Veterinary Research Forum
Vet Res Forum

Protective effects of Nigella sativa on synaptic plasticity impairment induced ‎by lipopolysaccharide

Document Type : Original Article

Authors

Akbar Anaeigoudari 1
Fatemeh Norouzi 2
Azam Abareshi 3
Farimah Beheshti 4, 5
Azita Aaghaei 3
Mohammad Naser Shafei 6
Zahra Gholamnezhad 6
Mahmoud Hosseini 5

1 Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran

2 Department of Physiology, Esfarayan Faculty of Medical Sciences, Esfarayan, Iran

3 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Basic Sciences and Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran‎

5 Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

6 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract
In the present study the protective effect of Nigella sativa (N. sativa)on synaptic plasticity impairment induced by lipopolysaccharide (LPS) in rats was investigated. Fifty-eight rats were grouped and treated as follows: 1) control (saline), 2) LPS, 3) LPS-N. sativa,and 4) N. sativa. In a Morris water maze test, the escape latency and traveled path to find the platform as well as time spent and the traveled distance in target quadrant (Q1) were measured. Long term potentiation (LTP) from CA1 area of hippocampus followed by high frequency stimulation to Schafer collateral was studied and slope, slope 10-90% and amplitude of field excitatory field potential (fEPSP) were calculated. The escape latency and traveled path in LPS group were significantly higher than those in the control group while, in LPS-N. sativa group these parameters were significantly lower than those in LPS group. The rats in LPS group spent less time and traveled shorter distance in Q1 than the rats in the control group while, in LPS-N. sativa group the rats spent more time and traveled longer distance than the rats in LPS group. LPS significantly decreased slope, slope 10-90% and amplitude of fEPSP while, in LPS-N. sativa group these parameters increased compared to LPS group. The results indicated that the hydro-alcohol extract of N. sativa protected against synaptic plasticity and spatial learning and memory impairment induced by LPS in rats.

Keywords

Lipopolysaccharide
‎ Long term potentiation
Nigella sativa L
Spatial memory
Synaptic plasticity

Subjects

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Veterinary Research Forum
Volume 9, Issue 1
Winter 2018
Pages 27-33

  • Receive Date 05 April 2017
  • Revise Date 05 November 2017
  • Accept Date 07 November 2017

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