Veterinary Research Forum
Vet Res Forum

An ethyl acetate fraction of flavonoids from Polygonum hydropiper L. exhibits an anti-inflammatory activity in PCV2-infected porcine alveolar macrophages via PI3K/Akt and NF-κB pathways

Document Type : Original Article

Authors

Chun-Zhi Ren 1, 2
Wen-Yue Hu 3
Man-ling Song 1
Ying-Yi Wei 1
Ting-Jun Hu 1

1 Department of Veterinary Pharmacology, College of Animal Science and Technology, Guangxi University, Nanning, China

2 Department of Animal Science and Technology, Guangxi Agricultural vocational and Technical University, Nanning, China

3 Department of Sciences and Biotechnology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

https://doi.org/10.30466/vrf.2021.137675.3070
Abstract
Porcine circovirus type 2 (PCV2) widely exists in swine production systems causing porcine circovirus diseases (PCVD) which is associated with significant economic losses. Polygonum hydropiper L. was used as a traditional Chinese medicine to treat a variety of diseases. This study was carried out to investigate anti-inflammatory activity of the ethyl acetate fraction of flavonoids from Polygonum hydropiper L. (FEA) in PCV2-induced porcine alveolar macrophages (3D4/2 cell line). The production of oxygen species (ROS) and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-8 (IL-8) were detected to evaluate the anti-inflammatory activities of FEA. The translocation of nuclear factor-kappa B (NF-κB) and the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) signaling pathways were investigated to document the potential anti-inflammatory mechanisms. In PCV2 induced 3D4/2 cells, FEA treatment significantly reduced the production of ROS, and sharply down-regulated the levels of TNF-α, IL-1β and IL-8 in both secretion and mRNA expression level. The FEA also decreased the mRNA expression of Akt and NF-κB p65, reduced the transfer of p65 to nuclear, and inhibited the activation of PI3K/Akt signaling pathway. The findings suggest that FEA exhibited an anti-inflammatory activity in vitro and could be used as a candidate in treatment of inflammation induced by PCV2 infection.

Keywords

3D4/2 cell
Anti-inflammatory activity
Polygonum hydropiper L
Porcine circovirus type 2
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Veterinary Research Forum
Volume 13, Issue 3
Summer 2022
Pages 339-347

  • Receive Date 06 October 2020
  • Revise Date 18 April 2021
  • Accept Date 08 May 2021

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