s12986-016-0080-3.
  11. Song F, Li H, Sun J, et al. Protective effects of cinnamic acid and cinnamic aldehyde on isoproterenol-induced acute mycocardial ischemia in rats. J Ethnopharmacol 2013; 150(1): 125-130.
  12. Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. International Federation of Clinical Chemistry and Laboratory Medicine. Part 4. Reference procedure for the measurement of catalytic concentration of alanine aminotransferase. Clin Chem Lab Med 2002; 40(7): 718-724.
  13. Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. International Federation of Clinical Chemistry and Laboratory Medicine. Part 5. Reference procedure for the measurement of catalytic concentration of aspartate aminotransferase. Clin Chem Lab Med 2002; 40(7): 725-733.
  14. Jaffe M. The precipitation produced by picric acid in normal urine and a new reaction of creatinine. [German] Z Physiol Chem 1886; 10: 391-400.
  15. Aebi H. Catalase in vitro. Methods enzymol 1984; 105: 121-126.
  16. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab Clin Met 1967; 70(1): 158-169.
  17. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968; 25(1): 192-205.
  18. Yoshioka T, Kawada K, Shimada T, et al. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 1979; 135(3): 372-376.
  19. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-358.
  20. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem 1949; 177(2): 751-766.
  21. Bomhard EM, Brendler-Schwaab SY, Freyberger A, et al. O-phenylphenol and its sodium and potassium salts: a toxicological assessment. Crit Rev Toxicol 2002; 32(6): 551-625.
  22. Li J, Yang G, Wang S, et al. The protective effects of hydroxytyrosol against ortho-phenylphenol-induced DNA damage in HepG2 cells. Toxicol Mech Methods 2012; 22(6): 432-437.
  23. Santos PMP, Vieira AJSC. Antioxidising activity of cinnamic acid derivatives against oxidative stress induced by oxidising radicals. J Phys Org Chem 2013; 26(5): 432-439.
  24. Tohamy AA, Aref AM, Abdel Moneim AE, et al. Cinnamic acid attenuates cisplatin-induced hepatotoxicity and nephrotoxicity. J Bas Environ Sci 2016; 3: 1-9.
  25. El-Sayed el-SM, Abd El-Raouf OM, Fawzy HM, et al. Comparative study of the possible protective effects of cinnamic acid and cinnamaldehyde on cisplatin-induced nephrotoxicity in rats. J Biochem Mol Toxicol 2013; 27(12): 508-514.
  26. Patra K, Bose S, Sarkar S, et al. Amelioration of cyclophosphamide induced myelosupression and oxidative stress by cinnamic acid. Chem Biol Interact 2012; 195(3): 231-239.
  27. Abd El-Raouf OM, El-Sayed EM, Manie MF. Cinnamic acid and cinnamaldehyde ameliorate cisplatin-induced splenotoxicity in rats. J Biochem Mol Toxicol 2015; 29(9): 426-431.
  28. Murata M, Moriya K, Inoue S, et al. Oxidative damage to cellular and isolated DNA by metabolites of a fungicide ortho-phenylphenol. Carcinogenesis 1999; 20(5): 851-857.
Volume 13, Issue 2
June 2022
Pages 187-192
  • Receive Date: 13 March 2020
  • Revise Date: 01 June 2020
  • Accept Date: 20 June 2020
  • First Publish Date: 05 April 2022