Veterinary Research Forum
Vet Res Forum

A novel competitive ELISA for detection of antibodies against bovine viral diarrhea virus infection

Document Type : Original Article

Authors

Masoud Reza Seyfi Abad Shapouri 1
Pezhman Mahmoodi 2
Masoud Ghorbanpour Najafabadi 3
Mohammad Rahim Haji Hajikolaei 4
Parastoo Moradi Choghakabodi 1
Mohsen Lotfi 5
Mahdi Pourmahdi Boroujeni 6
Maryam Ekhtelat 7
Maryam Daghari 1

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 Quality Control Department, Razi Vaccine and Serum Research Institute, Agricultural Education and Extension Research Organization (AREEO), Karaj, Iran

6 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

7 Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

https://doi.org/10.30466/vrf.2021.521500.3135
Abstract
Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS3), as a single ELISA recombinant antigen, along with a monoclonal antibody to detect antibodies against BVDV in sera of infected animals. Hence, 197 serum samples were tested by this cELISA and the results were compared to the results obtained from virus neutralization test (VNT) as the gold standard method for diagnosis of BVD. McNemar’s test indicated that there was no significant difference between the results of this newly designed cELISA and VNT. Meanwhile, kappa coefficients showed that there was a high correlation between these two assays. The relative sensitivity and specificity of cELISA with respect to VNT were 93.90% and 100%, respectively, suggesting that this newly designed cELISA could be a useful diagnostic tool for detection of BVDV infection. Moreover, as NS3 is highly conserved among Pestiviruses and the developed ELISA is a competitive one, it could potentially be applied to detect BVDV infection in other domestic and wildlife species.

Keywords

Bovine viral diarrhea
Competitive ELISA
Nonstructural protein 3
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Veterinary Research Forum
Volume 13, Issue 3
Summer 2022
Pages 403-407

  • Receive Date 30 January 2021
  • Accept Date 08 May 2021

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