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Pooyanfar, &., Foroutan, T., Dashtizad, M. (2018). Effects of blastocyst artificial collapse prior to vitrification on hatching and ‎survival rates and the expression of klf4 gene in mouse embryos. Veterinary Research Forum, 9(1), 87-92.
‎ Farkhondeh Pooyanfar; Tahereh Foroutan; Mojtaba Dashtizad. "Effects of blastocyst artificial collapse prior to vitrification on hatching and ‎survival rates and the expression of klf4 gene in mouse embryos". Veterinary Research Forum, 9, 1, 2018, 87-92.
Pooyanfar, &., Foroutan, T., Dashtizad, M. (2018). 'Effects of blastocyst artificial collapse prior to vitrification on hatching and ‎survival rates and the expression of klf4 gene in mouse embryos', Veterinary Research Forum, 9(1), pp. 87-92.
Pooyanfar, &., Foroutan, T., Dashtizad, M. Effects of blastocyst artificial collapse prior to vitrification on hatching and ‎survival rates and the expression of klf4 gene in mouse embryos. Veterinary Research Forum, 2018; 9(1): 87-92.

Effects of blastocyst artificial collapse prior to vitrification on hatching and ‎survival rates and the expression of klf4 gene in mouse embryos

Article 13, Volume 9, Issue 1, Winter 2018, Page 87-92  XML PDF (788.83 K)
Document Type: Short Communication
Authors
‎ Farkhondeh Pooyanfar1; Tahereh Foroutan email 1; Mojtaba Dashtizad2
1Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, ‎Tehran, Iran
2Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Abstract
Although the rate of blastocysts implantation of embryos is higher than previous stages but their survival rate is lower than them, which could be attributed to the completely filled blastocoel cavity with liquid and increased possibility of the formation of ice crystals. This liquid could prevent the penetration of cryoprotecting materials into the embryos. In this study, we reduced the volume of blastocoel before vitrification and compared survival rate and quality of in vitro embryos through klf4 gene expression with control group. In vitro mouse blastocysts were divided into three groups. In group 1, the blastocoel volume of blastocysts were reduced before vitrification and warming. In group 2, blastocysts were just vitrified and warmed and the blastocysts of group 3 (control group) were not undergone any specific treatment and were not vitrified. The expression ofklf4 gene was assessed using real-time PCR technique. Data were statistically analyzed using one-way ANOVA and Duncan’s post hoc tests. Our results showed that blastocoel volume reduction before vitrification significantly increased the hatching rate of the blastocysts from the zona pellucida and klf4 gene expression compared to vitrified group. Blastocoel volume reduction before vitrification could be used as an efficient method for improving the rate of in vitro fertilization.
Keywords
Artificial collapse; ‎ Blastocyst; ‎ In vitro fertilization; ‎ klf4 gene; ‎ Vitrification
Main Subjects
Embryology
References
 

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