Veterinary Research Forum
Vet Res Forum

Effects of Urtica dioica agglutinin lectin on glycotargeting of the vasculature: an in ovo study on chicken embryo

Document Type : Original Article

Authors

Esmaeil Samadian 1
Abasalt Hosseinzadeh Colagar 1
Jahanbakhsh Asadi 2

1 Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran

2 Metabolic Disorders Research Center, Golestan University of Medical Science, Gorgan, Iran

https://doi.org/10.30466/vrf.2020.136565.3057
Abstract
The angiogenesis process is a pivotal cellular process involved in both developmental and pathological circumstances. In this study we investigated effect of Urtica dioica agglutinin (UDA), as an unusual phyto-lectin from the chitin-binding protein family, on the angiogenesis of chicken embryos. The UDA was extracted from plant rhizomes and purified by affinity chromatography column. The activity of this lectin was assayed by hemagglutination test on the human RBCs. Anti-angiogenic effect of UDA on the extra-embryonic layer of the chick egg was studied in the different concentrations. Our results showed that the minimum concentration of UDA for agglutination were 48.00 and 15.00 µg mL-1 in macro- and microscopic studies, respectively. Because the number and length of the vessels were dramatically decreased at 100 µg kg-1 of UDA, the lectin had an inhibitory effect on angiogenesis of the embryonic vasculature of the chick. We concluded that UDA might target the vascularization events through binding to GlcNAc-conjugates. More investigations are needed to clarify the angiogenesis-related therapeutic roles of this interesting biomolecule.

Keywords

Angiogenesis
Animal model
Chicken embryo
Lectin activity
Urtica dioica agglutinin
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Veterinary Research Forum
Volume 13, Issue 3
Summer 2022
Pages 379-385

  • Receive Date 16 September 2020
  • Revise Date 31 October 2020
  • Accept Date 28 November 2020

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