Veterinary Research Forum

Vet Res Forum

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Protective effects of Nigella sativa on synaptic plasticity impairment induced ‎by lipopolysaccharide

Document Type : Original Article

Authors

1 Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran

2 Department of Physiology, Esfarayan Faculty of Medical Sciences, Esfarayan, Iran

3 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Basic Sciences and Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran‎

5 Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

6 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

In the present study the protective effect of Nigella sativa (N. sativa)on synaptic plasticity impairment induced by lipopolysaccharide (LPS) in rats was investigated. Fifty-eight rats were grouped and treated as follows: 1) control (saline), 2) LPS, 3) LPS-N. sativa,and 4) N. sativa. In a Morris water maze test, the escape latency and traveled path to find the platform as well as time spent and the traveled distance in target quadrant (Q1) were measured. Long term potentiation (LTP) from CA1 area of hippocampus followed by high frequency stimulation to Schafer collateral was studied and slope, slope 10-90% and amplitude of field excitatory field potential (fEPSP) were calculated. The escape latency and traveled path in LPS group were significantly higher than those in the control group while, in LPS-N. sativa group these parameters were significantly lower than those in LPS group. The rats in LPS group spent less time and traveled shorter distance in Q1 than the rats in the control group while, in LPS-N. sativa group the rats spent more time and traveled longer distance than the rats in LPS group. LPS significantly decreased slope, slope 10-90% and amplitude of fEPSP while, in LPS-N. sativa group these parameters increased compared to LPS group. The results indicated that the hydro-alcohol extract of N. sativa protected against synaptic plasticity and spatial learning and memory impairment induced by LPS in rats.

Keywords

Main Subjects

References

  1.  

    1. Yirmiya R, Goshen I. Immune modulation of learning, memory, neural plasticity and neurogenesis. Brain Behav Immun 2011; 25(2): 181-213.
    2. Goshen I, Avital A, Kreisel T, et al. Environmental enrichment restores memory functioning in mice with impaired IL-1 signaling via reinstatement of long-term potentiation and spine size enlargement. J Neurosci 2009; 29(11): 3395-3403.
    3. Tha KK, Okuma Y, Miyazaki H, et al. Changes in expressions of proinflammatory cytokines IL-1β, TNF-α and IL-6 in the brain of senescence accelerated mouse (SAM) P8. Brain Res 2000; 885(1): 25-31.
    4. Sell KM, Crowe SF, Kent S. Lipopolysaccharide induces memory-processing deficits in day-old chicks. Pharmacol Biochem Behav 2001; 68(3): 497-502.
    5. Lynch MA. Interleukin-1β exerts a myriad of effects in the brain and in particular in the hippocampus: analysis of some of these actions. Vitam Horm 2002; 64: 185-219.
    6. Curran BP, O'Connor JJ. The inhibition of long-term potentiation in the rat dentate gyrus by pro-inflammatory cytokines is attenuated in the presence of nicotine. Neurosci lett 2003; 344(2): 103-106.
    7. Lee JW, Lee YK, Yuk DY, et al. Neuro-inflammation induced by lipopolysaccharide causes cognitive impairment through enhancement of beta-amyloid generation. J Neuroinflammation 2008; doi:10.1186/ 1742-2094-5-37.
    8. Luk WP, Zhang Y, White TD, et al. Adenosine: A mediator of interleukin-1β-induced hippocampal synaptic inhibition. J Neurosci 1999; 19(11): 4238-4244.
    9. Commins S, O'Neill L, O'Mara S. The effects of the bacterial endotoxin lipopolysaccharide on synaptic transmission and plasticity in the CA1-subiculum pathway in vivo. Neuroscience 2001; 102(2): 273-280.
    10. Ahmad A, Husain A, Mujeeb M, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed 2013; 3(5): 337-352.
    11. Vafaee F, Hosseini M, Hassanzadeh Z, et al. The effects of Nigella sativa hydro-alcoholic extract on memory and brain tissues oxidative damage after repeated seizures in rats. Iran J Pharm Res 2015; 14(2): 547-557.‎
    12. Umar S, Zargan J, Umar K, et al. Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats. Chem Biol Interact 2012; 197(1): 40-46.
    13. Azzubaidi MS, Saxena AK, A Talib N, et al. Protective effect of treatment with black cumin oil on spatial cognitive functions of rats that suffered global cerebrovascular hypoperfusion. Acta Neurobiol Exp 2012; 72(2): 154-165.
    14. Sayeed MSB, Asaduzzaman M, Morshed H, et al. The effect of Nigella sativa Linn. seed on memory, attention and cognition in healthy human volunteers. J Ethnopharmacol 2013; 148(3): 780-786.
    15. Jalali MR, Roghani M. The effect of Nigella sativa on learning and memory in male diabetic rats. Basic Clin Neurosci 2009; 1(1): 32-34.
    16. Salama SM, Abdulla MA, Al-Rashdi AS, et al. Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats. BMC Complement Altern Med 2013; doi: 10.1186/1472-6882-13-56.
    17. Mohammadpour T, Hosseini M, Naderi A, et al. Protection against brain tissues oxidative damage as a possible mechanism for the beneficial effects of Rosa damascena hydroalcoholic extract on scopolamine induced memory impairment in rats. Nutr Neurosc 2015; 18(7): 329-336.
    18. Anaeigoudari A, Soukhtanloo M, Reisi P, et al. Inducible nitric oxide inhibitor aminoguanidine, ameliorates deleterious effects of lipopolysaccharide on memory and long term potentiation in rat. Life Sci 2016; 158: 22-30.
    19. Saadati H, Sheibani V, Esmaeili-Mahani S, et al. Prior regular exercise prevents synaptic plasticity impairment in sleep deprived female rats. Brain Res Bull 2014; 108: 100-105.
    20. Anaeigoudari A, Shafei MN, Soukhtanloo M, et al. The effects of L-arginine on spatial memory and synaptic plasticity impairments induced by lipopolysaccharide. Adv Biomed Res 2015; 4:202.
    21. Anaeigoudari A, Soukhtanloo M, Shafei MN, et al. Neuronal nitric oxide synthase has a role in the detrimental effects of lipopolysaccharide on spatial memory and synaptic plasticity in rats. Pharmacol Rep 2016; 68(2): 243-249.
    22. Shaw KN, Commins S, O'Mara SM. Lipopolysaccharide causes deficits in spatial learning in the watermaze but not in BDNF expression in the rat dentate gyrus. Behav Brain Res 2001; 124(1): 47-54.
    23. Hauss-Wegrzyniak B, Lynch M, Vraniak P, et al. Chronic brain inflammation results in cell loss in the entorhinal cortex and impaired LTP in perforant path-granule cell synapses. Exp Neurol 2002; 176(2): 336-341.
    24. Maier SF, Watkins LR. Cytokines for psychologists: Implications of bidirectional immune-to-brain communication for understanding behavior, mood, and cognition. Psychol Rev 1998; 105(1): 83-107.
    25. Coogan A, O'Connor JJ. Inhibition of NMDA receptor‐mediated synaptic transmission in the rat dentate gyrus in vitro by IL‐1β. Neuroreport 1997; 8(9): 2107-2110.
    26. Fedulov V, Rex CS, Simmons DA, et al. Evidence that long-term potentiation occurs within individual hippocampal synapses during learning. J Neurosci 2007; 27(30): 8031-8039.
    27. Rosczyk H, Sparkman N, Johnson R. Neuro-inflammation and cognitive function in aged mice following minor surgery. Exp Gerontol 2008; 43(9): 840-846.
    28. Collingridge G, Bliss T. NMDA receptors-their role in long-term potentiation. Trends Neurosci 1987; 10(7): 288-293.
    29. Wu J, Rush A, Rowan MJ, et al. NMDA receptor‐and metabotropic glutamate receptor‐dependent synaptic plasticity induced by high frequency stimulation in the rat dentate gyrus in vitro. J Physiol 2001; 533(3): 745-755.
    30. Bliss TV, Collingridge GL. A synaptic model of memory: long-term potentiation in the hippocampus. Nature 1993; 361(6407): 31-39.
    31. Cunningham A, Murray C, O'Neill L, et al. Interleukin-1β (IL-1β) and tumour necrosis factor (TNF) inhibit long-term potentiation in the rat dentate gyrus in vitro. Neurosci Lett 1996; 203(1): 17-20.
    32. Manabe T, Renner P, Nicoll RA. Postsynaptic contribution to long-term potentiation revealed by the analysis of miniature synaptic currents. Nature 1992; 355(6355): 50-55.
    33. Maren S, Baudry M, Thompson RF. Effects of the novel NMDA receptor antagonist, CGP 39551, on field potentials and the induction and expression of LTP in the dentate gyrus in vivo. Synapse 1992; 11(3): 221-228.
    34. El-Naggar T, Gómez-Serranillos MP, Palomino OM, et al. Nigella sativa L. seed extract modulates the neurotransmitter amino acids release in cultured neurons in vitro. J Biomed Biotechnol 2010; 2010. dx.doi.org/10.1155/2010/398312.
    35. Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immuno-pharmacol 2005; 5(13): 1749-1770.
    36. Islam MT, Sultana N, Riaz TA, et al. Thymoquinone is knocking at the door of clinical trial. Int Arch Med 2016; 9(122). dx.doi.org/10.3823/1993.
    37. Majdalawieh AF, Hmaidan R, Carr RI. Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J Ethnopharmacol 2010; 131(2): 268-275.
    38. Umar S, Hedaya O, Singh AK et al. Thymoquinone inhibits TNF-α-induced inflammation and cell adhesion in rheumatoid arthritis synovial fibroblasts by ASK1 regulation. Taxicol Appl Pharmacol 2015; 287(3):
      299-305.
    39. Kanter M, Coskun O, Kalayc M, et al. Neuroprotective effects of Nigella sativa on experimental spinal cord injury in rats. Hum Exp Toxicol 2006; 25(3): 127-133.
    40. Sandhu KS, Rana AC. Evaluation of anti-parkinson’s activity of Nigella sativa (kalonji) seeds in chlor-promazine induced experimental animal model. Int J Pharm Pharm Sci 2013; 5:884-888.
    41. Hosseinzadeh H, Parvardeh S, Asl MN, et al. Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia-reperfusion injury in rat hippocampus. Phytomedicine 2007; 14(9): 621-627.
    42. Beheshti F, Hosseini M, Shafei MN, et al. The effects of Nigella sativa extract on hypothyroidism-associated learning and memory impairment during neonatal and juvenile growth in rats. Nutr Neurosci 2017; 20(1): 49-59.
    43. Beheshti F, Hosseini M, Vafaee F, et al. Feeding of Nigella sativa during neonatal and juvenile growth improves learning and memory of rats. J Tradit Complement Med 2016; 6(2): 146-152.
Veterinary Research Forum
Volume 9, Issue 1
March 2018
Pages 27-33
Files
History
  • Receive Date: 05 April 2017
  • Revise Date: 05 November 2017
  • Accept Date: 07 November 2017
Share
How to cite
Statistics